LOCUS       V013852                 6979 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013852
VERSION     V013852
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    405..423
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             490..1206
                     /gene="lasR"
                     /label="Transcriptional activator protein LasR"
                     /note="Transcriptional activator protein LasR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P25084"
     terminator      1221..1292
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1308..1335
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             1551..4595
                     /label="lacZ"
                     /note="beta-galactosidase"
     terminator      complement(4616..4659)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(4758..4774)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4782..4798)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4806..4836)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4851..4872)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5160..5748)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5922..6779)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6780..6884)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcccta tcagtgatag
      421 agattgacat ccctatcagt gatagagata ctgagcacgt acaaaagagg agaaacctct
      481 agaaataata tggctctggt tgacggtttc ctggaactgg aacgttcttc tggtaaactg
      541 gaatggtctg ctatcctgca gaaaatggct tctgacctgg gtttctctaa aatcctgttc
      601 ggtctgctgc cgaaagactc tcaggactac gaaaacgctt tcatcgttgg taactacccg
      661 gctgcttggc gtgaacacta cgaccgtgct ggttacgctc gtgttgaccc gaccgtttct
      721 cactgcaccc agtctgttct gccgatcttc tgggaaccgt ctatctacca gacccgtaaa
      781 cagcacgaat ttttcgaaga agcttctgct gctggtctgg tttacggtct gaccatgccg
      841 ctgcacggtg ctcgtggtga actgggtgct ctgtctctgt ctgttgaagc tgaaaaccgt
      901 gctgaagcta accgtttcat cgaatctgtt ctgccgaccc tgtggatgct gaaagactac
      961 gctctgcagt ctggtgctgg tctggctttc gaacacccgg tttctaaacc ggttgttctg
     1021 acctctcgtg aaaaagaagt tctgcagtgg tgcgctatcg gtaaaacctc ttgggaaatc
     1081 tctgttatct gcaactgctc tgaagctaac gttaacttcc acatgggtaa catccgtcgt
     1141 aaattcggtg ttacctctcg tcgtgttgct gctatcatgg ctgttaacct gggtctgatc
     1201 accctgtaat aaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1261 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1321 gtgggccttt ctgcgtttat aagtcagtag catgcatgac cacctgtagg atcgtacagg
     1381 tttacgcaag aaaatggttt gttatagtcg aataaaagtc gatatggaaa gaggagaaag
     1441 ccatatctga attcatgtcg tttactttga ccaacaagaa cgtgattttc gttgccggtc
     1501 tgggaggcat tggtctggac accagcaagg agctgctcaa gcgcgatccc gtcgttttac
     1561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1681 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
     1741 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
     1801 tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
     1861 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
     1921 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
     1981 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
     2041 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga
     2101 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
     2161 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
     2221 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
     2281 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
     2341 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
     2401 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
     2461 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
     2521 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
     2581 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
     2641 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
     2701 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
     2761 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
     2821 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
     2881 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
     2941 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
     3001 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
     3061 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
     3121 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
     3181 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
     3241 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
     3301 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
     3361 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
     3421 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
     3481 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
     3541 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
     3601 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
     3661 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
     3721 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
     3781 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
     3841 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
     3901 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
     3961 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
     4021 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
     4081 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
     4141 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
     4201 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
     4261 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
     4321 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
     4381 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
     4441 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
     4501 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattacag ctgagcgccg
     4561 gtcgctacca ttaccagttg gtctggtgtc aaaaataagg taccccaggc atcaaataaa
     4621 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     4681 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggatccc
     4741 cggagctcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4801 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4861 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4921 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5341 gtggcgcttt ctcaaagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6901 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     6961 atcacgaggc cctttcgtc
//
