LOCUS       40924_43703        9661 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter capture vector pTn7tluxK3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux : A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 9661)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    1292..1316
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1478..2917
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             2932..3852
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             3904..4983
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5001..5981
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             6163..7272
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     protein_bind    complement(7310..7334)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7764..8555
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(8657..8704)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(8750..8836)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(8939..9033)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9061..9077)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9082..9280
                     /label=Tn7R
                     /note="Tn7R"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg
      181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg
      241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
      301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
      361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
      421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct
      481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc
      541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa
      601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
      661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
      721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
      781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
      841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg
      961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga
     1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     1261 tggcctgcaa ggccttcgcg aggtacccat cacaagtttg tacaaaaaag caggctttaa
     1321 ttaatcgagg cgcgccatcg cactatgtgg cgattacaag ccggaatcag gctatcatgc
     1381 actcaagttg gaaacaagat ggccggtccc gtcttatatc ggtttttgac gatcctgaat
     1441 tcacccacgc ggtgcattaa atggatggca aatatgaatg actaaaaaaa tttcattcat
     1501 tattaacggc caggttgaaa tctttcccga aagtgatgat ttagtgcaat ccattaattt
     1561 tggtgataat agtgtttacc tgccaatatt gaatgactct catgtaaaaa acattattga
     1621 ttgtaatgga aataacgaat tacggttgca taacattgtc aattttctct atacggtagg
     1681 gcaaagatgg aaaaatgaag aatactcaag acgcaggaca tacattcgtg acttaaaaaa
     1741 atatatggga tattcagaag aaatggctaa gctagaggcc aattggatat ctatgatttt
     1801 atgttctaaa ggcggccttt atgatgttgt agaaaatgaa cttggttctc gccatatcat
     1861 ggatgaatgg ctacctcagg atgaaagtta tgttcgggct tttccgaaag gtaaatctgt
     1921 acatctgttg gcaggtaatg ttccattatc tgggatcatg tctatattac gcgcaatttt
     1981 aactaagaat cagtgtatta taaaaacatc gtcaaccgat ccttttaccg ctaatgcatt
     2041 agcgttaagt tttattgatg tagaccctaa tcatccgata acgcgctctt tatctgttat
     2101 atattggccc caccaaggtg atacatcact cgcaaaagaa attatgcgac atgcggatgt
     2161 tattgtcgct tggggagggc cagatgcgat taattgggcg gtagagcacg cgccatctta
     2221 tgctgatgtg attaaatttg gttctaaaaa gagtctttgc attatcgata atcctgttga
     2281 tttgacgtcc gcagcgacag gtgcggctca tgatgtttgt ttttacgatc agcgagcttg
     2341 tttttctgcc caaaacatat attacatggg aaatcattat gaggaattta agttagcgtt
     2401 gatagaaaaa cttaatctat atgcgcatat attaccgaat gccaaaaaag attttgatga
     2461 aaaggcggcc tattctttag ttcaaaaaga aagcctgttt gctggattaa aagtagaggt
     2521 ggatattcat caacgttgga tgattattga gtcaaatgca ggtgtggaat ttaatcaacc
     2581 acttggcaga tgtgtgtacc ttcatcacgt cgataatatt gagcaaatat tgccttatgt
     2641 tcaaaaaaat aagacgcaaa ccatatctat ttttccttgg gagtcatcat ttaaatatcg
     2701 agatgcgtta gcattaaaag gtgcggaaag gattgtagaa gcaggaatga ataacatatt
     2761 tcgagttggt ggatctcatg acggaatgag accgttgcaa cgattagtga catatatttc
     2821 tcatgaaagg ccatctaact atacggctaa ggatgttgcg gttgaaatag aacagactcg
