LOCUS       40924_43908        5242 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-serC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5242)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5242)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1352
                     /gene="serC"
                     /label=serC
                     /note="Phosphoserine aminotransferase from Escherichia coli
                     (strain 55989 / EAEC). Accession#: B7LD99"
     terminator      1595..1681
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1773..1800
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1820..1911
                     /label=AmpR promoter
     CDS             1912..2769
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2943..3531
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3717..3857)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4043..4120
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4121..5200
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgg ctcaaatctt caattttagt tctggtccgg
      301 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
      361 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
      421 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
      481 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
      541 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
      601 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
      661 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
      721 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
      781 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
      841 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
      901 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
      961 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     1021 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     1081 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     1141 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     1201 tgttccttga agagtctttt gctgctggcc ttcatgcact gaaaggtcac cgtgtggtcg
     1261 gcggaatgcg cgcgtctatt tataacgcca tgccgctgga aggcgttaaa gcgctgacag
     1321 acttcatggt tgagttcgaa cgccgtcacg gctaatggat cctctagagt cgacctgcag
     1381 gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     1441 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     1501 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     1561 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     1621 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1681 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     1741 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     1801 tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1861 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1921 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1981 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2041 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2101 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     2161 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2221 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2281 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2341 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2401 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctacagca
     2461 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2521 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2581 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2641 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2701 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2761 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2821 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2881 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2941 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3001 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3061 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3721 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     3781 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     3841 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3961 agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg
     4021 cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc
     4081 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     4141 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     4201 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     4261 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     4321 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     4381 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     4441 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     4501 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     4561 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     4621 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     4681 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     4741 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     4801 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     4861 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     4921 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac
     4981 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     5041 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     5101 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5161 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5221 tgagttagcg cgaattgatc tg
//
