LOCUS       40924_43728       10022 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter capture vector pTn7xluxK3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux : A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 10022)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    1292..1316
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1347..1812
                     /label=PthiC
                     /note="PthiC"
                     /regulatory_class="promoter"
     CDS             1839..3278
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             3293..4213
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             4265..5344
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             5362..6342
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             6524..7633
                     /label=LuxE
                     /note="LuxE"
     protein_bind    complement(7671..7695)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8125..8916
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(9018..9065)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(9111..9197)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9300..9394)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9422..9438)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9443..9641
                     /label=Tn7R
                     /note="Tn7R"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg
      181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg
      241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
      301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
      361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
      421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct
      481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc
      541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa
      601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
      661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
      721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
      781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
      841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg
      961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga
     1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     1261 tggcctgcaa ggccttcgcg aggtacccat cacaagtttg tacaaaaaag caggctttaa
     1321 ttaatcgagg cgcgccatcg cactatgtga tcgatggctc tcctgggtga ttgtcgggag
     1381 aacctggcac gcggggccac cgggactggt ggcgagaagg acgcgccggc aaggcaggca
     1441 tggcgcggac atcttgttcc ctacgcgggt tgctgcggtc cgcgcgaggc ggaaccggac
     1501 gctagcccga tcaggttcaa cgggatccgg aactatccga tctcagcccc tcgtataggg
     1561 caccccgaca agaaccccgg cagtctaatc aacaccggcg ggatacgcca acgggaaaac
     1621 gccccgcccg accggcagtc atggtccagt ccggcgccct ctcgccaggg aagacgcaga
     1681 tccccgcacg ttcgccggca catcgatccg gactggctct tcgatcagtt gccgcgcagg
     1741 tgccttgacc gccaccggta gcgaccttag ccttgcccca cctcgcatct tttccaggac
     1801 gaacgacacg cggtgcatta aatggatggc aaatatgaat gactaaaaaa atttcattca
     1861 ttattaacgg ccaggttgaa atctttcccg aaagtgatga tttagtgcaa tccattaatt
     1921 ttggtgataa tagtgtttac ctgccaatat tgaatgactc tcatgtaaaa aacattattg
     1981 attgtaatgg aaataacgaa ttacggttgc ataacattgt caattttctc tatacggtag
     2041 ggcaaagatg gaaaaatgaa gaatactcaa gacgcaggac atacattcgt gacttaaaaa
     2101 aatatatggg atattcagaa gaaatggcta agctagaggc caattggata tctatgattt
     2161 tatgttctaa aggcggcctt tatgatgttg tagaaaatga acttggttct cgccatatca
     2221 tggatgaatg gctacctcag gatgaaagtt atgttcgggc ttttccgaaa ggtaaatctg
     2281 tacatctgtt ggcaggtaat gttccattat ctgggatcat gtctatatta cgcgcaattt
     2341 taactaagaa tcagtgtatt ataaaaacat cgtcaaccga tccttttacc gctaatgcat
     2401 tagcgttaag ttttattgat gtagacccta atcatccgat aacgcgctct ttatctgtta
     2461 tatattggcc ccaccaaggt gatacatcac tcgcaaaaga aattatgcga catgcggatg
     2521 ttattgtcgc ttggggaggg ccagatgcga ttaattgggc ggtagagcac gcgccatctt
     2581 atgctgatgt gattaaattt ggttctaaaa agagtctttg cattatcgat aatcctgttg
     2641 atttgacgtc cgcagcgaca ggtgcggctc atgatgtttg tttttacgat cagcgagctt
     2701 gtttttctgc ccaaaacata tattacatgg gaaatcatta tgaggaattt aagttagcgt
     2761 tgatagaaaa acttaatcta tatgcgcata tattaccgaa tgccaaaaaa gattttgatg
     2821 aaaaggcggc ctattcttta gttcaaaaag aaagcctgtt tgctggatta aaagtagagg
