LOCUS       40924_43658       10805 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter capture vector pTn5.7luxT6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux : A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 10805)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_feature    complement(1276..1294)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     CDS             complement(1449..2876)
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     regulatory      complement(3019..3048)
                     /label=PS12
                     /note="PS12"
                     /regulatory_class="promoter"
     misc_feature    3055..3073
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     protein_bind    3083..3107
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3137..4576
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             4591..5511
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             5563..6642
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6660..7640
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             7822..8931
                     /label=LuxE
                     /note="LuxE"
     protein_bind    complement(8969..8993)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     9049..9096
                     /label=FRT
                     /note="FRT"
     protein_bind    complement(9049..9096)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9148..9176
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             9503..9736
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     protein_bind    complement(9801..9848)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(9894..9980)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(10083..10177)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(10205..10221)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10226..10424
                     /label=Tn7R
                     /note="Tn7R"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg
      181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg
      241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
      301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
      361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
      421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct
      481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc
      541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa
      601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
      661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
      721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
      781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
      841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg
      961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga
     1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     1261 tggcctgcaa ggcctagatg tgtataagag acagactctt cctttttcaa tattattgaa
     1321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcagat ctgcaggtcg
     1441 accgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
     1501 ttactttgca gggcttccca accttcccag agggcgcccc agctggcaat tccggttcgc
     1561 ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct
     1621 gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
     1681 ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct
     1741 tgcgccctga gtgcttgcgg cagcgtgaag ctttctctga gctgtaacag cctgaccgca
     1801 acaaacgaga ggatcgagac catccgctcc agattatccg gctcctccat gcgttgcctc
     1861 tcggctcctg ctccggtttt ccatgcctta tggaactcct cgatccgcca gcgatgggta
     1921 taaatgtcga tgacgcgcaa ggcttgggct agcgactcga ccggttcgct ggtcagcaac
