LOCUS       V015906                 6075 bp    DNA     circular SYN 25-MAR-2019
DEFINITION  Exported.
ACCESSION   V015906
VERSION     V015906
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2018) State Key Laboratory of Food Science and
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su
            214122, China
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (05-DEC-2018) State Key Laboratory of Food Science and Technology,
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
FEATURES             Location/Qualifiers
     source          1..6075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label="rrnG antiterminator"
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     CDS             383..406
                     /label="minicistron"
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
     CDS             425..442
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             446..478
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             482..505
                     /label="Xpress(TM) tag"
                     /note="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
     CDS             521..1264
                     /gene="xylB"
                     /label="D-xylose 1-dehydrogenase"
                     /note="D-xylose 1-dehydrogenase from Caulobacter vibrioides
                     (strain ATCC 19089 / CB15). Accession#: Q9A9Z0"
     misc_feature    1304..1321
                     /label="TEVp cleave site"
                     /note="TEVp cleave site"
     misc_feature    1322..1342
                     /label="F degron"
                     /note="F degron"
     CDS             1343..2209
                     /gene="xylC"
                     /label="D-xylonolactone lactonase"
                     /note="D-xylonolactone lactonase from Caulobacter
                     vibrioides (strain ATCC 19089 / CB15). Accession#: Q9A9Z1"
     terminator      2428..2514
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2606..2633
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2653..2744
                     /label="AmpR promoter"
     CDS             2745..3602
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3776..4364
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(4550..4690)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     promoter        4876..4953
                     /label="lacI promoter"
     CDS             4954..6033
                     /label="lacI"
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
      301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
      361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
      421 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
      481 ggatctgtac gacgatgacg ataaggatcg atggggatcc atgtcttctg ctatctaccc
      541 gtctctgaaa ggtaaacgtg ttgttatcac cggtggtggt tctggtatcg gtgctggtct
      601 gaccgctggt ttcgctcgtc agggtgctga agttatcttc ctggacatcg ctgacgaaga
      661 ctctcgtgct ctggaagctg aactggctgg ttctccgatc ccgccggttt acaaacgttg
      721 cgacctgatg aacctggaag ctatcaaagc tgttttcgct gaaatcggtg acgttgacgt
      781 tctggttaac aacgctggta acgacgaccg tcacaaactg gctgacgtta ccggtgctta
      841 ctgggacgaa cgtatcaacg ttaacctgcg tcacatgctg ttctgcaccc aggctgttgc
      901 tccgggtatg aaaaaacgtg gtggtggtgc tgttatcaac ttcggttcta tctcttggca
      961 cctgggtctg gaagacctgg ttctgtacga aaccgctaaa gctggtatcg aaggtatgac
     1021 ccgtgctctg gctcgtgaac tgggtccgga cgacatccgt gttacctgcg ttgttccggg
     1081 taacgttaaa accaaacgtc aggaaaaatg gtacaccccg gaaggtgaag ctcagatcgt
     1141 tgctgctcag tgcctgaaag gtcgtatcgt tccggaaaac gttgctgctc tggttctgtt
     1201 cctggcttct gacgacgctt ctctgtgcac cggtcacgaa tactggatcg acgctggttg
     1261 gcgttaagag ctcaaagagg agaaagtcga catggcagat ctggaaaatc tgtattttca
     1321 gtttctgttt gtgcaagaac tgatgaccgc ccaagttact tgtgtttggg atctgaaagc
     1381 caccttaggc gaaggtccta tctggcatgg cgatacttta tggttcgtgg acatcaaaca
     1441 gcgcaaaatc cacaattatc acccggccac tggtgagcgt ttcagtttcg atgcaccgga
     1501 ccaagttacc tttttagccc cgattgtggg cgccaccggc tttgtggtgg gtctgaaaac
     1561 cggcatccac cgttttcatc cggcaaccgg cttcagctta ctgctggaag tggaagatgc
     1621 cgcactgaac aaccgcccga acgacgccac agtggatgca caaggtcgtt tatggtttgg
     1681 caccatgcac gatggtgagg aaaacaatag cggctcttta tatcgcatgg atctgaccgg
     1741 tgtggcacgc atggaccgtg acatttgcat taccaacggc ccgtgtgtga gcccggacgg
     1801 caaaaccttt taccacaccg acactttaga gaagaccatc tacgccttcg atctggcaga
     1861 agacggttta ctgagtaaca agcgcgtgtt cgttcagttt gctttaggtg atgatgtgta
     1921 tccggacggc agcgtggtgg atagtgaagg ctatctgtgg accgcactgt ggggcggttt
     1981 cggtgcagtg cgctttagcc cgcaaggtga tgccgttacc cgcattgaac tgccggcacc
     2041 gaacgtgaca aaaccgtgct ttggtggtcc ggatttaaaa actttatact tcaccaccgc
     2101 ccgtaaaggt ttaagtgacg aaactttagc acaatacccg ctggctggtg gcgtttttgc
     2161 agttcccgtt gatgtggccg gccaaccgca gcatgaagtg cgtctggttt aagaattcga
     2221 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
     2281 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     2341 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     2401 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2461 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     2521 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     2581 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     2641 ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2701 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2761 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2821 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2881 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2941 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     3001 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3061 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3121 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3181 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3241 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3301 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3361 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3421 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3481 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3541 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3601 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3661 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3721 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3781 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3841 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3901 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3961 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4021 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4081 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4141 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4201 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4261 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4321 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4381 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4441 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4501 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     4561 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     4621 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     4681 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4741 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4801 ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg
     4861 gcatgcattt acgttgacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag
     4921 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc
     4981 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
     5041 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
     5101 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
     5161 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
     5221 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
     5281 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
     5341 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
     5401 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
     5461 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
     5521 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
     5581 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
     5641 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
     5701 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
     5761 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtc aaccaccatc
     5821 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     5881 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     5941 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6001 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6061 gcgcgaattg atctg
//
