LOCUS       62056_21135        2923 bp DNA     circular SYN 28-JUL-2021
DEFINITION  Cloning vector pTestOR, complete sequence.
ACCESSION   MZ395127
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Becker K, Meyer A, Roberts TM, Panke S.
  TITLE     Plasmid replication based on the T7 origin of replication requires a
            T7 RNAP variant and inactivation of ribonuclease H
  JOURNAL   Nucleic Acids Res (2021) In press
  PUBMED    34255845
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Becker K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2021) D-BSSE, ETHZ, Mattenstrasse 26, Basel, BS 
            4058, Switzerland
REFERENCE   3  (bases 1 to 2923)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUN-2021) D-BSSE, ETHZ, Mattenstrasse 26, Basel, BS 4058, 
            Switzerland"
FEATURES             Location/Qualifiers
     source          1..2923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      240..462
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     promoter        complement(1422..1440)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1596..1612)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2059..2715)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2716..2818)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat
       61 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg
      121 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta
      181 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg
      241 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat
      301 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag
      361 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca
      421 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg
      481 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg
      541 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg
      601 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca
      661 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag
      721 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag
      781 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag
      841 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc
      901 tggaacatgt atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac
      961 gagtttttgg tgcatttaaa gctgttcacc atgaacagat cgacaatgta acagatgaac
     1021 agcatgtaac acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa
     1081 ttgaacacct gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg
     1141 cgatgctgct tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg
     1201 ggaaaaaatc atggcaattc tggaagaaat agcgcccaat acgcaaaccg cctctccccg
     1261 cgcgttggcc gattcattat gttggtcctt aagttaaggt gatactaata caatataatc
     1321 accattctta gaggtcactc taggtacacc attatgcata ctttatgcac tctataagta
     1381 ttcattcagt atctatatag taacctttcg tccctcctct ccctatagtg agtcgtatta
     1441 atttatcgtg tcttttcagt aacttaaaga actgaccatc cgtggtctat agtgcatccc
     1501 tatgcagtcc taatgcataa atcaccactc aatgaaagac gtaccgtaac cctcatcgtc
     1561 tgcaggcatg caagcttgcg gccgcccagc ttggcactgg ccgtcgtttt acaacgtcgt
     1621 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     1681 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     1741 aatggcgaat ggcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat
     1801 cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc
     1861 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
     1921 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
     1981 attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata
     2041 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     2101 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     2161 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     2221 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     2281 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     2341 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     2401 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     2461 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     2521 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     2581 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     2641 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     2701 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     2761 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     2821 gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt
     2881 tatttattct gcgaagtgat cttccgtcac aggtatttat tcg
//
