LOCUS       40924_43878        5557 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-fumC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5557)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5557)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1667
                     /gene="fumC"
                     /label=fumC
                     /note="Fumarate hydratase class II from Escherichia coli
                     (strain K12). Accession#: P05042"
     terminator      1910..1996
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2088..2115
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2135..2226
                     /label=AmpR promoter
     CDS             2227..3084
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3258..3846
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4032..4172)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4358..4435
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4436..5515
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatga atacagtacg cagcgaaaaa gattcgatgg
      301 gggcgattga tgtcccggca gataagctgt ggggcgcaca aactcaacgc tcgctggagc
      361 atttccgcat ttcgacggag aaaatgccca cctcactgat tcatgcgctg gcgctaacca
      421 agcgtgcagc ggcaaaagtt aatgaagatt taggcttgtt gtctgaagag aaagcgagcg
      481 ccattcgtca ggcggcggat gaagtactgg caggacagca tgacgacgaa ttcccgctgg
      541 ctatctggca gaccggctcc ggcacgcaaa gtaacatgaa catgaacgaa gtgctggcta
      601 accgggccag tgaattactc ggcggtgtgc gcgggatgga acgtaaagtt caccctaacg
      661 acgacgtgaa caaaagccaa agttccaacg atgtctttcc gacggcgatg cacgttgcgg
      721 cgctgctggc gctgcgcaag caactcattc ctcagcttaa aaccctgaca cagacactga
      781 atgagaaatc ccgtgctttt gccgatatcg tcaaaattgg tcgtactcac ttgcaggatg
      841 ccacgccgtt aacgctgggg caggagattt ccggctgggt agcgatgctc gagcataatc
      901 tcaaacatat cgaatacagc ctgcctcacg tagcggaact ggctcttggc ggtacagcgg
      961 tgggtactgg actaaatacc catccggagt atgcgcgtcg cgtagcagat gaactggcag
     1021 tcattacctg tgcacagttt gttaccgcgc cgaacaaatt tgaagcgctg gcgacctgtg
     1081 atgccctggt tcaggcgcac ggcgcgttga aagggttggc tgcgtcactg atgaaaatcg
     1141 ccaatgatgt ccgctggctg gcctctggcc cgcgctgcgg aattggtgaa atctcaatcc
     1201 cggaaaatga gccgggcagc tcaatcatgc cggggaaagt gaatccaaca cagtgtgagg
     1261 cattaaccat gctctgctgt caggtgatgg ggaacgacgt ggcgatcaac atggggggcg
     1321 cttccggtaa ctttgaactg aacgtcttcc gtccaatggt gatccacaat ttcctgcaat
     1381 cggtgcgctt gctggcagat ggcatggaaa gttttaacaa acactgcgca gtgggtattg
     1441 aaccgaatcg tgagcgaatc aatcaattac tcaatgaatc gctgatgctg gtgactgcgc
     1501 ttaacaccca cattggttat gacaaagccg cggagatcgc caaaaaagcg cataaagaag
     1561 ggctgacctt aaaagctgcg gcccttgcgc tggggtatct tagcgaagcc gagtttgaca
     1621 gctgggtacg gccagaacag atggtcggca gtatgaaagc cgggcgttaa tggatcctct
     1681 agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc
     1741 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
     1801 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     1861 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     1921 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1981 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     2041 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     2101 gacggatggc ctttttgcgt ttctacaaac tctttttgtt tatttttcta aatacattca
     2161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2461 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2761 acgatgccta cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2941 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3961 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4021 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4081 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     4141 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     4201 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4261 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca
     4321 attcgcgcgc gaaggcgaag cggcatgcat ttacgttgac accatcgaat ggtgcaaaac
     4381 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
     4441 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
     4501 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
     4561 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
     4621 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
     4681 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
     4741 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
     4801 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
     4861 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
     4921 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
     4981 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
     5041 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
     5101 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
     5161 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     5221 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     5281 tatcccgccg tcaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     5341 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     5401 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     5461 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5521 gcaacgcaat taatgtgagt tagcgcgaat tgatctg
//