     2881 tggatattca tcaacgttgg atgattattg agtcaaatgc aggtgtggaa tttaatcaac
     2941 cacttggcag atgtgtgtac cttcatcacg tcgataatat tgagcaaata ttgccttatg
     3001 ttcaaaaaaa taagacgcaa accatatcta tttttccttg ggagtcatca tttaaatatc
     3061 gagatgcgtt agcattaaaa ggtgcggaaa ggattgtaga agcaggaatg aataacatat
     3121 ttcgagttgg tggatctcat gacggaatga gaccgttgca acgattagtg acatatattt
     3181 ctcatgaaag gccatctaac tatacggcta aggatgttgc ggttgaaata gaacagactc
     3241 gattcctgga agaagataag ttccttgtat ttgtcccata ataggtaaaa gtatggaaaa
     3301 tgaatcaaaa tataaaacca tcgaccacgt tatttgtgtt gaaggaaata aaaaaattca
     3361 tgtttgggaa acgctgccag aagaaaacag cccaaagaga aagaatgcca ttattattgc
     3421 gtctggtttt gcccgcagga tggatcattt tgctggtctg gcggaatatt tatcgcggaa
     3481 tggatttcat gtgatccgct atgattcgct tcaccacgtt ggattgagtt cagggacaat
     3541 tgatgaattt acaatgtcta taggaaagca gagcttgtta gcagtggttg attggttaac
     3601 tacacgaaaa ataaataact tcggtatgtt ggcttccagc ttatctgcgc ggatagctta
     3661 tgcaagccta tctgaaatca atgcttcgtt tttaatcacc gcagtcggtg ttgttaactt
     3721 aagatattct cttgaaagag ctttagggtt tgattatctc agtctaccca ttaatgaatt
     3781 gccggataat ctggattttg aaggccataa attgggtgct gaagtctttg cgagagattg
     3841 tcttgatttt ggttgggaag atttagcttc tacaattaat aacatgatgt atcttgatat
     3901 accgtttatt gcttttactg caaataacga taattgggtc aagcaagatg aagttatcac
     3961 attgttatca aatattcgta gtaatcgatg caagatatat tctttgttag gaagttcgca
     4021 tgacttgagt gaaaatttag tggtcctgcg caatttttat caatcggtta cgaaagccgc
     4081 tatcgcgatg gataatgatc atctggatat tgatgttgat attactgaac cgtcatttga
     4141 acatttaact attgcgacag tcaatgaacg ccgaatgaga attgagattg aaaatcaagc
     4201 aatttctctg tcttaaaatc tattgagata ttctatcact caaatagcaa tataaggact
     4261 ctctatgaaa tttggaaact ttttgcttac ataccaacct ccccaatttt ctcaaacaga
     4321 ggtaatgaaa cgtttggtta aattaggtcg catctctgag gagtgtggtt ttgataccgt
     4381 atggttactg gagcatcatt tcacggagtt tggtttgctt ggtaaccctt atgtcgctgc
     4441 tgcatattta cttggcgcga ctaaaaaatt gaatgtagga actgccgcta ttgttcttcc
     4501 cacagcccat ccagtacgcc aacttgaaga tgtgaattta ttggatcaaa tgtcaaaagg
     4561 acgatttcgg tttggtattt gccgagggct ttacaacaag gactttcgcg tattcggcac
     4621 agatatgaat aacagtcgcg ccttagcgga atgctggtac gggctgataa agaatggcat
     4681 gacagaggga tatatggaag ctgataatga acatatcaag ttccataagg taaaagtaaa
     4741 ccccgcggcg tatagcagag gtggcgcacc ggtttatgtg gtggctgaat cagcttcgac
     4801 gactgagtgg gctgctcaat ttggcctacc gatgatatta agttggatta taaatactaa
     4861 cgaaaagaaa gcacaacttg agctttataa tgaagtggct caagaatatg ggcacgatat
     4921 tcataatatc gaccattgct tatcatatat aacatctgta gatcatgact caattaaagc
     4981 gaaagagatt tgccggaaat ttctggggca ttggtatgat tcttatgtga atgctacgac
     5041 tatttttgat gattcagacc aaacaagagg ttatgatttc aataaagggc agtggcgtga
     5101 ctttgtatta aaaggacata aagatactaa tcgccgtatt gattacagtt acgaaatcaa
     5161 tcccgtggga acgccgcagg aatgtattga cataattcaa aaagacattg atgctacagg
     5221 aatatcaaat atttgttgtg gatttgaagc taatggaaca gtagacgaaa ttattgcttc
     5281 catgaagctc ttccagtctg atgtcatgcc atttcttaaa gaaaaacaac gttcgctatt
     5341 atattagcta aggagaaaga aatgaaattt ggattgttct tccttaactt catcaattca
     5401 acaactgttc aagaacaaag tatagttcgt atgcaggaaa taacggagta tgttgataag
     5461 ttgaattttg aacagatttt agtgtatgaa aatcattttt cagataatgg tgttgtcggc
     5521 gctcctctga ctgtttctgg ttttctgctc ggtttaacag agaaaattaa aattggttca
     5581 ttaaatcaca tcattacaac tcatcatcct gtcgccatag cggaggaagc gtgcttattg
     5641 gatcagttaa gtgaagggag atttatttta gggtttagtg attgcgaaaa aaaagatgaa
     5701 atgcattttt ttaatcgccc ggttgaatat caacagcaac tatttgaaga gtgttatgaa
     5761 atcattaacg atgctttaac aacaggctat tgtaatccag ataacgattt ttatagcttc
     5821 cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg gacctcggaa atatgtaaca
     5881 gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag gtattcctct catctttaag
     5941 tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa gatataaagc cgttgcggat
     6001 aaatatgacg ttgacctatc agagatagac catcagttaa tgatattagt taactataac
     6061 gaagatagta ataaagctaa acaagagacg cgtgcattta ttagtgatta tgttcttgaa
     6121 atgcacccta atgaaaattt cgaaaataaa cttgaagaaa taattgcaga aaacgctgtc
     6181 ggaaattata cggagtgtat aactgcggct aagttggcaa ttgaaaagtg tggtgcgaaa
     6241 agtgtattgc tgtcctttga accaatgaat gatttgatga gccaaaaaaa tgtaatcaat
