LOCUS       40924_43883        5716 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTrc-HisTAL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     5'UTR           247..271
                     /gene="TAL"
     gene            247..271
                     /gene="TAL"
                     /label=TAL
     CDS             275..292
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      2069..2155
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2247..2274
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2294..2385
                     /label=AmpR promoter
     CDS             2386..3243
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3417..4005
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4191..4331)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        4517..4594
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4595..5674
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaaagac cgaggaaaag gagcatcgca aatgcaccat catcatcatc atacgcaggt
      301 cgtggaacgt caggctgatc ggctcagcag cagggagtac ctggcccggg tcgtgcgcag
      361 cgccgggtgg gacgccggtc tcacctcgtg caccgacgag gagatcgtcc ggatgggcgc
      421 gagcgcgcgc accatcgagg agtacctgaa gtccgacaag cccatctacg gcctgacgca
      481 gggcttcggt ccgctggtgc tgttcgacgc cgactcggag ctggagcagg gcggctcgct
      541 gatctcgcac ctgggcaccg gccagggcgc gccactggcc ccggaggtgt cgcggctgat
      601 cctctggctg cgcatccaga acatgcgcaa ggggtactcg gcggtctcgc cggtgttctg
      661 gcagaagctc gccgacctgt ggaacaaggg gttcaccccg gcgatccccc ggcacggcac
      721 ggtcagcgcg agcggcgacc tgcaaccgct ggcgcacgcc gcgctcgcct tcaccggtgt
      781 cggcgaggcg tggacccggg acgccgacgg ccggtggtcc accgtgccgg ccgtggacgc
      841 gctcgccgcg ctgggggcgg agccgttcga ctggccggtg cgcgaggcgc tggcgttcgt
      901 caacgggacc ggcgcgagcc tcgcggtggc tgtgctcaac caccggtccg ccctgcggct
      961 ggtccgcgcc tgcgccgtgc tctccgcgcg gctggcgacc ctgctggggg ccaatcccga
     1021 gcactacgac gtggggcacg gtgtcgcgcg cggccaggtc ggtcagctga ccgcggcgga
     1081 gtggatccgg caggggctgc cccggggcat ggtgcgcgac ggcagccgcc cgctccagga
     1141 gccgtacagc ctgcggtgcg cgccgcaggt gctcggcgcg gtgctcgacc agctcgacgg
     1201 cgcgggcgac gtgctggcgc gggaggtcga cggctgccag gacaacccga tcacctacga
     1261 gggcgagctg ctgcacggcg gcaacttcca cgccatgccg gtgggtttcg cctccgacca
     1321 gatcgggttg gccatgcaca tggccgccta cctggccgag cgccagctgg gtctgctggt
     1381 cagcccggtg accaacggcg acctgccgcc catgctcacc ccgcgcgccg ggcgcggtgc
     1441 cgggctggcc ggggtgcaga tcagcgcgac ctcgttcgtc tcgcggatcc ggcagctggt
     1501 gttccccgcc tcgctgacca ccctgccgac caacggctgg aaccaggacc acgtgccgat
     1561 ggcgctcaac ggggcgaact cggtgttcga ggcgttggag ctcggctggc tgacggtcgg
     1621 gtcgctggcg gtgggcgtcg cgcagctcgc ggccatgacc ggccacgccg cggagggcgt
     1681 ctgggcggag ctggccggga tctgcccgcc gctggacgcc gaccgcccgc tgggcgccga
     1741 ggtgcgcgcc gcgcgcgacc tgctgtccgc gcacgcggac caactgctcg tcgacgaggc
     1801 agacgggaag gatttcggat gagtacccgg ggatcctcta gagtcgacct gcaggcatgc
     1861 aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
     1921 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     1981 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     2041 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2101 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     2161 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     2221 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     2281 tctacaaact ctttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2341 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2401 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2461 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2521 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2581 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     2641 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2701 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2761 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2821 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2881 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctac agcaatggca
     2941 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3001 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3061 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3121 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3181 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3241 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3301 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3361 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3421 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3481 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3541 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3601 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3661 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3721 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3781 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3841 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3901 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3961 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4021 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4081 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4141 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     4201 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     4261 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     4321 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4381 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4441 tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc
     4501 ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata
     4561 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
     4621 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
     4681 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
     4741 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
     4801 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
     4861 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
     4921 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
     4981 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
     5041 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
     5101 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
     5161 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
     5221 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
     5281 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
     5341 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
     5401 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt caaccaccat
     5461 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     5521 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     5581 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5641 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5701 agcgcgaatt gatctg
//
