LOCUS       40924_43358        4110 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless Fluorescent Timer reporter vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Can be used to monitor transcription from a promoter inserted into 
            the MCS.
FEATURES             Location/Qualifiers
     source          1..4110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             97..774
                     /codon_start=1
                     /label=Timer
                     /note="green-to-red fluorescent timer derivative of DsRed 
                     (Terskikh et al., 2000)"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    898..1019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1026..1481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1508..1612
                     /label=AmpR promoter
     promoter        1614..1971
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2006..2797
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3032..3079
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3408..3996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgcgctcctc caagaacgtc
      121 atcaaggagt tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg ccacgagttc
      181 gagatcgagg gcgagggcga gggccgcccc tacgagggcc acaacaccgt gaagctgaag
      241 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac
      301 ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
      361 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg
      421 acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt catcggcgtg
      481 aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctccacc
      541 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg
      601 aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg
      661 cagctgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
      721 tacaccatcg tggagcagta cgagcgcacc gagggccgcc accacctgtt cctgtagcgg
      781 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
      841 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      901 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      961 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     1021 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     1081 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     1141 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     1201 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     1261 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     1321 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     1381 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1441 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     1501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     1621 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     1681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     1741 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     1801 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1921 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     1981 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     2041 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     2101 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     2161 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     2221 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     2281 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     2341 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     2401 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     2461 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     2521 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     2581 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     2641 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     2701 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     2761 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     2821 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     2881 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     2941 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     3001 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     3061 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     3121 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     3181 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     3241 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3301 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3421 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3481 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3541 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3601 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3661 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3721 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3781 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3841 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3901 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3961 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4021 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4081 tctgtggata accgtattac cgccatgcat
//
