LOCUS       40924_43633       11265 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter capture vector pTn5.7luxK3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux : A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 11265)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11265)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_feature    complement(1276..1294)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     CDS             complement(1449..2876)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     IPISSEGSEAAQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    3031..3049
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             3082..4521
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             4536..5456
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             5508..6587
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             6605..7585
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             7767..8876
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     protein_bind    complement(8914..8938)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9368..10159
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(10261..10308)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(10354..10440)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(10543..10637)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(10665..10681)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10686..10884
                     /label=Tn7R
                     /note="Tn7R"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg
      181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg
      241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
      301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
      361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
      421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct
      481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc
      541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa
      601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
      661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
      721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
      781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
      841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg
      961 cagccgaacg accgagcgca gcgggtcagt gagcgaggaa gcggaagagc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga
     1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     1261 tggcctgcaa ggcctagatg tgtataagag acagactctt cctttttcaa tattattgaa
     1321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcagat ctgcaggtcg
     1441 accgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
     1501 ttactttgca gggcttccca accttcccag agggcgcccc agctggcaat tccggttcgc
     1561 ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct
     1621 gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
     1681 ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct
     1741 tgcgccctga gtgcttgcgg cagcgtgaag ctttctctga gctgtaacag cctgaccgca
     1801 acaaacgaga ggatcgagac catccgctcc agattatccg gctcctccat gcgttgcctc
     1861 tcggctcctg ctccggtttt ccatgcctta tggaactcct cgatccgcca gcgatgggta
     1921 taaatgtcga tgacgcgcaa ggcttgggct agcgactcga ccggttcgct ggtcagcaac
     1981 aaccatttca acggggtctc acccttgggc gggttaatct cctcggccag caccgcgttg
     2041 agcgtgatat tcccctgttt tagcgtgatg cgcccactgc gcaggctcaa gctcgccttg
     2101 cgggctggtc gatttttacg tttaccgcgt ttatccacca cgcccttttg cggaatgctg
     2161 atctgatagc cacccaactc cggttggttc ttcagatggt cgtacagata caacccagac
     2221 tctacgtcct tgcgtgggtg cttggagcgc accacgaagc gctcgttatg cgccagtttg
