LOCUS 40924_43628 11021 bp DNA circular SYN 18-DEC-2018 DEFINITION Promoter capture vector pTn5.7luxG6, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11021) AUTHORS Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP. TITLE Tn5/7 lux : A versatile vector for the location, capture, and utilization of Gram negative promoters JOURNAL Unpublished REFERENCE 2 (bases 1 to 11021) AUTHORS Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP. TITLE Direct Submission JOURNAL Submitted (10-DEC-2012) Microbiology Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, Campus Delivery 0922, Fort Collins, CO 80523-0922, USA REFERENCE 3 (bases 1 to 11021) TITLE Direct Submission REFERENCE 4 (bases 1 to 11021) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (10-DEC-2012) Microbiology Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, Campus Delivery 0922, Fort Collins, CO 80523-0922, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..11021 /mol_type="other DNA" /organism="synthetic DNA construct" oriT 365..473 /label=oriT /note="incP origin of transfer" rep_origin 496..880 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" mobile_element 1096..1261 /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)" misc_feature complement(1276..1294) /label=Tn5 ME /note="hyperactive mosaic end for Tn5 transposase recognition (Reznikoff et al., 2004)" promoter complement(1295..1399) /label=AmpR promoter CDS complement(1449..2876) /label=Tn5 transposase /note="transposase from the bacterial Tn5 transposon (Reznikoff, 1993)" regulatory complement(3019..3048) /label=PS12 /note="PS12" /regulatory_class="promoter" misc_feature 3055..3073 /label=Tn5 ME /note="hyperactive mosaic end for Tn5 transposase recognition (Reznikoff et al., 2004)" protein_bind 3083..3107 /label=attB1 /note="recombination site for the Gateway(R) BP reaction" CDS 3137..4576 /label=LuxC /note="LuxC fatty acid reductase" CDS 4591..5511 /label=LuxD /note="LuxD acyltransferase" CDS 5563..6642 /label=LuxA /note="LuxA luciferase subunit" CDS 6660..7640 /label=LuxB /note="LuxB luciferase subunit" CDS 7822..8931 /label=LuxE /note="LuxE" protein_bind complement(8969..8993) /label=attB2 /note="recombination site for the Gateway(R) BP reaction" misc_recomb 9049..9096 /label=FRT /note="FRT" protein_bind complement(9049..9096) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 9137..9165 /label=Pc promoter /note="class 1 integron promoter" CDS 9354..9884 /label=GmR /note="gentamycin acetyltransferase" protein_bind complement(10017..10064) /label=FRT /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator complement(10110..10196) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator complement(10299..10393) /label=lambda t0 terminator /note="transcription terminator from phage lambda" primer_bind complement(10421..10437) /label=KS primer /note="common sequencing primer, one of multiple similar variants" misc_feature 10442..10640 /label=Tn7R /note="Tn7R" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg 181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg 241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac 301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc 361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc 421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct 481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc 541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa 601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg 661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct 721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt 781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca 841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta 901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg 961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga 1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga 