LOCUS       40924_43928        5629 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-zwf, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5629)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5629)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1739
                     /gene="zwf"
                     /label=zwf
                     /note="Glucose-6-phosphate 1-dehydrogenase from Escherichia
                     coli (strain K12). Accession#: P0AC53"
     terminator      1982..2068
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2160..2187
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2207..2298
                     /label=AmpR promoter
     CDS             2299..3156
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3330..3918
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4104..4244)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4430..4507
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4508..5587
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgg cggtaacgca aacagcccag gcctgtgacc
      301 tggtcatttt cggcgcgaaa ggcgaccttg cgcgtcgtaa attgctgcct tccctgtatc
      361 aactggaaaa agccggtcag ctcaacccgg acacccggat tatcggcgta gggcgtgctg
      421 actgggataa agcggcgtat accaaagttg tccgcgaggc gctcgaaact ttcatgaaag
      481 aaaccattga tgaaggttta tgggacaccc tgagtgcacg tctggatttt tgtaatctcg
      541 atgtcaatga cactgctgca ttcaaccgtc tcggcgcgat gctggatcaa aaaaatcgta
      601 tcaccattaa ctactttgcc atgccgccca gcacttttgg cgcaatttgc aaagggcttg
      661 gcgaggcaaa actgaatgct aaaccggcac gcgtagtcat ggagaaaccg ctggggacgt
      721 cgctggcgac ctcgcaggaa atcaatgatc aggttggcga atacttcgag gagtgccagg
      781 tttaccgtat cgaccactat cttggtaaag aaacggtgct gaacctgttg gcgctgcgtt
      841 ttgctaactc cctgtttgtg aataactggg acaatcgcac cattgatcat gttgagatta
      901 ccgtggcaga agaagtgggg atcgaagggc gctggggcta ttttgataaa gccggtcaga
      961 tgcgcgatat gatccaaaac cacctgctgc aaattctctg catgattgcg atgtctccgc
     1021 catctgacct gagcgcagac agcatccgcg atgaaaaagt gaaagtactg aagtccctgc
     1081 gccgcatcga ccgctccaac gtacgcgaaa aaaccgtacg tgggcaatat actgcgggct
     1141 tcgcccaggg caaaaaagtg ccgggatatc tggaagaaga gggcgcgaac aagagcagca
     1201 atacagaaac cttcgtggcg atccgcgtcg acattgataa ctggcgctgg gccggtgtgc
     1261 cattctacct gcgtactggt aaacgtctgc cgaccaaatg ttctgaagtc gtggtctatt
     1321 tcaaaacacc tgaactgaat ctgtttaaag agtcgtggca ggatctgccg cagaataaac
     1381 tgactatccg tctgcaacct gatgaaggcg tggatatcca ggtactgaat aaagttcctg
     1441 gccttgacca caaacataac ctgcaaatca ccaagctgga tctgagctat tcagaaacct
     1501 ttaatcagac gcatctggcg gatgcctatg aacgtctgct gctggaaacc atgcgtggta
     1561 ttcaggcact gtttgttcgt cgcgacgaag tggaagaagc ctggaaatgg gtagactcca
     1621 ttactgaggc gtgggcgatg gacaatgatg cgccgaaacc gtatcaggcc ggaacctggg
     1681 gacccgttgc ctcggtggcg atgattaccc gtgatggtcg ttcctggaat gagtttgagt
     1741 aatggatcct ctagagtcga cctgcaggca tgcaagcttg gctgttttgg cggatgagag
     1801 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     1861 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
     1921 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
     1981 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2041 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     2101 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca
     2161 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actctttttg tttatttttc
     2221 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2281 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2341 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2401 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2461 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2521 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     2581 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     2641 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     2701 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2761 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2821 gagcgtgaca ccacgatgcc tacagcaatg gcaacaacgt tgcgcaaact attaactggc
     2881 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2941 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3001 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3061 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3121 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3181 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3241 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3301 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3481 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3541 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3601 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3661 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3721 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3781 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3841 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3901 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3961 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4021 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4081 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4141 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4201 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     4261 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4321 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4381 ggcagcagat caattcgcgc gcgaaggcga agcggcatgc atttacgttg acaccatcga
     4441 atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
     4501 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
     4561 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     4621 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
     4681 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     4741 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     4801 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     4861 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     4921 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     4981 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     5041 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     5101 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     5161 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     5221 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     5281 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
     5341 cagctcatgt tatatcccgc cgtcaaccac catcaaacag gattttcgcc tgctggggca
     5401 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     5461 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     5521 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     5581 cgggcagtga gcgcaacgca attaatgtga gttagcgcga attgatctg
//
