LOCUS       40924_43733        3633 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTn7xT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7-lux: A versatile tool for the identification and capture of 
            promoters in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2013) Microbiology, Immunology and Pathology, 
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO 
            80523, USA
REFERENCE   3  (bases 1 to 3633)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3633)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2013) Microbiology, Immunology and Pathology, Colorado State
            University, 2025 Campus Delivery, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..105
                     /label=FRT site
                     /note="FRT site"
     protein_bind    complement(58..105)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        157..185
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             512..745
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     protein_bind    complement(810..857)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(903..989)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(1092..1186)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1214..1230)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1235..1433
                     /note="Tn7R; Tn7 right end"
     oriT            2179..2287
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2310..2694
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  2910..3075
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    3106..3130
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3161..3626
                     /label=Pseudomonas aeruginosa PA4974 gene
                     /note="Pseudomonas aeruginosa PA4974 gene"
                     /regulatory_class="promoter"
ORIGIN
        1 gcccggggga tccactagtg agctcatgca tgatcgaatt agcttcaaaa gcgctctgaa
       61 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      121 cctttgtcaa cagcaatgga taattcacga acccagttga cataagcctg ttcggttcgt
      181 aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
      241 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag
      301 tctagcctcg ggcatccaag ctagctaagc gcgttacgcc gtgggtcgat gtttgatgtt
      361 atggaacagc aacgatgtta cgcagcaggg tagtcgccct aaaacaaagt taggcagccg
      421 ttgtgctggt gctttctagt agttgttgtg gggtaggcag tcagagctcg atttgcttgt
      481 cgccataata gattcacaag aaggattcga catgggtcaa agtagcgatg aagccaacgc
      541 tcccgttgca gggcagtttg cgcttcccct gagtgccacc tttggcttag gggatcgcgt
      601 acgcaagaaa tctggtgccg cttggcaggg tcaagtcgtc ggttggtatt gcacaaaact
      661 cactcctgaa ggctatgcgg tcgagtccga atcccaccca ggctcagtgc aaatttatcc
      721 tgtggctgca cttgaacgtg tggcctaaga attatctagg attattccat tgagtaagtt
      781 tttaagcaca tcagcttcaa aagcgctctg aagttcctat actttctaga gaataggaac
      841 ttcggaatag gtacttcaag atccccaatt cgatcgtccg ggccgcaagc tcctagcggc
      901 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      961 tctttcgact gagcctttcg ttttatttga tgcctcaagc tagagagtca ttaccccagg
     1021 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
     1081 gtctagcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1141 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag ctcagctaat
     1201 taagctagct tatcgatacc gtcgacctcg aaccccacgc ccctctttaa tacgacgggc
     1261 aatttgcact tcagaaaatg aagagtttgc tttagccata acaaaagtcc agtatgcttt
     1321 ttcacagcat aactggactg atttcagttt acaactattc tgtctagttt aagactttat
     1381 tgtcatagtt tagatctatt ttgttcagtt taagacttta ttgtccgccc acacccgctt
     1441 acgcagggca tccatttatt actcaaccgt aaccgatttt gccaggttac gcggctggtc
     1501 ctctagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     1561 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     1621 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     1681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1921 tatttgttta tttttctaaa tacattcaaa tatgtatccg gggcttccgc ttcctcgctc
     1981 actgacccgc tgcgctcggt cgttcggctg cggcgagcgg tatcagggga ttccttaagg
     2041 tatactttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     2101 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     2161 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     2221 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     2281 ctggccgata agctctgata acgcaggaaa gaacatgggc agttcaacct gttgatagta
     2341 cgtactaagc tctcatgttt cacgtactaa gctctcaagt ttaacgtact aagctctcat
     2401 gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa cgtactaagc
     2461 tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat aaatcagcaa
     2521 cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat aaggctttta
     2581 aagcttttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca ctgagaagcc
     2641 cttagagcct ctcaaagcaa ttttgagtga cacaggaaca cttaacggct gacaaccggc
     2701 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2761 gcctcccctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2821 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcgcc
     2881 caaacataac aggaagaaaa atgccccgct gtgggcggac aaaatagttg ggaactggga
     2941 ggggtggaaa tggagttttt aaggattatt tagggaagag tgacaaaata gatgggaact
     3001 gggtgtagcg tcgtaagcta atacgaaaat taaaaatgac aaaatagttt ggaactagat
     3061 ttcacttatc tggttggcct gcaaggcctt cgcgaggtac ccatcacaag tttgtacaaa
     3121 aaagcaggct ttaattaatc gaggcgcgcc atcgcactat gtgatcgatg gctctcctgg
     3181 gtgattgtcg ggagaacctg gcacgcgggg ccaccgggac tggtggcgag aaggacgcgc
     3241 cggcaaggca ggcatggcgc ggacatcttg ttccctacgc gggttgctgc ggtccgcgcg
     3301 aggcggaacc ggacgctagc ccgatcaggt tcaacgggat ccggaactat ccgatctcag
     3361 cccctcgtat agggcacccc gacaagaacc ccggcagtct aatcaacacc ggcgggatac
     3421 gccaacggga aaacgccccg cccgaccggc agtcatggtc cagtccggcg ccctctcgcc
     3481 agggaagacg cagatccccg cacgttcgcc ggcacatcga tccggactgg ctcttcgatc
     3541 agttgccgcg caggtgcctt gaccgccacc ggtagcgacc ttagccttgc cccacctcgc
     3601 atcttttcca ggacgaacga cacgcggtgc tgc
//
