LOCUS       40924_43363        3314 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing the Fluorescent Timer in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             289..966
                     /codon_start=1
                     /label=Timer
                     /note="green-to-red fluorescent timer derivative of DsRed 
                     (Terskikh et al., 2000)"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     promoter        1409..1513
                     /label=AmpR promoter
     CDS             1514..2371
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2545..3133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgcgctcc
      301 tccaagaacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac
      361 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg ccacaacacc
      421 gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
      481 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
      541 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      601 gtggcgaccg tgacccagga ctcctccctg caggacggct gcttcatcta caaggtgaag
      661 ttcatcggcg tgaacttccc ctccgacggc cccgtgatgc agaagaagac catgggctgg
      721 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag
      781 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
      841 aagaagcccg tgcagctgcc cggctactac tacgtggaca ccaagctgga catcacctcc
      901 cacaacgagg actacaccat cgtggagcag tacgagcgca ccgagggccg ccaccacctg
      961 ttcctgtagc ggccgcgact ctagaattcc aactgagcgc cggtcgctac cattaccaac
     1021 ttgtctggtg tcaaaaataa taggcctact agtcggccgt acgggccctt tcgtctcgcg
     1081 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     1141 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     1201 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     1261 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     1321 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1681 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1921 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2041 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2341 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2401 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2461 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3121 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3181 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3241 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3301 cgaggaagcg gaag
//
