LOCUS       40924_43708        3850 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTn7xG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Bruckbauer ST.
  TITLE     Tn5/7-lux: A versatile tool for the identification and capture of 
            promoters in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Bruckbauer ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2013) Microbiology, Immunology and Pathology, 
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO 
            80523, USA
REFERENCE   3  (bases 1 to 3850)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2013) Microbiology, Immunology and Pathology, Colorado State
            University, 2025 Campus Delivery, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..105
                     /label=FRT site
                     /note="FRT site"
     protein_bind    complement(58..105)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        146..174
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             363..893
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     protein_bind    complement(1026..1073)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(1119..1205)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(1308..1402)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1430..1446)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1451..1650
                     /note="Tn7R; Tn7 right end"
     oriT            2395..2503
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2526..2910
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  3126..3291
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    3322..3346
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3377..3842
                     /label=Pseudomonas aeruginosa PA4974 gene
                     /note="Pseudomonas aeruginosa PA4974 gene"
                     /regulatory_class="promoter"
ORIGIN
        1 gcccggggga tccactagtg agctcatgca tgatcgaatt agcttcaaaa gcgctctgaa
       61 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      121 cctttgtcaa cagcaatgga tcgaattgac ataagcctgt tcggttcgta aactgtaatg
      181 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      241 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt ctatgcctcg
      301 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
      361 cgatgttacg cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag
      421 gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca
      481 tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac
      541 atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg
      601 ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg
      661 agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc
      721 agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt
      781 atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt
      841 tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat
      901 tcgttcaagc cgagatcggc ttcccggccg cggagttgtt cggtaaattg tcacaacgcc
      961 gcggccaatt cgatctagaa ttccattgag taagttttta agcacatcag cttcaaaagc
     1021 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggtac ttcaagatcc
     1081 ccaattcgat cgtccgggcc gcaagctcct agcggcggat ttgtcctact caggagagcg
     1141 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     1201 atttgatgcc tcaagctaga gagtcattac cccaggcgtt taagggcacc aataactgcc
     1261 ttaaaaaaat tacgccccgc cctgccactc atcgcagtct agcttggatt ctcaccaata
     1321 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     1381 actggatcta tcaacaggag tccaagctca gctaattaag ctagcttatc gataccgtcg
     1441 acctcgaacc ccacgcccct ctttaatacg acgggcaatt tgcacttcag aaaatgaaga
     1501 gtttgcttta gccataacaa aagtccagta tgctttttca cagcataact ggactgattt
     1561 cagtttacaa ctattctgtc tagtttaaga ctttattgtc atagtttaga tctattttgt
     1621 tcagtttaag actttattgt ccgcccacac ccgcttacgc agggcatcca tttattactc
     1681 aaccgtaacc gattttgcca ggttacgcgg ctggtcctct agctggcgta atagcgaaga
     1741 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     1801 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     1861 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     1921 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     1981 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2041 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2161 ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg ctcggtcgtt
     2221 cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg cataaccctg
     2281 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     2341 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     2401 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     2461 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct ctgataacgc
     2521 aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg
     2581 tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg
     2641 ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc
     2701 tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa
     2761 gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt
     2821 ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt
     2881 gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta gcagagcgag
     2941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg cagccgaacg
     3001 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3061 ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga agaaaaatgc
     3121 cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga gtttttaagg
     3181 attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt aagctaatac
     3241 gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt tggcctgcaa
     3301 ggccttcgcg aggtacccat cacaagtttg tacaaaaaag caggctttaa ttaatcgagg
     3361 cgcgccatcg cactatgtga tcgatggctc tcctgggtga ttgtcgggag aacctggcac
     3421 gcggggccac cgggactggt ggcgagaagg acgcgccggc aaggcaggca tggcgcggac
     3481 atcttgttcc ctacgcgggt tgctgcggtc cgcgcgaggc ggaaccggac gctagcccga
     3541 tcaggttcaa cgggatccgg aactatccga tctcagcccc tcgtataggg caccccgaca
     3601 agaaccccgg cagtctaatc aacaccggcg ggatacgcca acgggaaaac gccccgcccg
     3661 accggcagtc atggtccagt ccggcgccct ctcgccaggg aagacgcaga tccccgcacg
     3721 ttcgccggca catcgatccg gactggctct tcgatcagtt gccgcgcagg tgccttgacc
     3781 gccaccggta gcgaccttag ccttgcccca cctcgcatct tttccaggac gaacgacacg
     3841 cggtgctgca
//