     1981 aaccatttca acggggtctc acccttgggc gggttaatct cctcggccag caccgcgttg
     2041 agcgtgatat tcccctgttt tagcgtgatg cgcccactgc gcaggctcaa gctcgccttg
     2101 cgggctggtc gatttttacg tttaccgcgt ttatccacca cgcccttttg cggaatgctg
     2161 atctgatagc cacccaactc cggttggttc ttcagatggt cgtacagata caacccagac
     2221 tctacgtcct tgcgtgggtg cttggagcgc accacgaagc gctcgttatg cgccagtttg
     2281 tcctgcagat aagcatgaat atcggcttcg cggtcacaga ccgcaatcac gttgctcatc
     2341 atgctgccca tgcgtaaccg gctagttgcg gccgctgcca gccatttgcc actctccttt
     2401 tcatccgcat cggcagggtc atccgggcgc atccaccact cctgatgcag taatcctacg
     2461 gtgcggaatg tggtggcctc gagcaagaga acggagtgaa tccaccatcc gcgggattta
     2521 tcctgaatag agcccagctt gccaagctct tcggcgacct ggtggcgata actcaaagag
     2581 gtggtgtcct caatggccag cagttcggga aactcctgag ccaacttgac tgtttgcatg
     2641 gcgccagcct ttctgatcgc ctcggcagaa acgttgggat tgcggataaa tcggtaagcg
     2701 ccttcctggg cggcttcact accctctgat gagatgggta ttgatttacc agaatatttt
     2761 gccaattggg cggcgacgtt aaccaagcgg gcagtatggc gaggatcacc cagcgccgcc
     2821 gaagagaaca cagatttagc ccagtcggcc gcacgatgaa gagcagaagt tatcatgaac
     2881 gttaccatgt taggaggtca catggcagat cagatcctgg aaaacgggaa aggttccgtt
     2941 caggacgcta cttgtgtacg ggatcggtcg acggatccca agcttcttct agtaacggcc
     3001 gccagtgtgc tggaattcat gatattccga aaatcccaag tgagtcaaga attcctgtct
     3061 cttatacaca tctcacgcgg tgacaagttt gtacaaaaaa gcaggctgcg gtgcattaaa
     3121 tggatggcaa atatgaatga ctaaaaaaat ttcattcatt attaacggcc aggttgaaat
     3181 ctttcccgaa agtgatgatt tagtgcaatc cattaatttt ggtgataata gtgtttacct
     3241 gccaatattg aatgactctc atgtaaaaaa cattattgat tgtaatggaa ataacgaatt
     3301 acggttgcat aacattgtca attttctcta tacggtaggg caaagatgga aaaatgaaga
     3361 atactcaaga cgcaggacat acattcgtga cttaaaaaaa tatatgggat attcagaaga
     3421 aatggctaag ctagaggcca attggatatc tatgatttta tgttctaaag gcggccttta
     3481 tgatgttgta gaaaatgaac ttggttctcg ccatatcatg gatgaatggc tacctcagga
     3541 tgaaagttat gttcgggctt ttccgaaagg taaatctgta catctgttgg caggtaatgt
     3601 tccattatct gggatcatgt ctatattacg cgcaatttta actaagaatc agtgtattat
     3661 aaaaacatcg tcaaccgatc cttttaccgc taatgcatta gcgttaagtt ttattgatgt
     3721 agaccctaat catccgataa cgcgctcttt atctgttata tattggcccc accaaggtga
     3781 tacatcactc gcaaaagaaa ttatgcgaca tgcggatgtt attgtcgctt ggggagggcc
     3841 agatgcgatt aattgggcgg tagagcacgc gccatcttat gctgatgtga ttaaatttgg
     3901 ttctaaaaag agtctttgca ttatcgataa tcctgttgat ttgacgtccg cagcgacagg
     3961 tgcggctcat gatgtttgtt tttacgatca gcgagcttgt ttttctgccc aaaacatata
     4021 ttacatggga aatcattatg aggaatttaa gttagcgttg atagaaaaac ttaatctata
     4081 tgcgcatata ttaccgaatg ccaaaaaaga ttttgatgaa aaggcggcct attctttagt
     4141 tcaaaaagaa agcctgtttg ctggattaaa agtagaggtg gatattcatc aacgttggat
     4201 gattattgag tcaaatgcag gtgtggaatt taatcaacca cttggcagat gtgtgtacct
     4261 tcatcacgtc gataatattg agcaaatatt gccttatgtt caaaaaaata agacgcaaac
     4321 catatctatt tttccttggg agtcatcatt taaatatcga gatgcgttag cattaaaagg
     4381 tgcggaaagg attgtagaag caggaatgaa taacatattt cgagttggtg gatctcatga
     4441 cggaatgaga ccgttgcaac gattagtgac atatatttct catgaaaggc catctaacta
     4501 tacggctaag gatgttgcgg ttgaaataga acagactcga ttcctggaag aagataagtt
     4561 ccttgtattt gtcccataat aggtaaaagt atggaaaatg aatcaaaata taaaaccatc
     4621 gaccacgtta tttgtgttga aggaaataaa aaaattcatg tttgggaaac gctgccagaa
     4681 gaaaacagcc caaagagaaa gaatgccatt attattgcgt ctggttttgc ccgcaggatg
     4741 gatcattttg ctggtctggc ggaatattta tcgcggaatg gatttcatgt gatccgctat
     4801 gattcgcttc accacgttgg attgagttca gggacaattg atgaatttac aatgtctata
