LOCUS       40924_43918        4921 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-tpiA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 4921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1031
                     /gene="tpiA"
                     /label=tpiA
                     /note="Triosephosphate isomerase from Escherichia coli
                     (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / 
                     WDCM 00012 / Crooks). Accession#: B1IVG0"
     terminator      1274..1360
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1452..1479
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1499..1590
                     /label=AmpR promoter
     CDS             1591..2448
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2622..3210
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3396..3536)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3722..3799
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3800..4879
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgc gacatccttt agtgatgggt aactggaaac
      301 tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa gagctggcag
      361 gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat atggcgaagc
      421 gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg
      481 gcgcattcac cggtgaaacc tctgctgcta tgctgaaaga catcggcgca cagtacatca
      541 tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg atcgcgaaaa
      601 aattcgcggt gctgaaagag cagggcctga ctccggttct gtgcatcggt gaaaccgaag
      661 ctgaaaacga agcgggcaaa actgaagaag tttgcgcacg tcagatcgac gcggtactga
      721 aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaaccc gtatgggcaa
      781 tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa ttcattcgtg
      841 accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag tacggcggct
      901 ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agacatcgac ggcgcgctgg
      961 ttggcggtgc ttctctgaaa gctgacgctt tcgcagtaat cgttaaagct gcagaagcgg
     1021 ctaaacaggc ttaatggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt
     1081 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     1141 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     1201 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
     1261 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1321 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     1381 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     1441 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt
     1501 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1561 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1621 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1681 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1741 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1801 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     1861 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1921 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1981 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2041 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2101 ataccaaacg acgagcgtga caccacgatg cctacagcaa tggcaacaac gttgcgcaaa
     2161 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2221 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2281 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2341 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2401 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2461 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2521 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2761 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3241 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3301 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3361 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     3421 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     3481 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     3541 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3601 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3661 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt
     3721 tgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
     3781 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     3841 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     3901 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     3961 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     4021 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     4081 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     4141 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     4201 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     4261 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     4321 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     4381 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     4441 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     4501 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     4561 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     4621 cgataccgaa gacagctcat gttatatccc gccgtcaacc accatcaaac aggattttcg
     4681 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     4741 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     4801 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4861 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagcgc gaattgatct
     4921 g
//