1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt 1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt 1261 tggcctgcaa ggcctagatg tgtataagag acagactctt cctttttcaa tattattgaa 1321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 1381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcagat ctgcaggtcg 1441 accgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt 1501 ttactttgca gggcttccca accttcccag agggcgcccc agctggcaat tccggttcgc 1561 ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct 1621 gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc 1681 ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct 1741 tgcgccctga gtgcttgcgg cagcgtgaag ctttctctga gctgtaacag cctgaccgca 1801 acaaacgaga ggatcgagac catccgctcc agattatccg gctcctccat gcgttgcctc 1861 tcggctcctg ctccggtttt ccatgcctta tggaactcct cgatccgcca gcgatgggta 1921 taaatgtcga tgacgcgcaa ggcttgggct agcgactcga ccggttcgct ggtcagcaac 1981 aaccatttca acggggtctc acccttgggc gggttaatct cctcggccag caccgcgttg 2041 agcgtgatat tcccctgttt tagcgtgatg cgcccactgc gcaggctcaa gctcgccttg 2101 cgggctggtc gatttttacg tttaccgcgt ttatccacca cgcccttttg cggaatgctg 2161 atctgatagc cacccaactc cggttggttc ttcagatggt cgtacagata caacccagac 2221 tctacgtcct tgcgtgggtg cttggagcgc accacgaagc gctcgttatg cgccagtttg 2281 tcctgcagat aagcatgaat atcggcttcg cggtcacaga ccgcaatcac gttgctcatc 2341 atgctgccca tgcgtaaccg gctagttgcg gccgctgcca gccatttgcc actctccttt 2401 tcatccgcat cggcagggtc atccgggcgc atccaccact cctgatgcag taatcctacg 2461 gtgcggaatg tggtggcctc gagcaagaga acggagtgaa tccaccatcc gcgggattta 2521 tcctgaatag agcccagctt gccaagctct tcggcgacct ggtggcgata actcaaagag 2581 gtggtgtcct caatggccag cagttcggga aactcctgag ccaacttgac tgtttgcatg 2641 gcgccagcct ttctgatcgc ctcggcagaa acgttgggat tgcggataaa tcggtaagcg 2701 ccttcctggg cggcttcact accctctgat gagatgggta ttgatttacc agaatatttt 2761 gccaattggg cggcgacgtt aaccaagcgg gcagtatggc gaggatcacc cagcgccgcc 2821 gaagagaaca cagatttagc ccagtcggcc gcacgatgaa gagcagaagt tatcatgaac 2881 gttaccatgt taggaggtca catggcagat cagatcctgg aaaacgggaa aggttccgtt 2941 caggacgcta cttgtgtacg ggatcggtcg acggatccca agcttcttct agtaacggcc 3001 gccagtgtgc tggaattcat gatattccga aaatcccaag tgagtcaaga attcctgtct 3061 cttatacaca tctcacgcgg tgacaagttt gtacaaaaaa gcaggctgcg gtgcattaaa 3121 tggatggcaa atatgaatga ctaaaaaaat ttcattcatt attaacggcc aggttgaaat 3181 ctttcccgaa agtgatgatt tagtgcaatc cattaatttt ggtgataata gtgtttacct 3241 gccaatattg aatgactctc atgtaaaaaa cattattgat tgtaatggaa ataacgaatt 3301 acggttgcat aacattgtca attttctcta tacggtaggg caaagatgga aaaatgaaga 3361 atactcaaga cgcaggacat acattcgtga cttaaaaaaa tatatgggat attcagaaga 3421 aatggctaag ctagaggcca attggatatc tatgatttta tgttctaaag gcggccttta 3481 tgatgttgta gaaaatgaac ttggttctcg ccatatcatg gatgaatggc tacctcagga 3541 tgaaagttat gttcgggctt ttccgaaagg taaatctgta catctgttgg caggtaatgt 3601 tccattatct gggatcatgt ctatattacg cgcaatttta actaagaatc agtgtattat 3661 aaaaacatcg tcaaccgatc cttttaccgc taatgcatta gcgttaagtt ttattgatgt 3721 agaccctaat catccgataa cgcgctcttt atctgttata tattggcccc accaaggtga 3781 tacatcactc gcaaaagaaa ttatgcgaca tgcggatgtt attgtcgctt ggggagggcc 3841 agatgcgatt aattgggcgg tagagcacgc gccatcttat gctgatgtga ttaaatttgg 3901 ttctaaaaag agtctttgca ttatcgataa tcctgttgat ttgacgtccg cagcgacagg 3961 tgcggctcat gatgtttgtt tttacgatca gcgagcttgt ttttctgccc aaaacatata 4021 ttacatggga aatcattatg aggaatttaa gttagcgttg atagaaaaac ttaatctata 4081 tgcgcatata ttaccgaatg ccaaaaaaga ttttgatgaa aaggcggcct attctttagt 4141 tcaaaaagaa agcctgtttg ctggattaaa agtagaggtg gatattcatc aacgttggat 4201 gattattgag tcaaatgcag gtgtggaatt taatcaacca cttggcagat gtgtgtacct 4261 tcatcacgtc gataatattg agcaaatatt gccttatgtt caaaaaaata agacgcaaac 4321 catatctatt tttccttggg agtcatcatt taaatatcga gatgcgttag cattaaaagg 4381 tgcggaaagg attgtagaag caggaatgaa taacatattt cgagttggtg gatctcatga 4441 cggaatgaga