     2281 tcctgcagat aagcatgaat atcggcttcg cggtcacaga ccgcaatcac gttgctcatc
     2341 atgctgccca tgcgtaaccg gctagttgcg gccgctgcca gccatttgcc actctccttt
     2401 tcatccgcat cggcagggtc atccgggcgc atccaccact cctgatgcag taatcctacg
     2461 gtgcggaatg tggtggcctc gagcaagaga acggagtgaa cccaccatcc gcgggattta
     2521 tcctgaatag agcccagctt gccaagctct tcggcgacct ggtggcgata actcaaagag
     2581 gtggtgtcct caatggccag cagttcggga aactcctgag ccaacttgac tgtttgcatg
     2641 gcgccagcct ttctgatcgc ctcggcagaa acgttgggat tgcggataaa tcggtaagcg
     2701 ccttcctggg cggcttcact accctctgat gagatgggta ttgatttacc agaatatttt
     2761 gccaattggg cggcgacgtt aaccaagcgg gcagtacggc gaggatcacc cagcgccgcc
     2821 gaagagaaca cagatttagc ccagtcggcc gcacgatgaa gagcagaagt tatcatgaac
     2881 gttaccatgt taggaggtca catggaagat cagatcctgg aaaacgggaa aggttccgtt
     2941 caggacgcta cttgtgtacg ggatcggtcg acggatccca agcttcttct agtaacggcc
     3001 gccagtgtgc tggaattcgc ccttgaattc ctgtctctta tacacatctc acgcggtgca
     3061 ttaaatggat ggcaaatatg aatgactaaa aaaatttcat tcattattaa cggccaggtt
     3121 gaaatctttc ccgaaagtga tgatttagtg caatccatta attttggtga taatagtgtt
     3181 tacctgccaa tattgaatga ctctcatgta aaaaacatta ttgattgtaa tggaaataac
     3241 gaattacggt tgcataacat tgtcaatttt ctctatacgg tagggcaaag atggaaaaat
     3301 gaagaatact caagacgcag gacatacatt cgtgacttaa aaaaatatat gggatattca
     3361 gaagaaatgg ctaagctaga ggccaattgg atatctatga ttttatgttc taaaggcggc
     3421 ctttatgatg ttgtagaaaa tgaacttggt tctcgccata tcatggatga atggctacct
     3481 caggatgaaa gttatgttcg ggcttttccg aaaggtaaat ctgtacatct gttggcaggt
     3541 aatgttccat tatctgggat catgtctata ttacgcgcaa ttttaactaa gaatcagtgt
     3601 attataaaaa catcgtcaac cgatcctttt accgctaatg cattagcgtt aagttttatt
     3661 gatgtagacc ctaatcatcc gataacgcgc tctttatctg ttatatattg gccccaccaa
     3721 ggtgatacat cactcgcaaa agaaattatg cgacatgcgg atgttattgt cgcttgggga
     3781 gggccagatg cgattaattg ggcggtagag cacgcgccat cttatgctga tgtgattaaa
     3841 tttggttcta aaaagagtct ttgcattatc gataatcctg ttgatttgac gtccgcagcg
     3901 acaggtgcgg ctcatgatgt ttgtttttac gatcagcgag cttgtttttc tgcccaaaac
     3961 atatattaca tgggaaatca ttatgaggaa tttaagttag cgttgataga aaaacttaat
     4021 ctatatgcgc atatattacc gaatgccaaa aaagattttg atgaaaaggc ggcctattct
     4081 ttagttcaaa aagaaagcct gtttgctgga ttaaaagtag aggtggatat tcatcaacgt
     4141 tggatgatta ttgagtcaaa tgcaggtgtg gaatttaatc aaccacttgg cagatgtgtg
     4201 taccttcatc acgtcgataa tattgagcaa atattgcctt atgttcaaaa aaataagacg
     4261 caaaccatat ctatttttcc ttgggagtca tcatttaaat atcgagatgc gttagcatta
     4321 aaaggtgcgg aaaggattgt agaagcagga atgaataaca tatttcgagt tggtggatct
     4381 catgacggaa tgagaccgtt gcaacgatta gtgacatata tttctcatga aaggccatct
     4441 aactatacgg ctaaggatgt tgcggttgaa atagaacaga ctcgattcct ggaagaagat
     4501 aagttccttg tatttgtccc ataataggta aaagtatgga aaatgaatca aaatataaaa
     4561 ccatcgacca cgttatttgt gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc
     4621 cagaagaaaa cagcccaaag agaaagaatg ccattattat tgcgtctggt tttgcccgca
     4681 ggatggatca ttttgctggt ctggcggaat atttatcgcg gaatggattt catgtgatcc
     4741 gctatgattc gcttcaccac gttggattga gttcagggac aattgatgaa tttacaatgt
     4801 ctataggaaa gcagagcttg ttagcagtgg ttgattggtt aactacacga aaaataaata
     4861 acttcggtat gttggcttcc agcttatctg cgcggatagc ttatgcaagc ctatctgaaa
     4921 tcaatgcttc gtttttaatc accgcagtcg gtgttgttaa cttaagatat tctcttgaaa
     4981 gagctttagg gtttgattat ctcagtctac ccattaatga attgccggat aatctggatt
     5041 ttgaaggcca taaattgggt gctgaagtct ttgcgagaga ttgtcttgat tttggttggg
     5101 aagatttagc ttctacaatt aataacatga tgtatcttga tataccgttt attgctttta
     5161 ctgcaaataa cgataattgg gtcaagcaag atgaagttat cacattgtta tcaaatattc
