LOCUS       62056_21140        4903 bp DNA     circular SYN 25-MAR-2019
DEFINITION  Cloning vector pTet-(33sug)TEV-(33teg)TVMV-(33tvg)SuMMV, complete 
            sequence.
ACCESSION   MK238520
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2018) State Key Laboratory of Food Science and 
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 
            214122, China
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-NOV-2018) State Key Laboratory of Food Science and Technology, 
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     regulatory      720..730
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    746..763
                     /label=summv protease site
                     /note="summv protease site"
     misc_feature    764..799
                     /label=beta gal degradation signal
                     /note="beta gal degradation signal"
     CDS             800..1501
                     /label=TEV protease
                     /note="tobacco etch virus protease (Kapust et al., 2001)"
     regulatory      1511..1521
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1531..1548
                     /label=tev protease site
                     /note="tev protease site"
     misc_feature    1549..1584
                     /label=beta gal degradation signal
                     /note="beta gal degradation signal"
     misc_feature    1585..2238
                     /label=TVMVp
                     /note="TVMVp"
     regulatory      2245..2255
                     /label=B0033
                     /note="B0033"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    2265..2282
                     /label=TVMV protease site
                     /note="TVMV protease site"
     misc_feature    2283..2318
                     /label=beta gal degradation signal
                     /note="beta gal degradation signal"
     misc_feature    2319..3044
                     /label=SuMMVp
                     /note="SuMMVp"
     terminator      3068..3139
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3155..3182
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3344..3889)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(4003..4097)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4121..4777)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4778..4880)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aatttcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgataa agttatttta ccactcccta tcagtgatag agaaaagaat tcgagctcct
      721 cacacaggac ggatccatgg gtagcgaaga aattcatctg cagttccatg gcagcggtgc
      781 ttggctgctg cctgtgagcg gtgaaagcct gtttaaaggt ccgcgcgatt acaacccgat
      841 tagcagcagc atctgccatc tgaccaacga atctgacggt cataccacca gtctgtacgg
      901 tattggtttt ggcccgttca tcatcaccaa caaacacctg ttccgccgta acaacggtac
      961 cctggttgtt caaagcctgc acggcgtttt caaagtcaaa gacaccacca ccctgcaaca
     1021 acatctggtt gacggtcgcg atatgatcat catccgcatg ccgaaagatt tcccgccgtt
     1081 tccgcagaaa ctgaaattcc gcgaaccgca acgcgaagaa cgtatttgtc tggtcaccac
     1141 caacttccag accaaatcca tgagcagcat ggttagcgat accagttgta cctttccgtc
     1201 tggcgacggt attttctgga aacactggat tcagaccaaa gacggtcaat gtggtagtcc
     1261 gctggtttct acccgtgacg gctttattgt tggcattcat agcgcgagca acttcaccaa
     1321 caccaacaac tacttcacca gcgtcccgaa aaacttcatg gaactgctga ccaaccaaga
     1381 agcccaacag tgggtatctg gttggcgtct gaacgcagat tctgttctgt ggggcggtca
     1441 caaagtcttt atggtcaaac cggaagaacc gtttcagccg gtaaaagaag caacccagct
     1501 gtaaggtacc tcacacagga cggtacgatg gaaaacctgt attttcagtt ccatggcagc
     1561 ggtgcttggc tgctgcctgt gagcagtaaa gccctgctga aaggtgtgcg cgatttcaac
     1621 ccgattagcg cctgcgtttg cctgctggaa aatagctccg acggtcatag cgaacgtctg
     1681 tttggcatcg gctttggtcc gtacatcatc gcgaaccagc acctgtttcg tcgcaacaac
     1741 ggcgaactga ccatcaaaac catgcacggc gagttcaaag tcaaaaacag cacgcagctg
     1801 cagatgaaac cggttgaagg tcgcgacatc atcgtgatca aaatggcgaa agacttcccg
     1861 ccgtttccgc agaaactgaa attccgtcag ccgaccatta aagatcgcgt ttgcatggtc
     1921 agcaccaact tccagcagaa aagcgtgagt agcctggtta gcgaaagtag ccacatcgtc
     1981 cacaaagagg ataccagctt ctggcagcat tggattacga ccaaagacgg tcagtgcggt
     2041 agtccgctgg taagcatcat tgacggcaac attctgggta ttcatagcct gacccatacc
     2101 accaacggca gcaactactt cgtcgaattc ccggaaaaat tcgttgcgac ctatctggac
     2161 gctgccgacg gttggtgcaa aaactggaaa ttcaacgccg acaaaatctc ctggggcagt
     2221 tttaccctgg ttgaataaag atcttcacac aggacggtag catggagacc gtgcgcttcc
     2281 agttccatgg cagcggtgct tggctgctgc ctgtgagcgg agtcagtctt agtcgtgggg
     2341 tacgtgacta taacgctatc tcgtctatgg tctgtcgtgt cacgaacgac tcggggagca
     2401 gcagtacgac gatgtatgga attggctatg gatgttatat cattacaaat aaacaccttt
     2461 ttcgtgagaa taatgggcgc ctgttaatca catctcacca cggggaatac atttgcaaaa
     2521 acagtgcgtc acttaaactt agcctggttc caggacgtga catgctgctt attcgtttgc
     2581 ccaaggattg cccacccttc ccatctaaat taaaattccg tgagccaact tcggaagaaa
     2641 aagcggtact tgtggttacc aatttccaag aaaaacactt gtcatctatg gtatccgagt
     2701 cgtcgtgcgt ggtgcagcgt gaagattcgc ctatctggcg ccactggatc tcgaccaaag
     2761 acgggcattg cggagcgcca atcgtgtcca ttcgtgatgg ttatatcatc ggatctcact
     2821 gtggcgagaa cccgatgacc tcaaacttct ttaccagtat cccgaaggat tttcagaact
     2881 tgcttaatgg aaaagaggcc aatgaatggg tgtccggatg gaaatacaac atcgatgcgg
     2941 tatgctgggg agggctgtca gtcgttaatg acgcaccgag tgaaccattt atcacggcaa
     3001 aagttgttag cgcactggat acggaaggta ttaaagtgca gtaaaagctt taaggatctc
     3061 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3121 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3181 cgtttatacc tagggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     3241 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     3301 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     3361 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     3421 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     3481 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     3541 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     3601 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     3661 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     3721 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     3781 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     3841 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     3901 tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc
     3961 tgaagtcagc cccatacgat ataagttgtt actagtgctt ggattctcac caataaaaaa
     4021 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     4081 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     4141 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     4201 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     4261 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     4321 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     4381 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     4441 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     4501 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     4561 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     4621 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     4681 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     4741 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     4801 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     4861 aacctcttac gtgccgatca acgtctcatt ttcgccagat atc
//