ccgttgcaac gattagtgac atatatttct catgaaaggc catctaacta 4501 tacggctaag gatgttgcgg ttgaaataga acagactcga ttcctggaag aagataagtt 4561 ccttgtattt gtcccataat aggtaaaagt atggaaaatg aatcaaaata taaaaccatc 4621 gaccacgtta tttgtgttga aggaaataaa aaaattcatg tttgggaaac gctgccagaa 4681 gaaaacagcc caaagagaaa gaatgccatt attattgcgt ctggttttgc ccgcaggatg 4741 gatcattttg ctggtctggc ggaatattta tcgcggaatg gatttcatgt gatccgctat 4801 gattcgcttc accacgttgg attgagttca gggacaattg atgaatttac aatgtctata 4861 ggaaagcaga gcttgttagc agtggttgat tggttaacta cacgaaaaat aaataacttc 4921 ggtatgttgg cttccagctt atctgcgcgg atagcttatg caagcctatc tgaaatcaat 4981 gcttcgtttt taatcaccgc agtcggtgtt gttaacttaa gatattctct tgaaagagct 5041 ttagggtttg attatctcag tctacccatt aatgaattgc cggataatct ggattttgaa 5101 ggccataaat tgggtgctga agtctttgcg agagattgtc ttgattttgg ttgggaagat 5161 ttagcttcta caattaataa catgatgtat cttgatatac cgtttattgc ttttactgca 5221 aataacgata attgggtcaa gcaagatgaa gttatcacat tgttatcaaa tattcgtagt 5281 aatcgatgca agatatattc tttgttagga agttcgcatg acttgagtga aaatttagtg 5341 gtcctgcgca atttttatca atcggttacg aaagccgcta tcgcgatgga taatgatcat 5401 ctggatattg atgttgatat tactgaaccg tcatttgaac atttaactat tgcgacagtc 5461 aatgaacgcc gaatgagaat tgagattgaa aatcaagcaa tttctctgtc ttaaaatcta 5521 ttgagatatt ctatcactca aatagcaata taaggactct ctatgaaatt tggaaacttt 5581 ttgcttacat accaacctcc ccaattttct caaacagagg taatgaaacg tttggttaaa 5641 ttaggtcgca tctctgagga gtgtggtttt gataccgtat ggttactgga gcatcatttc 5701 acggagtttg gtttgcttgg taacccttat gtcgctgctg catatttact tggcgcgact 5761 aaaaaattga atgtaggaac tgccgctatt gttcttccca cagcccatcc agtacgccaa 5821 cttgaagatg tgaatttatt ggatcaaatg tcaaaaggac gatttcggtt tggtatttgc 5881 cgagggcttt acaacaagga ctttcgcgta ttcggcacag atatgaataa cagtcgcgcc 5941 ttagcggaat gctggtacgg gctgataaag aatggcatga cagagggata tatggaagct 6001 gataatgaac atatcaagtt ccataaggta aaagtaaacc ccgcggcgta tagcagaggt 6061 ggcgcaccgg tttatgtggt ggctgaatca gcttcgacga ctgagtgggc tgctcaattt 6121 ggcctaccga tgatattaag ttggattata aatactaacg aaaagaaagc acaacttgag 6181 ctttataatg aagtggctca agaatatggg cacgatattc ataatatcga ccattgctta 6241 tcatatataa catctgtaga tcatgactca attaaagcga aagagatttg ccggaaattt 6301 ctggggcatt ggtatgattc ttatgtgaat gctacgacta tttttgatga ttcagaccaa 6361 acaagaggtt atgatttcaa taaagggcag tggcgtgact ttgtattaaa aggacataaa 6421 gatactaatc gccgtattga ttacagttac gaaatcaatc ccgtgggaac gccgcaggaa 6481 tgtattgaca taattcaaaa agacattgat gctacaggaa tatcaaatat ttgttgtgga 6541 tttgaagcta atggaacagt agacgaaatt attgcttcca tgaagctctt ccagtctgat 6601 gtcatgccat ttcttaaaga aaaacaacgt tcgctattat attagctaag gagaaagaaa 6661 tgaaatttgg attgttcttc cttaacttca tcaattcaac aactgttcaa gaacaaagta 6721 tagttcgtat gcaggaaata acggagtatg ttgataagtt gaattttgaa cagattttag 6781 tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc tcctctgact gtttctggtt 6841 ttctgctcgg tttaacagag aaaattaaaa ttggttcatt aaatcacatc attacaactc 6901 atcatcctgt cgccatagcg gaggaagcgt gcttattgga tcagttaagt gaagggagat 6961 ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg 7021 ttgaatatca acagcaacta tttgaagagt gttatgaaat cattaacgat gctttaacaa 7081 caggctattg taatccagat aacgattttt atagcttccc taaaatatct gtaaatcccc 7141 atgcttatac gccaggcgga cctcggaaat atgtaacagc aaccagtcat catattgttg 7201 agtgggcggc caaaaaaggt attcctctca tctttaagtg ggatgattct aatgatgtta 7261 gatatgaata tgctgaaaga tataaagccg ttgcggataa atatgacgtt gacctatcag 7321 agatagacca tcagttaatg atattagtta actataacga agatagtaat aaagctaaac 7381 aagagacgcg tgcatttatt agtgattatg ttcttgaaat gcaccctaat gaaaatttcg 7441 aaaataaact tgaagaaata attgcagaaa acgctgtcgg aaattatacg gagtgtataa 7501 ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac 7561 caatgaatga tttgatgagc caaaaaaatg taatcaatat tgttgatgat aatattaaga 7621 agtaccacat ggaatatacc taatagattt cgagttgcag cgaggcggca agtgaacgaa 7681 tccccaggag catagataac tatgtgactg gggtgagtga aagcagccaa