LOCUS       Exported                5014 bp DNA     circular SYN 10-OCT-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5014)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(574..1419)
                     /codon_start=1
                     /label=AraC
                     /note="arabinose operon transcriptional regulator AraC"
                     /translation="MAETQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGEGVINNNGEQFVCRPGDILLFPPGEIHHYGRHPDASEWYHQWVYFRPRAYW
                     QEWLTWPTIFAQTGFFRPDEARQPHFSELFGQIISAGQGEGRYSELLAINLLEQLLLRR
                     MAVINESLHPPMDSRVRDACQYISDHLADSHFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     "
     CDS             1891..3186
                     /codon_start=1
                     /label=Trigger Factor
                     /note="ribosome-associated chaperone from E. coli (Hoffmann
                     et al., 2010)"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFR
                     KGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFT
                     YSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDR
                     VTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEYH
                     AENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSA
                     IRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQ
                     AKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMR
                     NVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     rep_origin      3622..4167
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        4693..4795
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             join(4796..5014,1..438)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa tagttacggc
      541 ttatgacatc tttgtggaca catcattcac tttttattca catccggccc tgaactcgct
      601 aggacttgcc ccggtgcatt ttttaaatac ccgcgaaaaa tagagctgat cgtcaaatcc
      661 aacattgcgc ccaacggtcg ctatcggcat tcgcgtagtg ctaagcagaa gtttcgcctg
      721 gctgatacgc tgatcttcgc gccagctcaa tacgctaatg cctaactgct ggcggaacag
      781 atgtgataac cgggagggcg acaggcagac atgctgggcg acgctggcga tatcaaaatg
      841 gctgtccgcc agatggtcgc tgatatactg gcaggcatcg cgcacacggc tatccatcgg
      901 cgggtgcaac gactcattaa ttaccgccat acgtctgagc aacaactgct ccagcagatt
      961 gatcgccagt agctcagaat agcgaccttc cccttgcccg gcgctgatga tctgcccgaa
     1021 cagttcgctg aaatgcggct ggcgcgcctc gtccgggcgg aaaaatcctg tctgggcaaa
     1081 gattgtcggc caggtcagcc actcctgcca gtaggcgcga ggccggaaat aaacccactg
     1141 gtgataccac tcgctggcgt ccggatgccg tccatagtga tgaatctcgc ccggcggaaa
     1201 caataatata tcgccaggcc gacagacaaa ctgctcgcca ttattattaa tgacgccctc
     1261 tccgcggatg gtcaggttaa gaatatatcc cttcatgccc aacggacgat cgataaaaaa
     1321 atccagatat ccattcgctt caattggcgt cagcccggcg accagatggg cattaaatga
     1381 atatcccggc aatagcggat cattttgcgt ttcagccatg atttctctac cccccgatgt
     1441 tcagagaaga aacaaattgt ccatatcgac caggacgaca gagcttccgt ctccgcaaga
     1501 ctttgcgctt gatgaaagca cgtatcaacc ccgcttgtga aaagcgcttt gtaacaaaag
     1561 cgtacagttc aggcgataaa attaagtaac agaagtgtct ataactatgg ctggaatgtc
     1621 cacattgaat atttgcacag cgtcacactt tgcaaagcat tagcattttt gtccataaga
     1681 ttagcggatc ctgcctgacg gtttttgccg cgactctcta taatttctcc atacctgttt
     1741 ttctggatgg agtaagacca tggcctttcg ggatgattct ggtaacaggg aatgtgattg
     1801 attataagaa catcccggtt ccgcgaagcc aacaacctgt gcttgcgggg taagagttga
     1861 ccgagcactg tgattttttg aggtaacaag atgcaagttt cagttgaaac cactcaaggc
     1921 cttggccgcc gtgtaacgat tactatcgct gctgacagca tcgagaccgc tgttaaaagc
     1981 gagctggtca acgttgcgaa aaaagtacgt attgacggct tccgcaaagg caaagtgcca
     2041 atgaatatcg ttgctcagcg ttatggcgcg tctgtacgcc aggacgttct gggtgacctg
     2101 atgagccgta acttcattga cgccatcatt aaagaaaaaa tcaatccggc tggcgcaccg
     2161 acttatgttc cgggcgaata caagctgggt gaagacttca cttactctgt agagtttgaa
     2221 gtttatccgg aagttgaact gcagggtctg gaagcgatcg aagttgaaaa accgatcgtt
     2281 gaagtgaccg acgctgacgt tgacggcatg ctggatactc tgcgtaaaca gcaggcgacc
     2341 tggaaagaaa aagacggcgc tgttgaagca gaagaccgcg taaccatcga cttcaccggt
     2401 tctgtagacg gcgaagagtt cgaaggcggt aaagcgtctg atttcgtact ggcgatgggc
     2461 cagggtcgta tgatcccggg ctttgaagac ggtatcaaag gccacaaagc tggcgaagag
     2521 ttcaccatcg acgtgacctt cccggaagaa taccacgcag aaaacctgaa aggtaaagca
     2581 gcgaaattcg ctatcaacct gaagaaagtt gaagagcgtg aactgccgga actgactgca
     2641 gaattcatca aacgtttcgg cgttgaagat ggttccgtag aaggtctgcg cgctgaagtg
     2701 cgtaaaaaca tggagcgcga gctgaagagc gccatccgta accgcgttaa gtctcaggcg
     2761 atcgaaggtc tggtaaaagc taacgacatc gacgtaccgg ctgcgctgat cgacagcgaa
     2821 atcgacgttc tgcgtcgcca ggctgcacag cgtttcggtg gcaacgaaaa acaagctctg
     2881 gaactgccgc gcgaactgtt cgaagaacag gctaaacgcc gcgtagttgt tggcctgctg
     2941 ctgggcgaag ttatccgcac caacgagctg aaagctgacg aagagcgcgt gaaaggcctg
     3001 atcgaagaga tggcttctgc gtacgaagat ccgaaagaag ttatcgagtt ctacagcaaa
     3061 aacaaagaac tgatggacaa catgcgcaat gttgctctgg aagaacaggc tgttgaagct
     3121 gtactggcga aagcgaaagt gactgaaaaa gaaaccactt tcaacgagct gatgaaccag
     3181 caggcgtaat ttacgcagca taacgcgcta aattcgcaca aaggcccgtc accgccaggt
     3241 ggtgggcttt tttttgtcat gaattttgca tggaaccgtg cgaaaagcct ctttcggtgt
     3301 tagcgtaaca acaaaagatt gttatgcttg aaatatggtg atgccgtacc cataacacag
     3361 ggactagctg ataatccgtc cataaggtta caatcggtac agcaggtttt ttcaatttta
     3421 tccaggagac ggaaatgtca tacagcggcg aacgagataa ctttgcaccc catagatctg
     3481 gtaccaagct tatcgatgat aagctgtcaa acatgagaat tacaacttat atcgtatggg
     3541 gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga aatattttat
     3601 ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga
     3661 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt
     3721 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc
     3781 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca
     3841 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     3901 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc
     3961 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
     4021 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg aaacgcctgg
     4081 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc
     4141 ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt
     4201 taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc atttccgctc
     4261 gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt
     4321 atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat gaagcacttc
     4381 actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta gcgctgatgt
     4441 ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg
     4501 atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt
     4561 ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc
     4621 agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg
     4681 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
     4741 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
     4801 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     4861 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     4921 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     4981 tcttgcccgc ctgatgaatg ctcatccgga attc
//
