LOCUS       62056_21150        3980 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pTet-33tev-33tvmv, complete sequence.
ACCESSION   MK258731
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (05-DEC-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     misc_feature    720..730
                     /label=B0033RBS
                     /note="B0033RBS"
     CDS             746..1447
                     /label=TEV protease
                     /note="tobacco etch virus protease (Kapust et al., 2001)"
     misc_feature    1457..1467
                     /label=B0033RBS
                     /note="B0033RBS"
     misc_feature    1474..2130
                     /label=TVMVp
                     /note="TVMVp"
     terminator      2145..2216
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2232..2259
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2421..2966)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3080..3174)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3198..3854)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3855..3957)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aatttcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgataa agttatttta ccactcccta tcagtgatag agaaaagaat tcgagctcct
      721 cacacaggac ggatccatgg gtagcggtga aagcctgttt aaaggtccgc gcgattacaa
      781 cccgattagc agcagcatct gccatctgac caacgaatct gacggtcata ccaccagtct
      841 gtacggtatt ggttttggcc cgttcatcat caccaacaaa cacctgttcc gccgtaacaa
      901 cggtaccctg gttgttcaaa gcctgcacgg cgttttcaaa gtcaaagaca ccaccaccct
      961 gcaacaacat ctggttgacg gtcgcgatat gatcatcatc cgcatgccga aagatttccc
     1021 gccgtttccg cagaaactga aattccgcga accgcaacgc gaagaacgta tttgtctggt
     1081 caccaccaac ttccagacca aatccatgag cagcatggtt agcgatacca gttgtacctt
     1141 tccgtctggc gacggtattt tctggaaaca ctggattcag accaaagacg gtcaatgtgg
     1201 tagtccgctg gtttctaccc gtgacggctt tattgttggc attcatagcg cgagcaactt
     1261 caccaacacc aacaactact tcaccagcgt cccgaaaaac ttcatggaac tgctgaccaa
     1321 ccaagaagcc caacagtggg tatctggttg gcgtctgaac gcagattctg ttctgtgggg
     1381 cggtcacaaa gtctttatgg tcaaaccgga agaaccgttt cagccggtaa aagaagcaac
     1441 ccagctgtaa ggtacctcac acaggacggt acgatgagta aagccctgct gaaaggtgtg
     1501 cgcgatttca acccgattag cgcctgcgtt tgcctgctgg aaaatagctc cgacggtcat
     1561 agcgaacgtc tgtttggcat cggctttggt ccgtacatca tcgcgaacca gcacctgttt
     1621 cgtcgcaaca acggcgaact gaccatcaaa accatgcacg gcgagttcaa agtcaaaaac
     1681 agcacgcagc tgcagatgaa accggttgaa ggtcgcgaca tcatcgtgat caaaatggcg
     1741 aaagacttcc cgccgtttcc gcagaaactg aaattccgtc agccgaccat taaagatcgc
     1801 gtttgcatgg tcagcaccaa cttccagcag aaaagcgtga gtagcctggt tagcgaaagt
     1861 agccacatcg tccacaaaga ggataccagc ttctggcagc attggattac gaccaaagac
     1921 ggtcagtgcg gtagtccgct ggtaagcatc attgacggca acattctggg tattcatagc
     1981 ctgacccata ccaccaacgg cagcaactac ttcgtcgaat tcccggaaaa attcgttgcg
     2041 acctatctgg acgctgccga cggttggtgc aaaaactgga aattcaacgc cgacaaaatc
     2101 tcctggggca gttttaccct ggttgaataa ggatctccag gcatcaaata aaacgaaagg
     2161 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2221 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacctag ggatatattc
     2281 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     2341 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     2401 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     2461 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2521 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     2581 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     2641 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     2701 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     2761 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     2821 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     2881 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     2941 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     3001 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
     3061 agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     3121 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     3181 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     3241 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     3301 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     3361 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     3421 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3481 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     3541 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     3601 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     3661 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     3721 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     3781 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     3841 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     3901 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     3961 tctcattttc gccagatatc
//