     5221 gtagtaatcg atgcaagata tattctttgt taggaagttc gcatgacttg agtgaaaatt
     5281 tagtggtcct gcgcaatttt tatcaatcgg ttacgaaagc cgctatcgcg atggataatg
     5341 atcatctgga tattgatgtt gatattactg aaccgtcatt tgaacattta actattgcga
     5401 cagtcaatga acgccgaatg agaattgaga ttgaaaatca agcaatttct ctgtcttaaa
     5461 atctattgag atattctatc actcaaatag caatataagg actctctatg aaatttggaa
     5521 actttttgct tacataccaa cctccccaat tttctcaaac agaggtaatg aaacgtttgg
     5581 ttaaattagg tcgcatctct gaggagtgtg gttttgatac cgtatggtta ctggagcatc
     5641 atttcacgga gtttggtttg cttggtaacc cttatgtcgc tgctgcatat ttacttggcg
     5701 cgactaaaaa attgaatgta ggaactgccg ctattgttct tcccacagcc catccagtac
     5761 gccaacttga agatgtgaat ttattggatc aaatgtcaaa aggacgattt cggtttggta
     5821 tttgccgagg gctttacaac aaggactttc gcgtattcgg cacagatatg aataacagtc
     5881 gcgccttagc ggaatgctgg tacgggctga taaagaatgg catgacagag ggatatatgg
     5941 aagctgataa tgaacatatc aagttccata aggtaaaagt aaaccccgcg gcgtatagca
     6001 gaggtggcgc accggtttat gtggtggctg aatcagcttc gacgactgag tgggctgctc
     6061 aatttggcct accgatgata ttaagttgga ttataaatac taacgaaaag aaagcacaac
     6121 ttgagcttta taatgaagtg gctcaagaat atgggcacga tattcataat atcgaccatt
     6181 gcttatcata tataacatct gtagatcatg actcaattaa agcgaaagag atttgccgga
     6241 aatttctggg gcattggtat gattcttatg tgaatgctac gactattttt gatgattcag
     6301 accaaacaag aggttatgat ttcaataaag ggcagtggcg tgactttgta ttaaaaggac
     6361 ataaagatac taatcgccgt attgattaca gttacgaaat caatcccgtg ggaacgccgc
     6421 aggaatgtat tgacataatt caaaaagaca ttgatgctac aggaatatca aatatttgtt
     6481 gtggatttga agctaatgga acagtagacg aaattattgc ttccatgaag ctcttccagt
     6541 ctgatgtcat gccatttctt aaagaaaaac aacgttcgct attatattag ctaaggagaa
     6601 agaaatgaaa tttggattgt tcttccttaa cttcatcaat tcaacaactg ttcaagaaca
     6661 aagtatagtt cgtatgcagg aaataacgga gtatgttgat aagttgaatt ttgaacagat
     6721 tttagtgtat gaaaatcatt tttcagataa tggtgttgtc ggcgctcctc tgactgtttc
     6781 tggttttctg ctcggtttaa cagagaaaat taaaattggt tcattaaatc acatcattac
     6841 aactcatcat cctgtcgcca tagcggagga agcgtgctta ttggatcagt taagtgaagg
     6901 gagatttatt ttagggttta gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg
     6961 cccggttgaa tatcaacagc aactatttga agagtgttat gaaatcatta acgatgcttt
     7021 aacaacaggc tattgtaatc cagataacga tttttatagc ttccctaaaa tatctgtaaa
     7081 tccccatgct tatacgccag gcggacctcg gaaatatgta acagcaacca gtcatcatat
     7141 tgttgagtgg gcggccaaaa aaggtattcc tctcatcttt aagtgggatg attctaatga
     7201 tgttagatat gaatatgctg aaagatataa agccgttgcg gataaatatg acgttgacct
     7261 atcagagata gaccatcagt taatgatatt agttaactat aacgaagata gtaataaagc
     7321 taaacaagag acgcgtgcat ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa
     7381 tttcgaaaat aaacttgaag aaataattgc agaaaacgct gtcggaaatt atacggagtg
     7441 tataactgcg gctaagttgg caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt
     7501 tgaaccaatg aatgatttga tgagccaaaa aaatgtaatc aatattgttg atgataatat
     7561 taagaagtac cacatggaat atacctaata gatttcgagt tgcagcgagg cggcaagtga
     7621 acgaatcccc aggagcatag ataactatgt gactggggtg agtgaaagca gccaacaaag
     7681 cagcagcttg aaagatgaag ggtataaaag agtatgacag cagtgctgcc atactttcta
     7741 atattatctt gaggagtaaa acaggtatga cttcatatgt tgataaacaa gaaattacag
     7801 caagctcaga aattgatgat ttgatttttt cgagcgatcc attagtgtgg tcttacgacg
     7861 agcaggaaaa aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt
     7921 gtcgagaata tcgtcactac tgtcaggcac acaaagtaga tgacaatatt acggaaattg
     7981 atgacatacc tgtattccca acatcggttt ttaagtttac tcgcttatta acttctcagg
     8041 aaaacgagat tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg
