LOCUS       62056_21250        7646 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3385..4101
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4227..4348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4562..5017)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5044..5148
                     /label=AmpR promoter
     promoter        5150..5507
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5542..6333
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6568..6615
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6944..7532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta
       61 ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta
      121 cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt
      181 caaaaagtac ttattttttg gagatcactt cattctattt tcccttgcta ttagctagcg
      241 ctaccggact cagatctcga gcgtttactc tgagaaactt ccatatttta gaggtaaaat
      301 gctcagaatt gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag
      361 tcacatcacc tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg
      421 tttataactc ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca
      481 tgtagtttat catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa
      541 tatctgaaat gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt
      601 tttttttcta cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa
      661 aaagaaatgt tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt
      721 taagatcaag acttgacata taacatttct aatgaacatt aaacctgagg taggcccact
      781 tgcaaaaatg taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa
      841 aatagtgaag caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact
      901 ttcttaaagt gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa
      961 gtagtgtcat cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat
     1021 tagatccccc attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag
     1081 agctgctgtt gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg
     1141 acgcattctg ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc
     1201 gttgtcgcag aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt
     1261 tataggaaat cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc
     1321 aagttgcaag aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc
     1381 aatcagagca cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc
     1441 atataaaaga gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt
     1501 gcggaatatc atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt
     1561 gagttggttt tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa
     1621 aatgtgtaaa tggataaata tgatttgatg aagtctttag acagttcata acggtctctt
     1681 tgtagttatc tactaggttt gatttataag tggtggttct tttgataatc tatgttacat
     1741 ttaataaata ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga
     1801 atgtcggtaa tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg
     1861 tgaaatgggt gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc
     1921 ataaatgtga tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg
     1981 gatagccggc atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt
     2041 agctagccgg ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg
     2101 ctcattcgtg ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt
     2161 tttaacttgg ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc
     2221 tctgtcttta atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg
     2281 aaagatttga attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg
     2341 cacttcagcc gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt
     2401 tttgaggcta gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt
     2461 gaagtctccg ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc
     2521 ggcggcagac acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg
     2581 atgtgaaact tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat
     2641 ctgttccttt ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa
     2701 agcggctcaa accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg
     2761 tctggcatat ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg
     2821 cagagcaaaa caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg
     2881 gcagcagtgc aattctaaca gtttcccttt cttttacagt tcagccgaat tctgcagtcg
     2941 acggtaccag ccaccatggg attcaaaacg tgtggttttt caaaaaaatg gttagtaaca
     3001 gcagttatag ttatgtgttt gtgtaccgag tgttattgcc aatgcactgg aggtgctgat
     3061 tgtactagtt gtacagcagc atgcactggt tgtggaaact gtccaaatgc agtaacgtgt
     3121 accaattctc aacattgtgt caaggcaaca acatgtactg ggtctacaga ttgtaataca
     3181 gccgtgacgt gtacaaactc aaaagactgt ttcgaagccc aaacatgtac tgactcaacc
     3241 aactgttaca aagctacagc ctgtaccaat tcaacaggat gtcccggaca tggatccgga
     3301 ggtggcagcg gtggcggtca gctgttgaat tttgaccttc ttaaacttgc gggagacgtc
     3361 gagtccaacc ctgggcccac aaccatggtg agcaagggcg aggagctgtt caccggggtg
     3421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3481 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3661 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3781 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3841 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3901 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3961 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     4021 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4081 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     4141 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4201 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     4261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4321 gtttgtccaa actcatcaat gtatcttaag gcttaaacaa gaatctctag ttttctttct
     4381 tgcttttact tttacttcct taatactcaa gtacaatttt aatggagtac ttttttactt
     4441 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
     4501 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct gcttaaggcg
     4561 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4621 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4681 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4741 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     4801 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     4861 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     4921 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     4981 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     5041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     5161 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5461 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     5521 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     5581 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5641 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5701 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     5761 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5821 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5881 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5941 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6001 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6061 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6121 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6181 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6241 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6301 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     6361 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6421 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6481 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     6541 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     6601 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     6661 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     6721 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     6781 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7081 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7561 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7621 tggataaccg tattaccgcc atgcat
//
