LOCUS       40924_43913        5698 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-SeTAL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5698)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5698)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     5'UTR           247..271
                     /gene="TAL"
     gene            247..271
                     /gene="TAL"
                     /label=TAL
     CDS             272..1804
                     /codon_start=1
                     /gene="TAL"
                     /product="tyrosine ammonia-lyase"
                     /label=TAL
                     /note="derived from Saccharothrix espanaensis"
                     /protein_id="AXN70016.1"
                     /translation="MTQVVERQADRLSSREYLARVVRSAGWDAGLTSCTDEEIVRMGAS
                     ARTIEEYLKSDKPIYGLTQGFGPLVLFDADSELEQGGSLISHLGTGQGAPLAPEVSRLI
                     LWLRIQNMRKGYSAVSPVFWQKLADLWNKGFTPAIPRHGTVSASGDLQPLAHAALAFTG
                     VGEAWTRDADGRWSTVPAVDALAALGAEPFDWPVREALAFVNGTGASLAVAVLNHRSAL
                     RLVRACAVLSARLATLLGANPEHYDVGHGVARGQVGQLTAAEWIRQGLPRGMVRDGSRP
                     LQEPYSLRCAPQVLGAVLDQLDGAGDVLAREVDGCQDNPITYEGELLHGGNFHAMPVGF
                     ASDQIGLAMHMAAYLAERQLGLLVSPVTNGDLPPMLTPRAGRGAGLAGVQISATSFVSR
                     IRQLVFPASLTTLPTNGWNQDHVPMALNGANSVFEALELGWLTVGSLAVGVAQLAAMTG
                     HAAEGVWAELAGICPPLDADRPLGAEVRAARDLLSAHADQLLVDEADGKDFG"
     gene            272..1804
                     /gene="TAL"
                     /label=TAL
     terminator      2051..2137
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2229..2256
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2276..2367
                     /label=AmpR promoter
     CDS             2368..3225
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3399..3987
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4173..4313)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4499..4576
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4577..5656
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaaagac cgaggaaaag gagcatcgca aatgacgcag gtcgtggaac gtcaggctga
      301 tcggctcagc agcagggagt acctggcccg ggtcgtgcgc agcgccgggt gggacgccgg
      361 tctcacctcg tgcaccgacg aggagatcgt ccggatgggc gcgagcgcgc gcaccatcga
      421 ggagtacctg aagtccgaca agcccatcta cggcctgacg cagggcttcg gtccgctggt
      481 gctgttcgac gccgactcgg agctggagca gggcggctcg ctgatctcgc acctgggcac
      541 cggccagggc gcgccactgg ccccggaggt gtcgcggctg atcctctggc tgcgcatcca
      601 gaacatgcgc aaggggtact cggcggtctc gccggtgttc tggcagaagc tcgccgacct
      661 gtggaacaag gggttcaccc cggcgatccc ccggcacggc acggtcagcg cgagcggcga
      721 cctgcaaccg ctggcgcacg ccgcgctcgc cttcaccggt gtcggcgagg cgtggacccg
      781 ggacgccgac ggccggtggt ccaccgtgcc ggccgtggac gcgctcgccg cgctgggggc
      841 ggagccgttc gactggccgg tgcgcgaggc gctggcgttc gtcaacggga ccggcgcgag
      901 cctcgcggtg gctgtgctca accaccggtc cgccctgcgg ctggtccgcg cctgcgccgt
      961 gctctccgcg cggctggcga ccctgctggg ggccaatccc gagcactacg acgtggggca
     1021 cggtgtcgcg cgcggccagg tcggtcagct gaccgcggcg gagtggatcc ggcaggggct
     1081 gccccggggc atggtgcgcg acggcagccg cccgctccag gagccgtaca gcctgcggtg
     1141 cgcgccgcag gtgctcggcg cggtgctcga ccagctcgac ggcgcgggcg acgtgctggc
     1201 gcgggaggtc gacggctgcc aggacaaccc gatcacctac gagggcgagc tgctgcacgg
     1261 cggcaacttc cacgccatgc cggtgggttt cgcctccgac cagatcgggt tggccatgca
     1321 catggccgcc tacctggccg agcgccagct gggtctgctg gtcagcccgg tgaccaacgg
     1381 cgacctgccg cccatgctca ccccgcgcgc cgggcgcggt gccgggctgg ccggggtgca
     1441 gatcagcgcg acctcgttcg tctcgcggat ccggcagctg gtgttccccg cctcgctgac
     1501 caccctgccg accaacggct ggaaccagga ccacgtgccg atggcgctca acggggcgaa
     1561 ctcggtgttc gaggcgttgg agctcggctg gctgacggtc gggtcgctgg cggtgggcgt
     1621 cgcgcagctc gcggccatga ccggccacgc cgcggagggc gtctgggcgg agctggccgg
     1681 gatctgcccg ccgctggacg ccgaccgccc gctgggcgcc gaggtgcgcg ccgcgcgcga
     1741 cctgctgtcc gcgcacgcgg accaactgct cgtcgacgag gcagacggga aggatttcgg
     1801 atgagtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg ctgttttggc
     1861 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     1921 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     1981 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     2041 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2101 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     2161 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     2221 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctctttttgt
     2281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2341 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2401 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2461 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2521 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2581 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     2641 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2701 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2761 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2821 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2881 ccaaacgacg agcgtgacac cacgatgcct acagcaatgg caacaacgtt gcgcaaacta
     2941 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3001 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3061 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3121 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3181 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3241 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3541 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3781 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3841 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3901 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3961 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4021 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4081 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4141 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     4201 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     4261 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     4321 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4381 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4441 aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca tttacgttga
     4501 caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     4561 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     4621 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     4681 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     4741 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     4801 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     4861 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     4921 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     4981 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     5041 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     5101 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     5161 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
     5221 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
     5281 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
     5341 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     5401 taccgaagac agctcatgtt atatcccgcc gtcaaccacc atcaaacagg attttcgcct
     5461 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     5521 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     5581 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5641 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagcgcgaa ttgatctg
//