     8101 cgcgtgacag attaagtatt gagagactct taggctctgt gagttatggc atgaaatatg
     8161 ttggtagttg gtttgatcat caaatagaat tagtcaattt gggaccagat agatttaatg
     8221 ctcataatat ttggtttaaa tatgttatga gtttggtgga attgttatat cctacgacat
     8281 ttaccgtaac agaagaacga atagattttg ttaaaacatt gaatagtctt gaacgaataa
     8341 aaaatcaagg gaaagatctt tgtcttattg gttcgccata ctttatttat ttactctgcc
     8401 attatatgaa agataaaaaa atctcatttt ctggagataa aagcctttat atcataaccg
     8461 gaggcggctg gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt
     8521 tatttgatac tttcaatctc agtgatatta gtcagatccg agatatattt aatcaagttg
     8581 aactcaacac ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat
     8641 atgcgcgagc gcttgatcct gaaacgttga aacctgtacc tgatggaacg ccggggttga
     8701 tgagttatat ggatgcgtca gcaaccagtt atccagcatt tattgttacc gatgatgtcg
     8761 ggataattag cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg
     8821 tcaatacgag gacgcagaaa gggtgtgctt tgagcttaac cgaagcgttt gatagttgat
     8881 atcctttgcc taattgtaag tggaatgctt gcgacccagc tttcttgtac aaagtggtga
     8941 tggggatcct ctagagaata ggaacttcgg aataggaact tcaagatccc ctgattccct
     9001 ttgtcaacag caatggataa ttcgattaac aaatgcatgg cgcaagggct gctaaaggaa
     9061 gcggaacacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
     9121 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
     9181 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
     9241 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
     9301 gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc
     9361 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     9421 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     9481 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     9541 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     9601 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     9661 ggggcaggat ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga
     9721 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     9781 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     9841 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     9901 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     9961 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    10021 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    10081 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    10141 ccttcttgac gagttcttct gaattgaaaa aggaagagta tgaggatcca acatttccaa
    10201 tcacttaatt aattattcca ttgagtaagt ttttaagcac atcagcttca aaagcgctct
    10261 gaagttccta tactttctag agaataggaa cttcggaata ggtacttcaa gatccccaat
    10321 tcgatcgtcc gggccgcaag ctcctagcgg cggatttgtc ctactcagga gagcgttcac
    10381 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
    10441 atgcctcaag ctagagagtc attaccccag gcgtttaagg gcaccaataa ctgccttaaa
    10501 aaaattacgc cccgccctgc cactcatcgc agtctagctt ggattctcac caataaaaaa
    10561 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
    10621 atctatcaac aggagtccaa gctcagctaa ttaagctagc ttatcgatac cgtcgacctc
    10681 gaaccccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    10741 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    10801 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
    10861 ttaagacttt attgtccgcc cacacccgct tacgcagggc atccatttat tactcaaccg
    10921 taaccgattt tgccaggtta cgcggctggt cctctagctg gcgtaatagc gaagaggccc
    10981 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
    11041 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
    11101 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
    11161 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
    11221 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga
//
