LOCUS       V002567                 3182 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002567
VERSION     V002567
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ.
  TITLE     'Deadman' and 'Passcode' microbial kill switches for bacterial
            containment
  JOURNAL   Nat. Chem. Biol. 12 (2), 82-86 (2016)
   PUBMED   26641934
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Chan CTY., Lee JW, Cameron DE, Bashor CJ, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2015) Department of Biological Engineering,
            Massachusetts Institute of Technology, 77 Massachusetts Ave.,
            E25-302b, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3182)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3182)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2016"; volume: "12"; issue: "2"; pages: "82-86"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2015) Department of Biological Engineering, Massachusetts
            Institute of Technology, 77 Massachusetts Ave., E25-302b, Cambridge,
            MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..108
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     regulatory      115..189
                     /label="T0"
                     /note="T0"
                     /regulatory_class="promoter"
     regulatory      195..214
                     /label="RBS"
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             221..1051
                     /gene="ecoRIR"
                     /label="Type II restriction enzyme EcoRI"
                     /note="Type II restriction enzyme EcoRI from Escherichia
                     coli. Accession#: P00642"
     terminator      1079..1165
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1286..1831
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1993..2087)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2120..2977)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2978..3082)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
       61 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtccg gccgcattta
      121 ttaaaccggt ttattgacat aaaccggttt agcatagata ctgagcacat cagcaggacg
      181 cactgaccca attgctcaca accacgaagg aacaggatcc atgtctaata aaaaacagtc
      241 aaataggcta actgaacaac ataagttatc tcaaggtgta attgggattt ttggggatta
      301 tgcaaaagct catgatctcg ctgttggtga ggtttcaaaa ttagtaaaga aagctcttag
      361 caacgaatac cctcaattat catttcgata tagagatagt ataaagaaaa cagaaataaa
      421 tgaggcttta aaaaaaattg accctgatct tggcggtact ttatttgttt caaattccag
      481 catcaaacct gatggtggaa ttgtagaggt caaagatgat tatggtgaat ggagagttgt
      541 acttgttgct gaagccaaac accaaggtaa agatattata aatataagga atggtttgtt
      601 agttgggaaa agaggagatc aagatttaat ggctgctggt aatgctatcg aaagatctca
      661 taagaatata tcagagatag cgaattttat gctctctgag agccactttc cttacgtcct
      721 tttcttagag gggtctaact ttttaacaga aaatatctca ataacaagac cagatggaag
      781 ggttgttaat cttgagtata attctggtat attaaatagg ttagatcgac taacagcggc
      841 taattatgga atgcctataa atagtaatct atgtattaac aaatttgtaa atcataaaga
      901 caaaagcatt atgctacaag cagcatctat atatactcaa ggagatggga gggagtggga
      961 ttcgaaaatc atgtttgaaa taatgtttga tatatcaacg acttcgctca gagtgttggg
     1021 gcgtgacttg tttgaacagc ttacatctaa gtgaccatgg tacgcgtgct agaggcatca
     1081 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1141 gaacgctctc ctgagtagga caaatccgcc gccctagacc tagtaacaac ttatatcgta
     1201 tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt
     1261 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
     1321 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
     1381 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
     1441 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
     1501 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
     1561 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     1621 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     1681 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc
     1741 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     1801 atgcttgtca ngggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     1861 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     1921 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     1981 cctagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     2041 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcgt
     2101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2401 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2701 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2941 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3001 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3061 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3121 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     3181 ac
//
