LOCUS       62056_21155        3311 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pTet-33tev, complete sequence.
ACCESSION   MK238517
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-NOV-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     regulatory      713..723
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     CDS             733..1434
                     /label=TEV protease
                     /note="tobacco etch virus protease (Kapust et al., 2001)"
     terminator      1476..1547
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1563..1590
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1752..2297)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2411..2505)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2529..3185)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3186..3288)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aatttcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tctcacacag
      721 gacggatcca tgggtgaaag cctgtttaaa ggtccgcgcg attacaaccc gattagcagc
      781 agcatctgcc atctgaccaa cgaatctgac ggtcatacca ccagtctgta cggtattggt
      841 tttggcccgt tcatcatcac caacaaacac ctgttccgcc gtaacaacgg taccctggtt
      901 gttcaaagcc tgcacggcgt tttcaaagtc aaagacacca ccaccctgca acaacatctg
      961 gttgacggtc gcgatatgat catcatccgc atgccgaaag atttcccgcc gtttccgcag
     1021 aaactgaaat tccgcgaacc gcaacgcgaa gaacgtattt gtctggtcac caccaacttc
     1081 cagaccaaat ccatgagcag catggttagc gataccagtt gtacctttcc gtctggcgac
     1141 ggtattttct ggaaacactg gattcagacc aaagacggtc aatgtggtag tccgctggtt
     1201 tctacccgtg acggctttat tgttggcatt catagcgcga gcaacttcac caacaccaac
     1261 aactacttca ccagcgtccc gaaaaacttc atggaactgc tgaccaacca agaagcccaa
     1321 cagtgggtat ctggttggcg tctgaacgca gattctgttc tgtggggcgg tcacaaagtc
     1381 tttatggtca aaccggaaga accgtttcag ccggtaaaag aagcaaccca gctgtaaaag
     1441 cttgcggccg cactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
     1501 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1561 ctggctcacc ttcgggtggg cctttctgcg tttataccta gggatatatt ccgcttcctc
     1621 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     1681 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     1741 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     1801 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     1861 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     1921 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     1981 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     2041 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     2101 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     2161 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     2221 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     2281 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     2341 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgttac
     2401 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     2461 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     2521 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     2581 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     2641 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2701 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     2761 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2821 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     2881 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2941 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     3001 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3061 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     3121 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3181 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     3241 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     3301 cgccagatat c
//
