LOCUS       .                       9227 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4550..5266
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    complement(6887..7008)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7680..7696)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(7733..7751)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7772..7788)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7796..7812)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7820..7850)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7865..7886)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8174..8762)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag accagcaaag ttctagaatt
     1081 tgtcgaaaca tttatgttat atatttcctg aaaaaaattc tgagtaagtt cttaagtgtt
     1141 attgccagca acataaacaa cagacggcaa aatgaataaa tgataacaaa gcagtaggct
     1201 taaataaacc taatttttat aggctgttct ctacaaccct caaacagtga ttagttttgt
     1261 acttataaac ttgccctttc attcatattt caagaaaatt ggttcagaag atctggatat
     1321 tctagcagtt gttcaagctc atggagggat cagtgacctg attccacaat gactaggcct
     1381 aatccagaaa ttagatgact gtcaacataa aaaggcacag cactcactag ctgccctata
     1441 tattttatta tattttacat atattatttt atttatttag ctctgagtgc tgtactttct
     1501 ggttaaagaa aactgcttac aacagctaac ctgtactacc tcaggctcag ggaatttgga
     1561 acaggtttgt ctggtttgtt tctttaacca tgcatgcttg ttttcaacta tggcaacaca
     1621 gtcacatggg acattacaga aatgatttgt cgatgacatg cgacttttct ttaataaagc
     1681 gcaaagatcc caaaaagcaa acttttaaca aaaatcatat aattatattt tcaatccagc
     1741 tttgtagcaa ctttgtgctg ctgttcactc agcaacagat agtcagtata aggtcagtgt
     1801 gtctcaaagc agtgccatct gtttcacaca ttgcgttcta tatataagtg tgctggttga
     1861 cacgacactg tataaggcct aggctaaaac acaaacaatg tagaatgaca ctgtgttttt
     1921 tttgtaaaca aatgttgttt ttggttaaac atctttgtga aaacatcctc ctgtcatgta
     1981 tttgctatat tcaaatgtta aacccgtgca gaatagaaca tatacaaaaa aaaacaacac
     2041 aacacatttt taaacattat taaatatcaa gtattgctgg cagttctgtt tctgttttac
     2101 agtacccttt gccacagttc tccgcttttc ctggtccaga ttccacaagt ctgattcacc
     2161 aatagcaaag cgaataaaca accaaagcag ccaatcactg cttgtagact gtcctgcgag
     2221 accggcccat tccagcacat tctggaaact tcctttatat gataattata aatacattta
     2281 aattattgat acaaaacatg taattcctag aacataacca tagcaatcat tagttttcag
     2341 ggtaattatg tatttttagg atttgactgc ggaaagatct ggtcatgtga cgtctcatga
     2401 acgtcacggc cctgggtttc tataaataca gtaggactct cgaccatcgg cagatttttc
     2461 gaagaagaag atcagtttca ggagccgtac tgttccgttt caacgcaaat attaacggta
     2521 agagcgaatt tcctagtttg ttttcatgcc attctttaaa accatagcgt attactttaa
     2581 ttatagtaaa ctttcgcttt ctttattaca agagacgttt tgtgttgatt ctccgcggac
     2641 attttcggtc agacaatcag aaaatgaccg cggaggacca gtaacttgca ttacacgtaa
     2701 gttaaatctt cgtgtattaa aatggttagg ttgttaacgt caaataggtt accgtgtttg
     2761 cgtgtgatca ggttggtttt gttagatttt tgtcagtatt tttaatttat ttgttttagt
     2821 ttatttattt tttttgctga atcatagttt gtgaacaaag aacccggatg ttacatacag
     2881 tacagccgcc atgttacaga gagttataac ttaatcattt taaaaataat tttgccttac
     2941 ttttagtttg tcatgttgag aaatgaggaa atgttaaaat gaggaaatat ccaattaatt
     3001 taatatatca aaataatcca tgattacaat gcactgaact ggagaaaatt aagatgtttt
     3061 ctagtgtcat gaaacaaatg taagagatgt acattgtaga tgttttatgt caagaattgg
     3121 ctagttgatg cagcatactg gcgatactca gttgtaataa cagtaacgtt acatgttaat
     3181 agactactga gtatgctgtt ctgtctatgt atgctctgta agctacgaga aggacttttt
     3241 taaacagtaa agggtgcaat atttttacaa attgaattaa ataaaggctg tctattaagt
     3301 aatatgcttg atatttttct tacttgatcg gaaataagaa aaaatataaa cgttgttgct
     3361 ctaaaaatcc tagttcagtt tagccaacca caaatacctt tttgttcctc caacagtttt
     3421 tttttcttct ctataatatt tggcagtcta tagtactcca aatgtttccc cacagtctaa
     3481 ctaattggta cagccaaaat catgacactt attgcaataa taattttggt tcattggcat
     3541 tgttgatagc ctgtgccact aatatggtcg attgatcatg cttcaggaag aaaactatat
     3601 tgtttgatgt aagattatta aatcttcacc tgcttccatt acaaactatt cccatcttat
     3661 tgaattctgg tatgtcttaa aggattagtt cacttcccaa atcaaaattt acttagcctt
     3721 ttttcatcca tgatcccttt ttttcatcat taatgaagaa attgtttttg aaaaagtttc
     3781 aagatttttt tctctatatt gtggagcttg ttagtttaaa attccaaaat gcaatatgtg
     3841 gcttcaaatg gttctaaatg atcccagtca aggaataaca gtcttatcta atgaaaccat
     3901 tagaccttta aaaataaaaa aaataaaagt atttattttt aaatgactga gtgattaagt
     3961 tgaatttcag cgtttcctta ctgtgtagaa gtccttcctt actggcccca ccctttggtt
     4021 ctctgccaat ctgctaccta atgtaatgtt gtggaacatt attattcttt atttcttaat
     4081 tttttatttt ttattttaaa acaatgtaaa ctgcacagat gtgcagtttg tttaaaatgg
     4141 ccaatgcttt ggaaatgcat gacataatta gatttcatga tgcacaaagc caaatctcag
     4201 agcttgtgca aaatgagcta tcatttcact aggtaagacc ctaaattttc atataggatc
