LOCUS       62056_21245        7769 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3508..4224
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4350..4471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4685..5140)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5167..5271
                     /label=AmpR promoter
     promoter        5273..5630
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5665..6456
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6691..6738
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7067..7655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta
       61 ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta
      121 cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt
      181 caaaaagtac ttattttttg gagatcactt cattctattt tcccttgcta ttagctagcg
      241 ctaccggact cagatctcga gcgtttactc tgagaaactt ccatatttta gaggtaaaat
      301 gctcagaatt gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag
      361 tcacatcacc tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg
      421 tttataactc ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca
      481 tgtagtttat catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa
      541 tatctgaaat gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt
      601 tttttttcta cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa
      661 aaagaaatgt tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt
      721 taagatcaag acttgacata taacatttct aatgaacatt aaacctgagg taggcccact
      781 tgcaaaaatg taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa
      841 aatagtgaag caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact
      901 ttcttaaagt gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa
      961 gtagtgtcat cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat
     1021 tagatccccc attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag
     1081 agctgctgtt gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg
     1141 acgcattctg ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc
     1201 gttgtcgcag aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt
     1261 tataggaaat cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc
     1321 aagttgcaag aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc
     1381 aatcagagca cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc
     1441 atataaaaga gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt
     1501 gcggaatatc atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt
     1561 gagttggttt tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa
     1621 aatgtgtaaa tggataaata tgatttgatg aagtctttag acagttcata acggtctctt
     1681 tgtagttatc tactaggttt gatttataag tggtggttct tttgataatc tatgttacat
     1741 ttaataaata ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga
     1801 atgtcggtaa tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg
     1861 tgaaatgggt gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc
     1921 ataaatgtga tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg
     1981 gatagccggc atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt
     2041 agctagccgg ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg
     2101 ctcattcgtg ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt
     2161 tttaacttgg ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc
     2221 tctgtcttta atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg
     2281 aaagatttga attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg
     2341 cacttcagcc gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt
     2401 tttgaggcta gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt
     2461 gaagtctccg ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc
     2521 ggcggcagac acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg
     2581 atgtgaaact tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat
     2641 ctgttccttt ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa
     2701 agcggctcaa accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg
     2761 tctggcatat ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg
     2821 cagagcaaaa caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg
     2881 gcagcagtgc aattctaaca gtttcccttt cttttacagt tcagccgaat tctgcagtcg
     2941 acggtaccag ccaccatgaa gtcagctatt ttaactggtt tgcttttcgt cctcctttgt
     3001 gtcgaccaca tgagttcagc ccaccaggtg agatattctt gctccacaaa taaatattca
     3061 aagatgtgag ccacagtaaa attcaattgt tttctgttta gaaagacaga gaacctttaa
     3121 gaagacattt ttgaaatagt tttttcaact gtgccatgag aacaataata atgtctgacc
     3181 ttttattttc cattcttcaa ggcgtccgtg gtggccaccc agctgatccc cataaatact
     3241 gccctgactc tggtgatgat gacgaccagg gtggtcttcc caacgggcat ccccgccgag
     3301 gacattcccc gattagtctc aatgcaagtg aacagggcag tgccgatggg cacaaccctc
     3361 atgccagaca tggtgaaatt ttactgcctc tgcgcgccga agacccaaag atggggatcc
     3421 ggaggtggca gcggtggcgg tcagctgttg aattttgacc ttcttaaact tgcgggagac
     3481 gtcgagtcca accctgggcc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg
     3541 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3601 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3661 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3721 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3781 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3841 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3901 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3961 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4021 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4081 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4141 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4201 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     4261 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     4321 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4381 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4441 gtggtttgtc caaactcatc aatgtatctt aaggcttaaa caagaatctc tagttttctt
     4501 tcttgctttt acttttactt ccttaatact caagtacaat tttaatggag tactttttta
     4561 cttttactca agtaagattc tagccagata cttttacttt taattgagta aaattttccc
     4621 taagtacttg tactttcact tgagtaaaat ttttgagtac tttttacacc tctgcttaag
     4681 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4741 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4801 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4861 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4921 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4981 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5041 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5101 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     5161 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5221 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     5281 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5341 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5401 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5461 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5521 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5581 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     5641 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5701 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5761 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5821 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     5881 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5941 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6001 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6061 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6121 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6181 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6241 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6301 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6361 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6421 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6481 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6541 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6601 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     6661 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     6721 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     6781 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     6841 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     6901 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6961 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7201 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7441 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7681 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7741 ctgtggataa ccgtattacc gccatgcat
//
