LOCUS 40924_43803 3182 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pToxin-ecoRI, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3182) AUTHORS Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. TITLE 'Deadman' and 'Passcode' microbial kill switches for bacterial containment JOURNAL Nat. Chem. Biol. 12 (2), 82-86 (2016) PUBMED 26641934 REFERENCE 2 (bases 1 to 3182) AUTHORS Chan CTY., Lee JW, Cameron DE, Bashor CJ, Collins JJ. TITLE Direct Submission JOURNAL Submitted (12-OCT-2015) Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., E25-302b, Cambridge, MA 02139, USA REFERENCE 3 (bases 1 to 3182) TITLE Direct Submission REFERENCE 4 (bases 1 to 3182) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. Biol."; date: "2016"; volume: "12"; issue: "2"; pages: "82-86" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (12-OCT-2015) Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., E25-302b, Cambridge, MA 02139, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3182 /mol_type="other DNA" /organism="synthetic DNA construct" terminator 14..108 /label=lambda t0 terminator /note="transcription terminator from phage lambda" regulatory 115..189 /label=T0 /note="T0" /regulatory_class="promoter" regulatory 195..214 /label=RBS /note="RBS" /regulatory_class="ribosome_binding_site" CDS 221..1051 /gene="ecoRIR" /label=ecoRIR /note="Type II restriction enzyme EcoRI from Escherichia coli. Accession#: P00642" terminator 1079..1165 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 1286..1831 /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." terminator complement(1993..2087) /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2120..2977) /label=AmpR /note="beta-lactamase" promoter complement(2978..3082) /label=AmpR promoter ORIGIN 1 ctcgagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca 61 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtccg gccgcattta 121 ttaaaccggt ttattgacat aaaccggttt agcatagata ctgagcacat cagcaggacg 181 cactgaccca attgctcaca accacgaagg aacaggatcc atgtctaata aaaaacagtc 241 aaataggcta actgaacaac ataagttatc tcaaggtgta attgggattt ttggggatta 301 tgcaaaagct catgatctcg ctgttggtga ggtttcaaaa ttagtaaaga aagctcttag 361 caacgaatac cctcaattat catttcgata tagagatagt ataaagaaaa cagaaataaa 421 tgaggcttta aaaaaaattg accctgatct tggcggtact ttatttgttt caaattccag 481 catcaaacct gatggtggaa ttgtagaggt caaagatgat tatggtgaat ggagagttgt 541 acttgttgct gaagccaaac accaaggtaa agatattata aatataagga atggtttgtt 601 agttgggaaa agaggagatc aagatttaat ggctgctggt aatgctatcg aaagatctca 661 taagaatata tcagagatag cgaattttat gctctctgag agccactttc cttacgtcct 721 tttcttagag gggtctaact ttttaacaga aaatatctca ataacaagac cagatggaag 781 ggttgttaat cttgagtata attctggtat attaaatagg ttagatcgac taacagcggc 841 taattatgga atgcctataa atagtaatct atgtattaac aaatttgtaa atcataaaga 901 caaaagcatt atgctacaag cagcatctat atatactcaa ggagatggga gggagtggga 961 ttcgaaaatc atgtttgaaa taatgtttga tatatcaacg acttcgctca gagtgttggg 1021 gcgtgacttg tttgaacagc ttacatctaa gtgaccatgg tacgcgtgct agaggcatca 1081 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1141 gaacgctctc ctgagtagga caaatccgcc gccctagacc tagtaacaac ttatatcgta 1201 tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt 1261 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct 1321 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact 1381 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt 1441 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct 1501 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag 1561 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc 1621 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac 1681 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc 1741 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg 1801 atgcttgtca ngggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc 1861 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc 1921 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc 1981 cctagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa 2041 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcgt 2101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 2161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 2221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 2281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 2341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 2401 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 2461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 2521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 2581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 2641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 2701 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 2761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 2821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 2881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 2941 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 3001 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 3061 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 3121 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc 3181 ac //