LOCUS       V013513                 4555 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013513
VERSION     V013513
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    405..423
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             490..1206
                     /gene="lasR"
                     /label="Transcriptional activator protein LasR"
                     /note="Transcriptional activator protein LasR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P25084"
     terminator      1221..1292
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1308..1335
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             1455..2171
                     /label="EGFP"
                     /note="enhanced GFP"
     terminator      complement(2192..2235)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(2334..2350)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2358..2374)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2382..2412)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2427..2448)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2736..3324)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3498..4355)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4356..4460)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcccta tcagtgatag
      421 agattgacat ccctatcagt gatagagata ctgagcacgt acaaaagagg agaaacctct
      481 agaaataata tggctctggt tgacggtttc ctggaactgg aacgttcttc tggtaaactg
      541 gaatggtctg ctatcctgca gaaaatggct tctgacctgg gtttctctaa aatcctgttc
      601 ggtctgctgc cgaaagactc tcaggactac gaaaacgctt tcatcgttgg taactacccg
      661 gctgcttggc gtgaacacta cgaccgtgct ggttacgctc gtgttgaccc gaccgtttct
      721 cactgcaccc agtctgttct gccgatcttc tgggaaccgt ctatctacca gacccgtaaa
      781 cagcacgaat ttttcgaaga agcttctgct gctggtctgg tttacggtct gaccatgccg
      841 ctgcacggtg ctcgtggtga actgggtgct ctgtctctgt ctgttgaagc tgaaaaccgt
      901 gctgaagcta accgtttcat cgaatctgtt ctgccgaccc tgtggatgct gaaagactac
      961 gctctgcagt ctggtgctgg tctggctttc gaacacccgg tttctaaacc ggttgttctg
     1021 acctctcgtg aaaaagaagt tctgcagtgg tgcgctatcg gtaaaacctc ttgggaaatc
     1081 tctgttatct gcaactgctc tgaagctaac gttaacttcc acatgggtaa catccgtcgt
     1141 aaattcggtg ttacctctcg tcgtgttgct gctatcatgg ctgttaacct gggtctgatc
     1201 accctgtaat aaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1261 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1321 gtgggccttt ctgcgtttat aagtcagtag catgcatgac cacctgtagg atcgtacagg
     1381 tttacgcaag aaaatggttt gttatagtcg aataaaagtc gatatggaaa gaggagaaag
     1441 ccatatctga attcatggtt tctaaaggtg aagaactgtt caccggtgtt gttccgatcc
     1501 tggttgaact ggacggtgac gttaacggtc acaaattctc tgtttctggt gaaggtgaag
     1561 gtgacgctac ctacggtaaa ctgaccctga aattcatctg caccaccggt aaactgccgg
     1621 ttccgtggcc gaccctggtt accaccctga cctacggtgt tcagtgcttc tctcgttacc
     1681 cggaccacat gaaacagcac gacttcttca aatctgctat gccggaaggt tacgttcagg
     1741 aacgtaccat cttcttcaaa gacgacggta actacaaaac ccgtgctgaa gttaaattcg
     1801 aaggtgacac cctggttaac cgtatcgaac tgaaaggtat cgacttcaaa gaagacggta
     1861 acatcctggg tcacaaactg gaatacaact acaactctca caacgtttac atcatggctg
     1921 acaaacagaa aaacggtatc aaagttaact tcaaaatccg tcacaacatc gaagacggtt
     1981 ctgttcagct ggctgaccac taccagcaga acaccccgat cggtgacggt ccggttctgc
     2041 tgccggacaa ccactacctg tctacccagt ctgctctgtc taaagacccg aacgaaaaac
     2101 gtgaccacat ggttctgctg gaatttgtta ccgctgctgg tatcaccctg ggtatggacg
     2161 aactgtacaa ataaggtacc ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2221 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2281 accttcgggt gggcctttct gcgtttatag gatccccgga gctcgtaatc atggtcatag
     2341 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2401 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2461 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2521 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca aagctcacgc
     2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4501 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