     4861 ggaaagcaga gcttgttagc agtggttgat tggttaacta cacgaaaaat aaataacttc
     4921 ggtatgttgg cttccagctt atctgcgcgg atagcttatg caagcctatc tgaaatcaat
     4981 gcttcgtttt taatcaccgc agtcggtgtt gttaacttaa gatattctct tgaaagagct
     5041 ttagggtttg attatctcag tctacccatt aatgaattgc cggataatct ggattttgaa
     5101 ggccataaat tgggtgctga agtctttgcg agagattgtc ttgattttgg ttgggaagat
     5161 ttagcttcta caattaataa catgatgtat cttgatatac cgtttattgc ttttactgca
     5221 aataacgata attgggtcaa gcaagatgaa gttatcacat tgttatcaaa tattcgtagt
     5281 aatcgatgca agatatattc tttgttagga agttcgcatg acttgagtga aaatttagtg
     5341 gtcctgcgca atttttatca atcggttacg aaagccgcta tcgcgatgga taatgatcat
     5401 ctggatattg atgttgatat tactgaaccg tcatttgaac atttaactat tgcgacagtc
     5461 aatgaacgcc gaatgagaat tgagattgaa aatcaagcaa tttctctgtc ttaaaatcta
     5521 ttgagatatt ctatcactca aatagcaata taaggactct ctatgaaatt tggaaacttt
     5581 ttgcttacat accaacctcc ccaattttct caaacagagg taatgaaacg tttggttaaa
     5641 ttaggtcgca tctctgagga gtgtggtttt gataccgtat ggttactgga gcatcatttc
     5701 acggagtttg gtttgcttgg taacccttat gtcgctgctg catatttact tggcgcgact
     5761 aaaaaattga atgtaggaac tgccgctatt gttcttccca cagcccatcc agtacgccaa
     5821 cttgaagatg tgaatttatt ggatcaaatg tcaaaaggac gatttcggtt tggtatttgc
     5881 cgagggcttt acaacaagga ctttcgcgta ttcggcacag atatgaataa cagtcgcgcc
     5941 ttagcggaat gctggtacgg gctgataaag aatggcatga cagagggata tatggaagct
     6001 gataatgaac atatcaagtt ccataaggta aaagtaaacc ccgcggcgta tagcagaggt
     6061 ggcgcaccgg tttatgtggt ggctgaatca gcttcgacga ctgagtgggc tgctcaattt
     6121 ggcctaccga tgatattaag ttggattata aatactaacg aaaagaaagc acaacttgag
     6181 ctttataatg aagtggctca agaatatggg cacgatattc ataatatcga ccattgctta
     6241 tcatatataa catctgtaga tcatgactca attaaagcga aagagatttg ccggaaattt
     6301 ctggggcatt ggtatgattc ttatgtgaat gctacgacta tttttgatga ttcagaccaa
     6361 acaagaggtt atgatttcaa taaagggcag tggcgtgact ttgtattaaa aggacataaa
     6421 gatactaatc gccgtattga ttacagttac gaaatcaatc ccgtgggaac gccgcaggaa
     6481 tgtattgaca taattcaaaa agacattgat gctacaggaa tatcaaatat ttgttgtgga
     6541 tttgaagcta atggaacagt agacgaaatt attgcttcca tgaagctctt ccagtctgat
     6601 gtcatgccat ttcttaaaga aaaacaacgt tcgctattat attagctaag gagaaagaaa
     6661 tgaaatttgg attgttcttc cttaacttca tcaattcaac aactgttcaa gaacaaagta
     6721 tagttcgtat gcaggaaata acggagtatg ttgataagtt gaattttgaa cagattttag
     6781 tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc tcctctgact gtttctggtt
     6841 ttctgctcgg tttaacagag aaaattaaaa ttggttcatt aaatcacatc attacaactc
     6901 atcatcctgt cgccatagcg gaggaagcgt gcttattgga tcagttaagt gaagggagat
     6961 ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg
     7021 ttgaatatca acagcaacta tttgaagagt gttatgaaat cattaacgat gctttaacaa
     7081 caggctattg taatccagat aacgattttt atagcttccc taaaatatct gtaaatcccc
     7141 atgcttatac gccaggcgga cctcggaaat atgtaacagc aaccagtcat catattgttg
     7201 agtgggcggc caaaaaaggt attcctctca tctttaagtg ggatgattct aatgatgtta
     7261 gatatgaata tgctgaaaga tataaagccg ttgcggataa atatgacgtt gacctatcag
     7321 agatagacca tcagttaatg atattagtta actataacga agatagtaat aaagctaaac
     7381 aagagacgcg tgcatttatt agtgattatg ttcttgaaat gcaccctaat gaaaatttcg
     7441 aaaataaact tgaagaaata attgcagaaa acgctgtcgg aaattatacg gagtgtataa
     7501 ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac
     7561 caatgaatga tttgatgagc caaaaaaatg taatcaatat tgttgatgat aatattaaga
     7621 agtaccacat ggaatatacc taatagattt cgagttgcag cgaggcggca agtgaacgaa
     7681 tccccaggag catagataac tatgtgactg gggtgagtga aagcagccaa caaagcagca
     7741 gcttgaaaga tgaagggtat aaaagagtat gacagcagtg ctgccatact ttctaatatt
