LOCUS       40924_43693        9823 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter capture vector pTn7oluxK4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux : A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 9823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    1292..1316
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1350..1548
                     /label=PompA region
                     /note="PompA region"
                     /regulatory_class="promoter"
     CDS             1581..3020
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             3035..3955
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             4007..5086
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             5104..6084
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             6266..7375
                     /label=LuxE
                     /note="LuxE"
     protein_bind    complement(7413..7437)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     7493..7540
                     /label=FRT
                     /note="FRT"
     protein_bind    complement(7493..7540)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7926..8717
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(8819..8866)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(8912..8998)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9101..9195)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9223..9239)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9244..9442
                     /label=Tn7R
                     /note="Tn7R"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg
      181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg
      241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
      301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
      361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
      421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct
      481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc
      541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa
      601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
      661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
      721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
      781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
      841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg
      961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga
     1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     1261 tggcctgcaa ggccttcgcg aggtacccat cacaagtttg tacaaaaaag caggctttaa
     1321 ttaatcgagg cgcgccatcg cactatgtga gaccgatgtt agggtgggga ggattttttt
     1381 tcgtgggaag ggaacgatat ggcaaaatgc catcgcactg aaagttagac gctgctcgaa
     1441 gtctacacag agcggtgccg cgttttttgt gccgcaccaa tgttatactt cgagcaatgt
     1501 atgacttgtc atcgtcaaca ggctcgcagt aaacctgcgg taatctcaca cgcggtgcat
     1561 taaatggatg gcaaatatga atgactaaaa aaatttcatt cattattaac ggccaggttg
     1621 aaatctttcc cgaaagtgat gatttagtgc aatccattaa ttttggtgat aatagtgttt
     1681 acctgccaat attgaatgac tctcatgtaa aaaacattat tgattgtaat ggaaataacg
     1741 aattacggtt gcataacatt gtcaattttc tctatacggt agggcaaaga tggaaaaatg
     1801 aagaatactc aagacgcagg acatacattc gtgacttaaa aaaatatatg ggatattcag
     1861 aagaaatggc taagctagag gccaattgga tatctatgat tttatgttct aaaggcggcc
     1921 tttatgatgt tgtagaaaat gaacttggtt ctcgccatat catggatgaa tggctacctc
     1981 aggatgaaag ttatgttcgg gcttttccga aaggtaaatc tgtacatctg ttggcaggta
     2041 atgttccatt atctgggatc atgtctatat tacgcgcaat tttaactaag aatcagtgta
     2101 ttataaaaac atcgtcaacc gatcctttta ccgctaatgc attagcgtta agttttattg
     2161 atgtagaccc taatcatccg ataacgcgct ctttatctgt tatatattgg ccccaccaag
     2221 gtgatacatc actcgcaaaa gaaattatgc gacatgcgga tgttattgtc gcttggggag
     2281 ggccagatgc gattaattgg gcggtagagc acgcgccatc ttatgctgat gtgattaaat
     2341 ttggttctaa aaagagtctt tgcattatcg ataatcctgt tgatttgacg tccgcagcga
     2401 caggtgcggc tcatgatgtt tgtttttacg atcagcgagc ttgtttttct gcccaaaaca
     2461 tatattacat gggaaatcat tatgaggaat ttaagttagc gttgatagaa aaacttaatc
     2521 tatatgcgca tatattaccg aatgccaaaa aagattttga tgaaaaggcg gcctattctt
     2581 tagttcaaaa agaaagcctg tttgctggat taaaagtaga ggtggatatt catcaacgtt
     2641 ggatgattat tgagtcaaat gcaggtgtgg aatttaatca accacttggc agatgtgtgt