     2881 attcctggaa gaagataagt tccttgtatt tgtcccataa taggtaaaag tatggaaaat
     2941 gaatcaaaat ataaaaccat cgaccacgtt atttgtgttg aaggaaataa aaaaattcat
     3001 gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa agaatgccat tattattgcg
     3061 tctggttttg cccgcaggat ggatcatttt gctggtctgg cggaatattt atcgcggaat
     3121 ggatttcatg tgatccgcta tgattcgctt caccacgttg gattgagttc agggacaatt
     3181 gatgaattta caatgtctat aggaaagcag agcttgttag cagtggttga ttggttaact
     3241 acacgaaaaa taaataactt cggtatgttg gcttccagct tatctgcgcg gatagcttat
     3301 gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta
     3361 agatattctc ttgaaagagc tttagggttt gattatctca gtctacccat taatgaattg
     3421 ccggataatc tggattttga aggccataaa ttgggtgctg aagtctttgc gagagattgt
     3481 cttgattttg gttgggaaga tttagcttct acaattaata acatgatgta tcttgatata
     3541 ccgtttattg cttttactgc aaataacgat aattgggtca agcaagatga agttatcaca
     3601 ttgttatcaa atattcgtag taatcgatgc aagatatatt ctttgttagg aagttcgcat
     3661 gacttgagtg aaaatttagt ggtcctgcgc aatttttatc aatcggttac gaaagccgct
     3721 atcgcgatgg ataatgatca tctggatatt gatgttgata ttactgaacc gtcatttgaa
     3781 catttaacta ttgcgacagt caatgaacgc cgaatgagaa ttgagattga aaatcaagca
     3841 atttctctgt cttaaaatct attgagatat tctatcactc aaatagcaat ataaggactc
     3901 tctatgaaat ttggaaactt tttgcttaca taccaacctc cccaattttc tcaaacagag
     3961 gtaatgaaac gtttggttaa attaggtcgc atctctgagg agtgtggttt tgataccgta
     4021 tggttactgg agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct
     4081 gcatatttac ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat tgttcttccc
     4141 acagcccatc cagtacgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga
     4201 cgatttcggt ttggtatttg ccgagggctt tacaacaagg actttcgcgt attcggcaca
     4261 gatatgaata acagtcgcgc cttagcggaa tgctggtacg ggctgataaa gaatggcatg
     4321 acagagggat atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac
     4381 cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg
     4441 actgagtggg ctgctcaatt tggcctaccg atgatattaa gttggattat aaatactaac
     4501 gaaaagaaag cacaacttga gctttataat gaagtggctc aagaatatgg gcacgatatt
     4561 cataatatcg accattgctt atcatatata acatctgtag atcatgactc aattaaagcg
     4621 aaagagattt gccggaaatt tctggggcat tggtatgatt cttatgtgaa tgctacgact
     4681 atttttgatg attcagacca aacaagaggt tatgatttca ataaagggca gtggcgtgac
     4741 tttgtattaa aaggacataa agatactaat cgccgtattg attacagtta cgaaatcaat
     4801 cccgtgggaa cgccgcagga atgtattgac ataattcaaa aagacattga tgctacagga
     4861 atatcaaata tttgttgtgg atttgaagct aatggaacag tagacgaaat tattgcttcc
     4921 atgaagctct tccagtctga tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta
     4981 tattagctaa ggagaaagaa atgaaatttg gattgttctt ccttaacttc atcaattcaa
     5041 caactgttca agaacaaagt atagttcgta tgcaggaaat aacggagtat gttgataagt
     5101 tgaattttga acagatttta gtgtatgaaa atcatttttc agataatggt gttgtcggcg
     5161 ctcctctgac tgtttctggt tttctgctcg gtttaacaga gaaaattaaa attggttcat
     5221 taaatcacat cattacaact catcatcctg tcgccatagc ggaggaagcg tgcttattgg
     5281 atcagttaag tgaagggaga tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa
     5341 tgcatttttt taatcgcccg gttgaatatc aacagcaact atttgaagag tgttatgaaa
     5401 tcattaacga tgctttaaca acaggctatt gtaatccaga taacgatttt tatagcttcc
     5461 ctaaaatatc tgtaaatccc catgcttata cgccaggcgg acctcggaaa tatgtaacag
     5521 caaccagtca tcatattgtt gagtgggcgg ccaaaaaagg tattcctctc atctttaagt
     5581 gggatgattc taatgatgtt agatatgaat atgctgaaag atataaagcc gttgcggata
     5641 aatatgacgt tgacctatca gagatagacc atcagttaat gatattagtt aactataacg
     5701 aagatagtaa taaagctaaa caagagacgc gtgcatttat tagtgattat gttcttgaaa
     5761 tgcaccctaa tgaaaatttc gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg
     5821 gaaattatac ggagtgtata actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa
     5881 gtgtattgct gtcctttgaa ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata
     5941 ttgttgatga taatattaag aagtaccaca tggaatatac ctaatagatt tcgagttgca
     6001 gcgaggcggc aagtgaacga atccccagga gcatagataa ctatgtgact ggggtgagtg
     6061 aaagcagcca acaaagcagc agcttgaaag atgaagggta taaaagagta tgacagcagt
