LOCUS       pTrcHis_C.        4412 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of N-terminally 
            6xHis-tagged proteins. For other reading frames, use pTrcHis A or 
            pTrcHis B.
ACCESSION   .
VERSION     .
KEYWORDS    pTrcHis C.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     misc_feature    346..354
                     /label=gene 10 translational enhancer
                     /note="gene 10 translational enhancer"
                     /note="translational enhancer from gene 10 of bacteriophage
                     T7 (Olins and Rangwala, 1989)"
     RBS             369..373
                     /note="ribosome binding site"
     CDS             383..406
                     /label=minicistron
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial 
                     expression of a downstream cistron (Schoner, 1997)"
     CDS             413..415
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             425..442
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             446..478
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             482..505
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    513..562
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      765..851
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      943..970
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        990..1081
                     /label=AmpR promoter
     CDS             1082..1939
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2113..2701
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2887..3027)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3213..3290
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3291..4370
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
      301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
      361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
      421 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
      481 ggatctgtac gacgatgacg ataagcatcg atggatccga cctcgagatc tgcagctggt
      541 accatatggg aattcgaagc ttggctgttt tggcggatga gagaagattt tcagcctgat
      601 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
      661 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
      721 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
      781 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      841 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
      901 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
      961 atggcctttt tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat
     1021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1081 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1201 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1261 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1321 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1381 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1501 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1621 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1681 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1921 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1981 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2221 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2881 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     2941 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     3001 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     3061 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3121 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg
     3181 cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc
     3241 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     3301 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     3361 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     3421 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     3481 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     3541 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     3601 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     3661 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     3721 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     3781 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     3841 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     3901 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     3961 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     4021 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     4081 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     4141 cgccgtcaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     4201 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     4261 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4321 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4381 gcaattaatg tgagttagcg cgaattgatc tg
//
