LOCUS       40924_43903        5122 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-serB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5122)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5122)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1232
                     /gene="serB"
                     /label=serB
                     /note="Phosphoserine phosphatase from Escherichia coli
                     (strain K12). Accession#: P0AGB0"
     terminator      1475..1561
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1653..1680
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1700..1791
                     /label=AmpR promoter
     CDS             1792..2649
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2823..3411
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3597..3737)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3923..4000
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4001..5080
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgc ctaacattac ctggtgcgac ctgcctgaag
      301 atgtctcttt atggccgggt ctgcctcttt cattaagtgg tgatgaagtg atgccactgg
      361 attaccacgc aggtcgtagc ggctggctgc tgtatggtcg tgggctggat aaacagcgtc
      421 tgacccaata ccagagcaaa ctgggtgcgg cgatggtgat tgttgccgcc tggtgcgtgg
      481 aagattatca ggtgattcgt ctggcaggtt cactcaccgc acgggctacg cgcctggccc
      541 atgaagcgca actggatgtc gccccgctgg ggaaaatccc gcacctgcgc acgccgggtt
      601 tgctggtgat ggacatggat tccaccgcca tccagattga atgtattgat gaaattgcca
      661 aactggccgg aacgggcgag atggtggcgg aagtaaccga acgggcgatg cgcggcgaac
      721 tcgattttac cgccagcctg cgcagccgcg tggcgacgct gaaaggcgct gacgccaata
      781 ttctgcaaca ggtgcgtgaa aatctgccgc tgatgccagg cttaacgcaa ctggtgctca
      841 agctggaaac gctgggctgg aaagtggcga ttgcctccgg cggctttact ttctttgctg
      901 aatatctgcg cgacaagttg cgcctgaccg ccgtggtagc caatgaactg gagatcatgg
      961 acggtaaatt taccggcaat gtgatcggcg acatcgtaga cgcgcagtac aaagcgaaaa
     1021 ctctgactcg cctcgcgcag gagtatgaaa tcccgctggc gcagaccgtg gcgattggcg
     1081 atggagccaa tgacctgccg atgatcaaag cggcagggct ggggattgcc taccatgcca
     1141 agccaaaagt gaatgaaaag gcggaagtca ccatccgtca cgctgacctg atgggggtat
     1201 tctgcatcct ctcaggcagc ctgaatcaga agtaatggat cctctagagt cgacctgcag
     1261 gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     1321 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     1381 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     1441 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     1501 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1561 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     1621 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     1681 tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1741 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1801 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1861 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1921 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1981 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     2041 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2101 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2161 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2221 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2281 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctacagca
     2341 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2401 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2461 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2521 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2581 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2641 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2701 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2761 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2941 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3601 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     3661 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     3721 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3781 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3841 agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg
     3901 cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc
     3961 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     4021 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     4081 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     4141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     4201 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     4261 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     4321 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     4381 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     4441 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     4501 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     4561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     4621 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     4681 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     4741 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     4801 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac
     4861 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     4921 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     4981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5041 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5101 tgagttagcg cgaattgatc tg
//