     6121 gctgccatac tttctaatat tatcttgagg agtaaaacag gtatgacttc atatgttgat
     6181 aaacaagaaa ttacagcaag ctcagaaatt gatgatttga ttttttcgag cgatccatta
     6241 gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt
     6301 aatcattata aacattgtcg agaatatcgt cactactgtc aggcacacaa agtagatgac
     6361 aatattacgg aaattgatga catacctgta ttcccaacat cggtttttaa gtttactcgc
     6421 ttattaactt ctcaggaaaa cgagattgaa agttggttta ccagtagcgg cacgaatggt
     6481 ttaaaaagtc aggtggcgcg tgacagatta agtattgaga gactcttagg ctctgtgagt
     6541 tatggcatga aatatgttgg tagttggttt gatcatcaaa tagaattagt caatttggga
     6601 ccagatagat ttaatgctca taatatttgg tttaaatatg ttatgagttt ggtggaattg
     6661 ttatatccta cgacatttac cgtaacagaa gaacgaatag attttgttaa aacattgaat
     6721 agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc ttattggttc gccatacttt
     6781 atttatttac tctgccatta tatgaaagat aaaaaaatct cattttctgg agataaaagc
     6841 ctttatatca taaccggagg cggctggaaa agttacgaaa aagaatctct gaaacgtgat
     6901 gatttcaatc atcttttatt tgatactttc aatctcagtg atattagtca gatccgagat
     6961 atatttaatc aagttgaact caacacttgt ttctttgagg atgaaatgca gcgtaaacat
     7021 gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat
     7081 ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa ccagttatcc agcatttatt
     7141 gttaccgatg atgtcgggat aattagcaga gaatatggta agtatcccgg cgtgctcgtt
     7201 gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt gtgctttgag cttaaccgaa
     7261 gcgtttgata gttgatatcc tttgcctaat tgtaagtgga atgcttgcga cccagctttc
     7321 ttgtacaaag tggtgatggg gatcctctag agaataggaa cttcggaata ggaacttcaa
     7381 gatcccctga ttccctttgt caacagcaat ggataattcg attaacaaat gcatggcgca
     7441 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     7501 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     7561 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     7621 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     7681 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag
     7741 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     7801 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7861 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7921 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     7981 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     8041 tattgggcga agtgccgggg caggatctcc tgtcatccca ccttgctcct gccgagaaag
     8101 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     8161 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     8221 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     8281 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     8341 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     8401 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     8461 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     8521 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga agagtatgag
     8581 gatccaacat ttccaatcac ttaattaatt attccattga gtaagttttt aagcacatca
     8641 gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggta
     8701 cttcaagatc cccaattcga tcgtccgggc cgcaagctcc tagcggcgga tttgtcctac
     8761 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     8821 cctttcgttt tatttgatgc ctcaagctag agagtcatta ccccaggcgt ttaagggcac
     8881 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagtc tagcttggat
     8941 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     9001 ctgaggtcat tactggatct atcaacagga gtccaagctc agctaattaa gctagcttat
     9061 cgataccgtc gacctcgaac cccacgcccc tctttaatac gacgggcaat ttgcacttca
     9121 gaaaatgaag agtttgcttt agccataaca aaagtccagt atgctttttc acagcataac
     9181 tggactgatt tcagtttaca actattctgt ctagtttaag actttattgt catagtttag
     9241 atctattttg ttcagtttaa gactttattg tccgcccaca cccgcttacg cagggcatcc
     9301 atttattact caaccgtaac cgattttgcc aggttacgcg gctggtcctc tagctggcgt
     9361 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9421 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     9481 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     9541 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     9601 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     9661 a
//
