LOCUS       pTrcHis2_A.        4406 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of C-terminally 
            6xHis-tagged proteins. For other reading frames, use pTrcHis2 B or 
            pTrcHis2 C.
ACCESSION   .
VERSION     .
KEYWORDS    pTrcHis2 A.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     misc_feature    346..354
                     /label=gene 10 translational enhancer
                     /note="gene 10 translational enhancer"
                     /note="translational enhancer from gene 10 of bacteriophage
                     T7 (Olins and Rangwala, 1989)"
     RBS             369..373
                     /note="ribosome binding site"
     CDS             383..406
                     /label=minicistron
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial 
                     expression of a downstream cistron (Schoner, 1997)"
     misc_feature    411..464
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             413..415
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             471..500
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             516..533
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      759..845
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      937..964
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        984..1075
                     /label=AmpR promoter
     CDS             1076..1933
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2107..2695
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2881..3021)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3207..3284
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3285..4364
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
      301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
      361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatggatcc
      421 gagctcgaga tctgcagctg gtaccatatg ggaattcgaa gcttgggccc gaacaaaaac
      481 tcatctcaga agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt
      541 aaacggtctc cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
      601 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
      661 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
      721 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      781 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      841 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
      901 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
      961 tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa atatgtatcc
     1021 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1081 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1141 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1201 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1261 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     1321 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1381 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1441 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1501 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1561 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1621 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1681 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1741 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1801 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1861 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1921 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1981 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2041 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2101 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2161 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2221 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2281 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2341 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2401 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2461 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2521 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2581 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2641 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2701 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2761 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2821 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2881 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     2941 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     3001 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3061 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3121 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg
     3181 aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta
     3241 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     3301 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     3361 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     3421 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     3481 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     3541 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     3601 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     3661 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     3721 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     3781 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     3841 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     3901 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     3961 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     4021 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     4081 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     4141 caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     4201 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     4261 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4321 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4381 aatgtgagtt agcgcgaatt gatctg
//
