LOCUS       40924_43673        9864 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTn7APH1AluxK5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux: A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 9864)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_feature    1273..1595
                     /label=A. baumannii promoter region
                     /note="A. baumannii promoter region"
     CDS             1622..3061
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             3076..3996
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4048..5127
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5145..6125
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             6307..7416
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     protein_bind    complement(7454..7478)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     7534..7581
                     /label=FRT
                     /note="FRT"
     protein_bind    complement(7534..7581)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7967..8758
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(8860..8907)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(8953..9039)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9142..9236)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9264..9280)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9285..9483
                     /label=Tn7R
                     /note="Tn7R"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg
      181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg
      241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
      301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
      361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
      421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct
      481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc
      541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa
      601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
      661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
      721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
      781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
      841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg
      961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga
     1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     1261 tggcctgcaa ggccttcttg cgaatcttct tcaatctcta ggctcattcg tgtcgttttc
     1321 ctataaataa aacagggttc gaattggggc attatgcttt attttagaac tattgtaatt
     1381 ataaagatta cataaaacaa tttttatatt taaaacattg tttccagctt gaaacgtaac
     1441 aaattattta aaatttatta attaattgat tttaatattt atttttactt tgacaaaaat
     1501 taatcaagag gattatgctt agcttgtgtt ttgaaaatca aattatgttt tgtatataaa
     1561 acaaaatgag gacgcaaggt atgagttgtc acgcggtgca ttaaatggat ggcaaatatg
     1621 aatgactaaa aaaatttcat tcattattaa cggccaggtt gaaatctttc ccgaaagtga
     1681 tgatttagtg caatccatta attttggtga taatagtgtt tacctgccaa tattgaatga
     1741 ctctcatgta aaaaacatta ttgattgtaa tggaaataac gaattacggt tgcataacat
     1801 tgtcaatttt ctctatacgg tagggcaaag atggaaaaat gaagaatact caagacgcag
     1861 gacatacatt cgtgacttaa aaaaatatat gggatattca gaagaaatgg ctaagctaga
     1921 ggccaattgg atatctatga ttttatgttc taaaggcggc ctttatgatg ttgtagaaaa
     1981 tgaacttggt tctcgccata tcatggatga atggctacct caggatgaaa gttatgttcg
     2041 ggcttttccg aaaggtaaat ctgtacatct gttggcaggt aatgttccat tatctgggat
     2101 catgtctata ttacgcgcaa ttttaactaa gaatcagtgt attataaaaa catcgtcaac
     2161 cgatcctttt accgctaatg cattagcgtt aagttttatt gatgtagacc ctaatcatcc
     2221 gataacgcgc tctttatctg ttatatattg gccccaccaa ggtgatacat cactcgcaaa
     2281 agaaattatg cgacatgcgg atgttattgt cgcttgggga gggccagatg cgattaattg
     2341 ggcggtagag cacgcgccat cttatgctga tgtgattaaa tttggttcta aaaagagtct
     2401 ttgcattatc gataatcctg ttgatttgac gtccgcagcg acaggtgcgg ctcatgatgt
     2461 ttgtttttac gatcagcgag cttgtttttc tgcccaaaac atatattaca tgggaaatca
     2521 ttatgaggaa tttaagttag cgttgataga aaaacttaat ctatatgcgc atatattacc
     2581 gaatgccaaa aaagattttg atgaaaaggc ggcctattct ttagttcaaa aagaaagcct
     2641 gtttgctgga ttaaaagtag aggtggatat tcatcaacgt tggatgatta ttgagtcaaa
     2701 tgcaggtgtg gaatttaatc aaccacttgg cagatgtgtg taccttcatc acgtcgataa
     2761 tattgagcaa atattgcctt atgttcaaaa aaataagacg caaaccatat ctatttttcc
     2821 ttgggagtca tcatttaaat atcgagatgc gttagcatta aaaggtgcgg aaaggattgt
     2881 agaagcagga atgaataaca tatttcgagt tggtggatct catgacggaa tgagaccgtt
     2941 gcaacgatta gtgacatata tttctcatga aaggccatct aactatacgg ctaaggatgt
     3001 tgcggttgaa atagaacaga ctcgattcct ggaagaagat aagttccttg tatttgtccc
     3061 ataataggta aaagtatgga aaatgaatca aaatataaaa ccatcgacca cgttatttgt
     3121 gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa cagcccaaag
     3181 agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca ttttgctggt
     3241 ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc gcttcaccac
     3301 gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa gcagagcttg
     3361 ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat gttggcttcc
     3421 agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc gtttttaatc
     3481 accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg gtttgattat
     3541 ctcagtctac ccattaatga attgccggat aatctggatt ttgaaggcca taaattgggt
     3601 gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc ttctacaatt
     3661 aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa cgataattgg
     3721 gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg atgcaagata
     3781 tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct gcgcaatttt
     3841 tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga tattgatgtt
     3901 gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga acgccgaatg
     3961 agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag atattctatc
     4021 actcaaatag caatataagg actctctatg aaatttggaa actttttgct tacataccaa
     4081 cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg tcgcatctct
     4141 gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga gtttggtttg
     4201 cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa attgaatgta
     4261 ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga agatgtgaat
     4321 ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg gctttacaac
     4381 aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc ggaatgctgg
     4441 tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa tgaacatatc
