LOCUS       40924_42979        4906 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for co-expressing ZsGreen1 with the Tet-Off(R) Advanced 
            transcriptional activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        314..517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             634..1377
                     /codon_start=1
                     /label=tTA-Advanced
                     /note="improved tetracycline-controlled transactivator"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDLDLDMLPG"
     misc_feature    1392..1978
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1979..2671
                     /codon_start=1
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    2820..2901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3083..3671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3845..4702)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4703..4807)
                     /label=AmpR promoter
ORIGIN
        1 ctcgaggtcc gttacataac ttacgctaaa tggcccgcct ggctgaccgc ccaacgaccc
       61 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      121 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      181 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      241 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      301 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      361 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      421 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      481 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
      541 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      601 ccgcggggat cctctagtca gctgacgcgt cctatgtcta gactggacaa gagcaaagtc
      661 ataaactctg ctctggaatt actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa
      721 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
      781 gccctgctcg atgccctggc aatcgagatg ctggacaggc atcataccca cttctgcccc
      841 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
      901 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
      961 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     1021 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggatcag
     1081 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     1141 ctgagacaag caattgagct gttcgaccat cagggagccg aacctgcctt ccttttcggc
     1201 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc
     1261 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     1321 cttgatatgc tgcctgctga cgctcttgac gatttggacc ttgacatgct ccccgggtaa
     1381 ctagaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     1441 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     1501 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     1561 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     1621 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     1681 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     1741 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     1801 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     1861 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     1921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     1981 ggcccagtcc aagcacggcc tgaccaagga gatgaccatg aagtaccgca tggagggctg
     2041 cgtggacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct tcaagggcaa
     2101 gcaggccatc aacctgtgcg tggtggaggg cggccccttg cccttcgccg aggacatctt
     2161 gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag tacccccagg acatcgtcga
     2221 ctacttcaag aactcctgcc ccgccggcta cacctgggac cgctccttcc tgttcgagga
     2281 cggcgccgtg tgcatctgca acgccgacat caccgtgagc gtggaggaga actgcatgta
     2341 ccacgagtcc aagttctacg gcgtgaactt ccccgccgac ggccccgtga tgaagaagat
     2401 gaccgacaac tgggagccct cctgcgagaa gatcatcccc gtgcccaagc agggcatctt
     2461 gaagggcgac gtgagcatgt acctgctgct gaaggacggt ggccgcttgc gctgccagtt
     2521 cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg cccgactggc acttcatcca
     2581 gcacaagctg acccgcgagg accgcagcga cgccaagaac cagaagtggc acctgaccga
     2641 gcacgccatc gcctccggct ccgccttgcc ctgagcggcc gcatcgataa gcttgtcgac
     2701 gatatctcta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2761 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2821 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     2881 ataaagcatt tttttcactg ccccgagctt cctcgctcac tgactcgctg cgctcggtcg
     2941 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3841 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3901 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3961 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4021 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4081 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4141 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4201 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4261 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4321 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4381 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4441 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4501 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4561 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4621 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4681 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4741 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4801 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4861 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttca
//
