LOCUS       62056_21235        7312 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3051..3767
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3893..4014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4228..4683)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4710..4814
                     /label=AmpR promoter
     promoter        4816..5173
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5208..5999
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6234..6281
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6610..7198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta
       61 ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta
      121 cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt
      181 caaaaagtac ttattttttg gagatcactt cattctattt tcccttgcta ttagctagcg
      241 ctaccggact cagatctcga gcgtttactc tgagaaactt ccatatttta gaggtaaaat
      301 gctcagaatt gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag
      361 tcacatcacc tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg
      421 tttataactc ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca
      481 tgtagtttat catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa
      541 tatctgaaat gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt
      601 tttttttcta cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa
      661 aaagaaatgt tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt
      721 taagatcaag acttgacata taacatttct aatgaacatt aaacctgagg taggcccact
      781 tgcaaaaatg taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa
      841 aatagtgaag caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact
      901 ttcttaaagt gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa
      961 gtagtgtcat cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat
     1021 tagatccccc attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag
     1081 agctgctgtt gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg
     1141 acgcattctg ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc
     1201 gttgtcgcag aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt
     1261 tataggaaat cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc
     1321 aagttgcaag aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc
     1381 aatcagagca cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc
     1441 atataaaaga gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt
     1501 gcggaatatc atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt
     1561 gagttggttt tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa
     1621 aatgtgtaaa tggataaata tgatttgatg aagtctttag acagttcata acggtctctt
     1681 tgtagttatc tactaggttt gatttataag tggtggttct tttgataatc tatgttacat
     1741 ttaataaata ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga
     1801 atgtcggtaa tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg
     1861 tgaaatgggt gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc
     1921 ataaatgtga tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg
     1981 gatagccggc atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt
     2041 agctagccgg ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg
     2101 ctcattcgtg ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt
     2161 tttaacttgg ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc
     2221 tctgtcttta atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg
     2281 aaagatttga attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg
     2341 cacttcagcc gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt
     2401 tttgaggcta gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt
     2461 gaagtctccg ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc
     2521 ggcggcagac acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg
     2581 atgtgaaact tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat
     2641 ctgttccttt ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa
     2701 agcggctcaa accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg
     2761 tctggcatat ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg
     2821 cagagcaaaa caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg
     2881 gcagcagtgc aattctaaca gtttcccttt cttttacagt tcagccgaat tctgcagtcg
     2941 acggtaccgc gggcccggga tccggaggtg gcagcggtgg cggtcagctg ttgaattttg
     3001 accttcttaa acttgcggga gacgtcgagt ccaaccctgg gcccacaacc atggtgagca
     3061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     3301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     3781 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3841 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggctt
     4021 aaacaagaat ctctagtttt ctttcttgct tttactttta cttccttaat actcaagtac
     4081 aattttaatg gagtactttt ttacttttac tcaagtaaga ttctagccag atacttttac
     4141 ttttaattga gtaaaatttt ccctaagtac ttgtactttc acttgagtaa aatttttgag
     4201 tactttttac acctctgctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     4261 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     4321 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     4381 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     4441 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     4501 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     4561 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     4621 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4681 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4741 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4801 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4921 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4981 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5041 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5101 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5161 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5221 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5281 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5341 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     5401 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5461 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5521 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5581 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5641 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5701 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     5761 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5821 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5881 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5941 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6001 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6061 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6121 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     6181 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     6241 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     6301 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     6361 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     6421 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     6481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6721 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7261 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
