LOCUS       40924_43898        5386 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-serA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5386)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5386)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1496
                     /gene="serA"
                     /label=serA
                     /note="D-3-phosphoglycerate dehydrogenase from Escherichia
                     coli (strain K12). Accession#: P0A9T0"
     terminator      1739..1825
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1917..1944
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1964..2055
                     /label=AmpR promoter
     CDS             2056..2913
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3087..3675
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3861..4001)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4187..4264
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4265..5344
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgg caaaggtatc gctggagaaa gacaagatta
      301 agtttctgct ggtagaaggc gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt
      361 acaccaacat cgaatttcac aaaggcgcgc tggatgatga acaattaaaa gaatccatcc
      421 gcgatgccca cttcatcggc ctgcgatccc gtacccatct gactgaagac gtgatcaacg
      481 ccgcagaaaa actggtcgct attggctgtt tctgtatcgg aacaaaccag gttgatctgg
      541 atgcggcggc aaagcgcggg atcccggtat ttaacgcacc gttctcaaat acgcgctctg
      601 ttgcggagct ggtgattggc gaactgctgc tgctattgcg cggcgtgccg gaagccaatg
      661 ctaaagcgca ccgtggcgtg tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca
      721 aaaagctggg tatcatcggc tacggtcata ttggtacgca attgggcatt ctggctgaat
      781 cgctgggaat gtatgtttac ttttatgata ttgaaaataa actgccgctg ggcaacgcca
      841 ctcaggtaca gcatctttct gacctgctga atatgagcga tgtggtgagt ctgcatgtac
      901 cagagaatcc gtccaccaaa aatatgatgg gcgcgaaaga aatttcacta atgaagcccg
      961 gctcgctgct gattaatgct tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg
     1021 cgctggcgag caaacatctg gcgggggcgg caatcgacgt attcccgacg gaaccggcga
     1081 ccaatagcga tccatttacc tctccgctgt gtgaattcga caacgtcctt ctgacgccac
     1141 acattggcgg ttcgactcag gaagcgcagg agaatatcgg cctggaagtt gcgggtaaat
     1201 tgatcaagta ttctgacaat ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc
     1261 tgccactgca cggtgggcgt cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa
     1321 ctgcgctgaa caaaatcttc gccgagcagg gcgtcaacat cgccgcgcaa tatctgcaaa
     1381 cttccgccca gatgggttat gtggttattg atattgaagc cgacgaagac gttgccgaaa
     1441 aagcgctgca ggcaatgaaa gctattccgg gtaccattcg cgcccgtctg ctgtactaat
     1501 ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag
     1561 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
     1621 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     1681 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
     1741 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1801 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
     1861 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
     1921 ggccatcctg acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa
     1981 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2041 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2101 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2161 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2221 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2281 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     2341 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2401 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2461 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2521 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2581 cgtgacacca cgatgcctac agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2641 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2701 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     2761 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2821 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2881 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2941 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3001 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3061 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3121 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3181 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     3241 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3301 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3361 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3421 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3481 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3541 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3601 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3661 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3721 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3781 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3841 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3901 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3961 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     4021 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4081 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     4141 agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg
     4201 gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
     4261 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
     4321 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
     4381 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
     4441 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
     4501 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
     4561 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
     4621 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
     4681 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
     4741 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
     4801 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
     4861 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
     4921 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
     4981 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
     5041 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
     5101 ctcatgttat atcccgccgt caaccaccat caaacaggat tttcgcctgc tggggcaaac
     5161 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     5221 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
     5281 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5341 gcagtgagcg caacgcaatt aatgtgagtt agcgcgaatt gatctg
//