     6301 attgttgatg ataatattaa gaagtaccac atggaatata cctaatagat ttcgagttgc
     6361 agcgaggcgg caagtgaacg aatccccagg agcatagata actatgtgac tggggtgagt
     6421 gaaagcagcc aacaaagcag cagcttgaaa gatgaagggt ataaaagagt atgacagcag
     6481 tgctgccata ctttctaata ttatcttgag gagtaaaaca ggtatgactt catatgttga
     6541 taaacaagaa attacagcaa gctcagaaat tgatgatttg attttttcga gcgatccatt
     6601 agtgtggtct tacgacgagc aggaaaaaat cagaaagaaa cttgtgcttg atgcatttcg
     6661 taatcattat aaacattgtc gagaatatcg tcactactgt caggcacaca aagtagatga
     6721 caatattacg gaaattgatg acatacctgt attcccaaca tcggttttta agtttactcg
     6781 cttattaact tctcaggaaa acgagattga aagttggttt accagtagcg gcacgaatgg
     6841 tttaaaaagt caggtggcgc gtgacagatt aagtattgag agactcttag gctctgtgag
     6901 ttatggcatg aaatatgttg gtagttggtt tgatcatcaa atagaattag tcaatttggg
     6961 accagataga tttaatgctc ataatatttg gtttaaatat gttatgagtt tggtggaatt
     7021 gttatatcct acgacattta ccgtaacaga agaacgaata gattttgtta aaacattgaa
     7081 tagtcttgaa cgaataaaaa atcaagggaa agatctttgt cttattggtt cgccatactt
     7141 tatttattta ctctgccatt atatgaaaga taaaaaaatc tcattttctg gagataaaag
     7201 cctttatatc ataaccggag gcggctggaa aagttacgaa aaagaatctc tgaaacgtga
     7261 tgatttcaat catcttttat ttgatacttt caatctcagt gatattagtc agatccgaga
     7321 tatatttaat caagttgaac tcaacacttg tttctttgag gatgaaatgc agcgtaaaca
     7381 tgttccgccg tgggtatatg cgcgagcgct tgatcctgaa acgttgaaac ctgtacctga
     7441 tggaacgccg gggttgatga gttatatgga tgcgtcagca accagttatc cagcatttat
     7501 tgttaccgat gatgtcggga taattagcag agaatatggt aagtatcccg gcgtgctcgt
     7561 tgaaatttta cgtcgcgtca atacgaggac gcagaaaggg tgtgctttga gcttaaccga
     7621 agcgtttgat agttgatatc ctttgcctaa ttgtaagtgg aatgcttgcg acccagcttt
     7681 cttgtacaaa gtggtgatgg ggatcctcta gagaatagga acttcggaat aggaacttca
     7741 agatcccctg attccctttg tcaacagcaa tggataattc gattaacaaa tgcatggcgc
     7801 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     7861 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     7921 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     7981 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     8041 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
     8101 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     8161 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     8221 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     8281 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     8341 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     8401 ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa
     8461 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     8521 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     8581 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     8641 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     8701 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     8761 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     8821 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     8881 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga
     8941 ggatccaaca tttccaatca cttaattaat tattccattg agtaagtttt taagcacatc
     9001 agcttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaataggt
     9061 acttcaagat ccccaattcg atcgtccggg ccgcaagctc ctagcggcgg atttgtccta
     9121 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
     9181 gcctttcgtt ttatttgatg cctcaagcta gagagtcatt accccaggcg tttaagggca
     9241 ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt ctagcttgga
     9301 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     9361 tctgaggtca ttactggatc tatcaacagg agtccaagct cagctaatta agctagctta
     9421 tcgataccgt cgacctcgaa ccccacgccc ctctttaata cgacgggcaa tttgcacttc
     9481 agaaaatgaa gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa
     9541 ctggactgat ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta
     9601 gatctatttt gttcagttta agactttatt gtccgcccac acccgcttac gcagggcatc
     9661 catttattac tcaaccgtaa ccgattttgc caggttacgc ggctggtcct ctagctggcg
     9721 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9781 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     9841 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     9901 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     9961 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
    10021 ga
//