     2701 accttcatca cgtcgataat attgagcaaa tattgcctta tgttcaaaaa aataagacgc
     2761 aaaccatatc tatttttcct tgggagtcat catttaaata tcgagatgcg ttagcattaa
     2821 aaggtgcgga aaggattgta gaagcaggaa tgaataacat atttcgagtt ggtggatctc
     2881 atgacggaat gagaccgttg caacgattag tgacatatat ttctcatgaa aggccatcta
     2941 actatacggc taaggatgtt gcggttgaaa tagaacagac tcgattcctg gaagaagata
     3001 agttccttgt atttgtccca taataggtaa aagtatggaa aatgaatcaa aatataaaac
     3061 catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt catgtttggg aaacgctgcc
     3121 agaagaaaac agcccaaaga gaaagaatgc cattattatt gcgtctggtt ttgcccgcag
     3181 gatggatcat tttgctggtc tggcggaata tttatcgcgg aatggatttc atgtgatccg
     3241 ctatgattcg cttcaccacg ttggattgag ttcagggaca attgatgaat ttacaatgtc
     3301 tataggaaag cagagcttgt tagcagtggt tgattggtta actacacgaa aaataaataa
     3361 cttcggtatg ttggcttcca gcttatctgc gcggatagct tatgcaagcc tatctgaaat
     3421 caatgcttcg tttttaatca ccgcagtcgg tgttgttaac ttaagatatt ctcttgaaag
     3481 agctttaggg tttgattatc tcagtctacc cattaatgaa ttgccggata atctggattt
     3541 tgaaggccat aaattgggtg ctgaagtctt tgcgagagat tgtcttgatt ttggttggga
     3601 agatttagct tctacaatta ataacatgat gtatcttgat ataccgttta ttgcttttac
     3661 tgcaaataac gataattggg tcaagcaaga tgaagttatc acattgttat caaatattcg
     3721 tagtaatcga tgcaagatat attctttgtt aggaagttcg catgacttga gtgaaaattt
     3781 agtggtcctg cgcaattttt atcaatcggt tacgaaagcc gctatcgcga tggataatga
     3841 tcatctggat attgatgttg atattactga accgtcattt gaacatttaa ctattgcgac
     3901 agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa gcaatttctc tgtcttaaaa
     3961 tctattgaga tattctatca ctcaaatagc aatataagga ctctctatga aatttggaaa
     4021 ctttttgctt acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt
     4081 taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca
     4141 tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc
     4201 gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg
     4261 ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat
     4321 ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg
     4381 cgccttagcg gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga
     4441 agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag
     4501 aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca
     4561 atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact
     4621 tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg
     4681 cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa
     4741 atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga
     4801 ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca
     4861 taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca
     4921 ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg
     4981 tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc
     5041 tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttatattagc taaggagaaa
     5101 gaaatgaaat ttggattgtt cttccttaac ttcatcaatt caacaactgt tcaagaacaa
     5161 agtatagttc gtatgcagga aataacggag tatgttgata agttgaattt tgaacagatt
     5221 ttagtgtatg aaaatcattt ttcagataat ggtgttgtcg gcgctcctct gactgtttct
     5281 ggttttctgc tcggtttaac agagaaaatt aaaattggtt cattaaatca catcattaca
     5341 actcatcatc ctgtcgccat agcggaggaa gcgtgcttat tggatcagtt aagtgaaggg
     5401 agatttattt tagggtttag tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc
     5461 ccggttgaat atcaacagca actatttgaa gagtgttatg aaatcattaa cgatgcttta
     5521 acaacaggct attgtaatcc agataacgat ttttatagct tccctaaaat atctgtaaat
     5581 ccccatgctt atacgccagg cggacctcgg aaatatgtaa cagcaaccag tcatcatatt
     5641 gttgagtggg cggccaaaaa aggtattcct ctcatcttta agtgggatga ttctaatgat
     5701 gttagatatg aatatgctga aagatataaa gccgttgcgg ataaatatga cgttgaccta
     5761 tcagagatag accatcagtt aatgatatta gttaactata acgaagatag taataaagct
     5821 aaacaagaga cgcgtgcatt tattagtgat tatgttcttg aaatgcaccc taatgaaaat
     5881 ttcgaaaata aacttgaaga aataattgca gaaaacgctg tcggaaatta tacggagtgt
     5941 ataactgcgg ctaagttggc aattgaaaag tgtggtgcga aaagtgtatt gctgtccttt
     6001 gaaccaatga atgatttgat gagccaaaaa aatgtaatca atattgttga tgataatatt
     6061 aagaagtacc acatggaata tacctaatag atttcgagtt gcagcgaggc ggcaagtgaa
     6121 cgaatcccca ggagcataga taactatgtg actggggtga gtgaaagcag ccaacaaagc