     4261 atttggacaa ttttgctgca ggtaaaatgc attctatagt ccactgtcag ccattgtttt
     4321 ggatagtatt tatttttctc tacaagtata gtcaatagtt ttctattatt ttaaaggttt
     4381 gtaacattta agggtgacca aatgcaaagt aaaatttcat tttcgggtga actatctcgt
     4441 ttaacatggg agaagtgcaa aacatacatt attggctaga acattgtagt attttttaaa
     4501 tggaaatgtg tgattgctaa tcttactttg aatttgttta cagggatcca tggtgagcaa
     4561 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4621 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4681 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4741 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4801 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4861 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4921 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4981 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5041 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5101 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5161 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5221 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggaa gcggagctac
     5281 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctcccgggat
     5341 gtcgacgatg gactacagtt atgatgcgga cctggaagag atgtgtccgg tgtgcgggga
     5401 taaagtttct ggctatcatt atggtttatt aacctgcgaa agctgcaagg gtttctttaa
     5461 aaggaccgta cagaataaca aacggtacac atgtgcagag agtcaggact gtaagattga
     5521 taaaacccag aggaaacggt gtcctttctg ccgcttccag aaatgtctca acgtcgggat
     5581 gcgactggaa gctgtccgtg cggataggat gcgaggaggc cgcaacaaat tcggccccat
     5641 gtataagcga gaccgggcgt taaagcagca gaagaaagct ctcatccggg ccagtggctt
     5701 aaagatggaa gccacacccc ctttgctgac gtcaccacaa cctgactaca ccttcagcac
     5761 agctttgtct gtgccagctc cgaaaaacac ccatccgaac atcgtcacct ctgtggcacc
     5821 caccgattac gaacgcagcc tctacgcatc cagctccctg agtttatccg tccccatccc
     5881 ggcccacact cctctcccag cacaataccc ctatccaaat ctgcccagcc gtgccataaa
     5941 gtctgagtat cctgaccact acacaagctc agagcattac accagtgcca gctccccaga
     6001 gtcagtgcca ggctacacgt atatcgagca aacacgggtg tccagcagcc cgcaggtggt
     6061 ggcaccgggt ttaacagtgc cgcctttggt tctggagttt gttcgctgtg agcaggatga
     6121 gctccaggtg caaagcaaaa taagcgctca cctggcgcac ctgcagcagg agcagaactc
     6181 acggagcacg gcagccaatc aggagcagag ctcccgcctc gcagccaaac ccgagcggct
     6241 cagcacattt ggcctcatgt gccacatggc cgaccagacg ctcttctcta tcgtggagtg
     6301 ggcacgaagc tgcatcttct ttaaggagct taaggttgga gatcagatga agttgctgca
     6361 taactgctgg agtgaacttc tagtgctgga ctatgttgcc agacagttgc atcatgggaa
     6421 agaggacagt gtgcttctca ttactggaca ggaggtggag cttgcatctc tcctggccca
     6481 ggcaggggtc actttgagcg ggatgatcca gagaggacag gagctggtgc agcgtctaca
     6541 ggagctacag ctggaccgca gagaaaccgc ctgcctcaaa tacctcatcc tctttaaccc
     6601 cgatgtgaaa ctgttggaaa accagcctta cgtggagagc gtgtacgagc aggtgaacgc
     6661 cgcgctgttg gagtacacgc tgtgcgcgta tccgcaattc ccggacaggt ttagccagat
     6721 tctgctgcgc cttcctgagc tcagagcgct gagttcgcaa gccgaagact acctgtgcta
     6781 caaacacctg agcggagagg tgccctgcaa caacctcctc atagaaatgc tgcatgccaa
     6841 gagaacctgc atctgacata tgtaagtata cttgatatcg acatgataag atacattgat
     6901 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     6961 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     7021 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     7081 aacctctaca aatgtggtat ggctgattat gatcctctag atcagatctg cgaagatacg
     7141 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     7201 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     7261 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     7321 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     7381 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     7441 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     7501 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     7561 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     7621 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     7681 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     7741 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7801 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7861 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7921 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7981 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8341 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8821 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8881 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8941 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9001 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9061 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9121 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9181 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttg
//