     7801 atcttgagga gtaaaacagg tatgacttca tatgttgata aacaagaaat tacagcaagc
     7861 tcagaaattg atgatttgat tttttcgagc gatccattag tgtggtctta cgacgagcag
     7921 gaaaaaatca gaaagaaact tgtgcttgat gcatttcgta atcattataa acattgtcga
     7981 gaatatcgtc actactgtca ggcacacaaa gtagatgaca atattacgga aattgatgac
     8041 atacctgtat tcccaacatc ggtttttaag tttactcgct tattaacttc tcaggaaaac
     8101 gagattgaaa gttggtttac cagtagcggc acgaatggtt taaaaagtca ggtggcgcgt
     8161 gacagattaa gtattgagag actcttaggc tctgtgagtt atggcatgaa atatgttggt
     8221 agttggtttg atcatcaaat agaattagtc aatttgggac cagatagatt taatgctcat
     8281 aatatttggt ttaaatatgt tatgagtttg gtggaattgt tatatcctac gacatttacc
     8341 gtaacagaag aacgaataga ttttgttaaa acattgaata gtcttgaacg aataaaaaat
     8401 caagggaaag atctttgtct tattggttcg ccatacttta tttatttact ctgccattat
     8461 atgaaagata aaaaaatctc attttctgga gataaaagcc tttatatcat aaccggaggc
     8521 ggctggaaaa gttacgaaaa agaatctctg aaacgtgatg atttcaatca tcttttattt
     8581 gatactttca atctcagtga tattagtcag atccgagata tatttaatca agttgaactc
     8641 aacacttgtt tctttgagga tgaaatgcag cgtaaacatg ttccgccgtg ggtatatgcg
     8701 cgagcgcttg atcctgaaac gttgaaacct gtacctgatg gaacgccggg gttgatgagt
     8761 tatatggatg cgtcagcaac cagttatcca gcatttattg ttaccgatga tgtcgggata
     8821 attagcagag aatatggtaa gtatcccggc gtgctcgttg aaattttacg tcgcgtcaat
     8881 acgaggacgc agaaagggtg tgctttgagc ttaaccgaag cgtttgatag ttgatatcct
     8941 ttgcctaatt gtaagtggaa tgcttgcgac ccagctttct tgtacaaagt ggtgatgggg
     9001 atccactagt gagctcatgc atgatcgaat tagcttcaaa agcgctctga agttcctata
     9061 ctttctagag aataggaact tcggaatagg aacttcaaga tcccctgatt ccctttgtca
     9121 acagcaatgg ataattcacg aacccagttg acataagcct gttcggttcg taaactgtaa
     9181 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     9241 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctagcctc
     9301 gggcatccaa gctagctaag cgcgttacgc cgtgggtcga tgtttgatgt tatggaacag
     9361 caacgatgtt acgcagcagg gtagtcgccc taaaacaaag ttaggcagcc gttgtgctgg
     9421 tgctttctag tagttgttgt ggggtaggca gtcagagctc gatttgcttg tcgccataat
     9481 agattcacaa gaaggattcg acatgggtca aagtagcgat gaagccaacg ctcccgttgc
     9541 agggcagttt gcgcttcccc tgagtgccac ctttggctta ggggatcgcg tacgcaagaa
     9601 atctggtgcc gcttggcagg gtcaagtcgt cggttggtat tgcacaaaac tcactcctga
     9661 aggctatgcg gtcgagtccg aatcccaccc aggctcagtg caaatttatc ctgtggctgc
     9721 acttgaacgt gtggcctaag aattatctag gattattcca ttgagtaagt ttttaagcac
     9781 atcagcttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata
     9841 ggtacttcaa gatccccaat tcgatcgtcc gggccgcaag ctcctagcgg cggatttgtc
     9901 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     9961 tgagcctttc gttttatttg atgcctcaag ctagagagtc attaccccag gcgtttaagg
    10021 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtctagctt
    10081 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
    10141 agttctgagg tcattactgg atctatcaac aggagtccaa gctcagctaa ttaagctagc
    10201 ttatcgatac cgtcgacctc gaaccccacg cccctcttta atacgacggg caatttgcac
    10261 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
    10321 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
    10381 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
    10441 atccatttat tactcaaccg taaccgattt tgccaggtta cgcggctggt cctctagctg
    10501 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10561 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    10621 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
    10681 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10741 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10801 cgcga
//