     4501 aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc accggtttat
     4561 gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct accgatgata
     4621 ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta taatgaagtg
     4681 gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata tataacatct
     4741 gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg gcattggtat
     4801 gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag aggttatgat
     4861 ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac taatcgccgt
     4921 attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat tgacataatt
     4981 caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga agctaatgga
     5041 acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat gccatttctt
     5101 aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa tttggattgt
     5161 tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt cgtatgcagg
     5221 aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat gaaaatcatt
     5281 tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg ctcggtttaa
     5341 cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat cctgtcgcca
     5401 tagcggagga agcgtgctta ttggatcagt taagtgaagg gagatttatt ttagggttta
     5461 gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa tatcaacagc
     5521 aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc tattgtaatc
     5581 cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct tatacgccag
     5641 gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg gcggccaaaa
     5701 aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat gaatatgctg
     5761 aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata gaccatcagt
     5821 taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag acgcgtgcat
     5881 ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat aaacttgaag
     5941 aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg gctaagttgg
     6001 caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg aatgatttga
     6061 tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac cacatggaat
     6121 atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc aggagcatag
     6181 ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg aaagatgaag
     6241 ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt gaggagtaaa
     6301 acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga aattgatgat
     6361 ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa aatcagaaag
     6421 aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata tcgtcactac
     6481 tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc tgtattccca
     6541 acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat tgaaagttgg
     6601 tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag attaagtatt
     6661 gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg gtttgatcat
     6721 caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat ttggtttaaa
     6781 tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac agaagaacga
     6841 atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg gaaagatctt
     6901 tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa agataaaaaa
     6961 atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg gaaaagttac
     7021 gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac tttcaatctc
     7081 agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac ttgtttcttt
     7141 gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc gcttgatcct
     7201 gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat ggatgcgtca
     7261 gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag cagagaatat
     7321 ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag gacgcagaaa
     7381 gggtgtgctt tgagcttaac cgaagcgttt gatagttgat atcctttgcc taattgtaag
     7441 tggaatgctt gcgacccagc tttcttgtac aaagtggtga tggggatcca ctagtgagct
     7501 catgcatgat cgaattagct tcaaaagcgc tctgaagttc ctatactttc tagagaatag
     7561 gaacttcgga ataggaactt caagatcccc tgattccctt tgtcaacagc aatggataat
     7621 tcgattaaca aatgcatggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag
     7681 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     7741 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     7801 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     7861 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     7921 caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     7981 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     8041 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     8101 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     8161 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     8221 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     8281 ccaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     8341 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     8401 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     8461 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     8521 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     8581 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     8641 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     8701 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     8761 aattgaaaaa ggaagagtat gaggatccaa catttccaat cacttaatta attattccat
     8821 tgagtaagtt tttaagcaca tcagcttcaa aagcgctctg aagttcctat actttctaga
     8881 gaataggaac ttcggaatag gtacttcaag atccccaatt cgatcgtccg ggccgcaagc
     8941 tcctagcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     9001 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctcaagc tagagagtca
     9061 ttaccccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     9121 actcatcgca gtctagcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     9181 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     9241 ctcagctaat taagctagct tatcgatacc gtcgacctcg aaccccacgc ccctctttaa
     9301 tacgacgggc aatttgcact tcagaaaatg aagagtttgc tttagccata acaaaagtcc
     9361 agtatgcttt ttcacagcat aactggactg atttcagttt acaactattc tgtctagttt
     9421 aagactttat tgtcatagtt tagatctatt ttgttcagtt taagacttta ttgtccgccc
     9481 acacccgctt acgcagggca tccatttatt actcaaccgt aaccgatttt gccaggttac
     9541 gcggctggtc ctctagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     9601 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     9661 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9721 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9781 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9841 caccgtcatc accgaaacgc gcga
//