caaagcagca 7741 gcttgaaaga tgaagggtat aaaagagtat gacagcagtg ctgccatact ttctaatatt 7801 atcttgagga gtaaaacagg tatgacttca tatgttgata aacaagaaat tacagcaagc 7861 tcagaaattg atgatttgat tttttcgagc gatccattag tgtggtctta cgacgagcag 7921 gaaaaaatca gaaagaaact tgtgcttgat gcatttcgta atcattataa acattgtcga 7981 gaatatcgtc actactgtca ggcacacaaa gtagatgaca atattacgga aattgatgac 8041 atacctgtat tcccaacatc ggtttttaag tttactcgct tattaacttc tcaggaaaac 8101 gagattgaaa gttggtttac cagtagcggc acgaatggtt taaaaagtca ggtggcgcgt 8161 gacagattaa gtattgagag actcttaggc tctgtgagtt atggcatgaa atatgttggt 8221 agttggtttg atcatcaaat agaattagtc aatttgggac cagatagatt taatgctcat 8281 aatatttggt ttaaatatgt tatgagtttg gtggaattgt tatatcctac gacatttacc 8341 gtaacagaag aacgaataga ttttgttaaa acattgaata gtcttgaacg aataaaaaat 8401 caagggaaag atctttgtct tattggttcg ccatacttta tttatttact ctgccattat 8461 atgaaagata aaaaaatctc attttctgga gataaaagcc tttatatcat aaccggaggc 8521 ggctggaaaa gttacgaaaa agaatctctg aaacgtgatg atttcaatca tcttttattt 8581 gatactttca atctcagtga tattagtcag atccgagata tatttaatca agttgaactc 8641 aacacttgtt tctttgagga tgaaatgcag cgtaaacatg ttccgccgtg ggtatatgcg 8701 cgagcgcttg atcctgaaac gttgaaacct gtacctgatg gaacgccggg gttgatgagt 8761 tatatggatg cgtcagcaac cagttatcca gcatttattg ttaccgatga tgtcgggata 8821 attagcagag aatatggtaa gtatcccggc gtgctcgttg aaattttacg tcgcgtcaat 8881 acgaggacgc agaaagggtg tgctttgagc ttaaccgaag cgtttgatag ttgatatcct 8941 ttgcctaatt gtaagtggaa tgcttgcgac ccagctttct tgtacaaagt ggtgatgggg 9001 atccactagt gagctcatgc atgatcgaat tagcttcaaa agcgctctga agttcctata 9061 ctttctagag aataggaact tcggaatagg aacttcaaga tcccctgatt ccctttgtca 9121 acagcaatgg atcgaattga cataagcctg ttcggttcgt aaactgtaat gcaagtagcg 9181 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt 9241 ggcggttttc atggcttgtt atgactgttt ttttgtacag tctatgcctc gggcatccaa 9301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac 9361 gcagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta ggtggctcaa 9421 gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc atgcgggctg 9481 ctcttgatct tttcggtcgt gagttcggag acgtagccac ctactcccaa catcagccgg 9541 actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt gctgccttcg 9601 accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaggttt gagcagccgc 9661 gtagtgagat ctatatctat gatctcgcag tctccggcga gcaccggagg cagggcattg 9721 ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct tatgtgatct 9781 acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag ttgggcatac 9841 gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaacaa ttcgttcaag 9901 ccgagatcgg cttcccggcc gcggagttgt tcggtaaatt gtcacaacgc cgcggccaat 9961 tcgatctaga attccattga gtaagttttt aagcacatca gcttcaaaag cgctctgaag 10021 ttcctatact ttctagagaa taggaacttc ggaataggta cttcaagatc cccaattcga 10081 tcgtccgggc cgcaagctcc tagcggcgga tttgtcctac tcaggagagc gttcaccgac 10141 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc 10201 ctcaagctag agagtcatta ccccaggcgt ttaagggcac caataactgc cttaaaaaaa 10261 ttacgccccg ccctgccact catcgcagtc tagcttggat tctcaccaat aaaaaacgcc 10321 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct 10381 atcaacagga gtccaagctc agctaattaa gctagcttat cgataccgtc gacctcgaac 10441 cccacgcccc tctttaatac gacgggcaat ttgcacttca gaaaatgaag agtttgcttt 10501 agccataaca aaagtccagt atgctttttc acagcataac tggactgatt tcagtttaca 10561 actattctgt ctagtttaag actttattgt catagtttag atctattttg ttcagtttaa 10621 gactttattg tccgcccaca cccgcttacg cagggcatcc atttattact caaccgtaac 10681 cgattttgcc aggttacgcg gctggtcctc tagctggcgt aatagcgaag aggcccgcac 10741 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt 10801 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg 10861 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg 10921 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 10981 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a //