     6181 agcagcttga aagatgaagg gtataaaaga gtatgacagc agtgctgcca tactttctaa
     6241 tattatcttg aggagtaaaa caggtatgac ttcatatgtt gataaacaag aaattacagc
     6301 aagctcagaa attgatgatt tgattttttc gagcgatcca ttagtgtggt cttacgacga
     6361 gcaggaaaaa atcagaaaga aacttgtgct tgatgcattt cgtaatcatt ataaacattg
     6421 tcgagaatat cgtcactact gtcaggcaca caaagtagat gacaatatta cggaaattga
     6481 tgacatacct gtattcccaa catcggtttt taagtttact cgcttattaa cttctcagga
     6541 aaacgagatt gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa gtcaggtggc
     6601 gcgtgacaga ttaagtattg agagactctt aggctctgtg agttatggca tgaaatatgt
     6661 tggtagttgg tttgatcatc aaatagaatt agtcaatttg ggaccagata gatttaatgc
     6721 tcataatatt tggtttaaat atgttatgag tttggtggaa ttgttatatc ctacgacatt
     6781 taccgtaaca gaagaacgaa tagattttgt taaaacattg aatagtcttg aacgaataaa
     6841 aaatcaaggg aaagatcttt gtcttattgg ttcgccatac tttatttatt tactctgcca
     6901 ttatatgaaa gataaaaaaa tctcattttc tggagataaa agcctttata tcataaccgg
     6961 aggcggctgg aaaagttacg aaaaagaatc tctgaaacgt gatgatttca atcatctttt
     7021 atttgatact ttcaatctca gtgatattag tcagatccga gatatattta atcaagttga
     7081 actcaacact tgtttctttg aggatgaaat gcagcgtaaa catgttccgc cgtgggtata
     7141 tgcgcgagcg cttgatcctg aaacgttgaa acctgtacct gatggaacgc cggggttgat
     7201 gagttatatg gatgcgtcag caaccagtta tccagcattt attgttaccg atgatgtcgg
     7261 gataattagc agagaatatg gtaagtatcc cggcgtgctc gttgaaattt tacgtcgcgt
     7321 caatacgagg acgcagaaag ggtgtgcttt gagcttaacc gaagcgtttg atagttgata
     7381 tcctttgcct aattgtaagt ggaatgcttg cgacccagct ttcttgtaca aagtggtgat
     7441 ggggatccac tagtgagctc atgcatgatc gaattagctt caaaagcgct ctgaagttcc
     7501 tatactttct agagaatagg aacttcggaa taggaacttc aagatcccct gattcccttt
     7561 gtcaacagca atggataatt cgattaacaa atgcatggcg caagggctgc taaaggaagc
     7621 ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     7681 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     7741 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     7801 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
     7861 cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt
     7921 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     7981 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     8041 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     8101 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     8161 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     8221 ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg
     8281 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     8341 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     8401 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     8461 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     8521 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     8581 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     8641 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     8701 ttcttgacga gttcttctga attgaaaaag gaagagtatg aggatccaac atttccaatc
     8761 acttaattaa ttattccatt gagtaagttt ttaagcacat cagcttcaaa agcgctctga
     8821 agttcctata ctttctagag aataggaact tcggaatagg tacttcaaga tccccaattc
     8881 gatcgtccgg gccgcaagct cctagcggcg gatttgtcct actcaggaga gcgttcaccg
     8941 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     9001 gcctcaagct agagagtcat taccccaggc gtttaagggc accaataact gccttaaaaa
     9061 aattacgccc cgccctgcca ctcatcgcag tctagcttgg attctcacca ataaaaaacg
     9121 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     9181 ctatcaacag gagtccaagc tcagctaatt aagctagctt atcgataccg tcgacctcga
     9241 accccacgcc cctctttaat acgacgggca atttgcactt cagaaaatga agagtttgct
     9301 ttagccataa caaaagtcca gtatgctttt tcacagcata actggactga tttcagttta
     9361 caactattct gtctagttta agactttatt gtcatagttt agatctattt tgttcagttt
     9421 aagactttat tgtccgccca cacccgctta cgcagggcat ccatttatta ctcaaccgta
     9481 accgattttg ccaggttacg cggctggtcc tctagctggc gtaatagcga agaggcccgc
     9541 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     9601 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     9661 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     9721 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     9781 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cga
//
