LOCUS       40924_43943        3708 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrcCDFS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 3708)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..204
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    212..228
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    252..308
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      306..453
                     /label=rrnBT1T2
                     /note="rrnBT1T2"
                     /regulatory_class="terminator"
     CDS             complement(627..1415)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(1416..1507)
                     /label=AmpR promoter
     rep_origin      complement(1555..2293)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(2469..2490)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2506..3585)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3586..3663)
                     /label=lacI promoter
ORIGIN
        1 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
       61 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
      121 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
      181 attaatcatc cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga
      241 aacagaccat ggaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat
      301 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
      361 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
      421 ctgaccccat gccgaactca gaagtgaaac gccctgaaac ctcaggcatt tgagaagcac
      481 acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct
      541 atttaacgac cctgccctga accgacgacc gggtcatcgt ggccggatct tgcggcccct
      601 cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg
      661 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
      721 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
      781 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
      841 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
      901 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
      961 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
     1021 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
     1081 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
     1141 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
     1201 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
     1261 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
     1321 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
     1381 tgagtcgata cttcggcgat caccgcttcc ctcatactct tcctttttca atattattga
     1441 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1501 aaacaaatag ctagctcact cggtcgctac gctccgggcg tgagactgcg gcgggcgctg
     1561 cggacacata caaagttacc cacagattcc gtggataagc aggggactaa catgtgaggc
     1621 aaaacagcag ggccgcgccg gtggcgtttt tccataggct ccgccctcct gccagagttc
     1681 acataaacag acgcttttcc ggtgcatctg tgggagccgt gaggctcaac catgaatctg
     1741 acagtacggg cgaaacccga caggacttaa agatccccac cgtttccggc gggtcgctcc
     1801 ctcttgcgct ctcctgttcc gaccctgccg tttaccggat acctgttccg cctttctccc
     1861 ttacgggaag tgtggcgctt tctcatagct cacacactgg tatctcggct cggtgtaggt
     1921 cgttcgctcc aagctgggct gtaagcaaga actccccgtt cagcccgact gctgcgcctt
     1981 atccggtaac tgttcacttg agtccaaccc ggaaaagcac ggtaaaacgc cactggcagc
     2041 agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg
     2101 aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa
     2161 agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc
     2221 ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga
     2281 agatcctttg atcttttcta ctgaaccgct ctagatttca gtgcaattta tctcttcaaa
     2341 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt agtcatgccc
     2401 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     2461 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     2521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2581 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     2641 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     2701 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     2761 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     2821 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     2881 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     2941 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     3001 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     3061 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     3121 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     3181 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     3241 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     3301 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     3361 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     3421 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     3481 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     3541 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     3601 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     3661 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaat
//
