LOCUS       40924_42874        3116 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-ble-plus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3116)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3116)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             267..638
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVENDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(1395..1983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2157..3014)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3015..3116,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaacgagtt
       61 tcttcaaaat atgcagcgga ttcactaaga aacattttca cgcacgaaag cgaaattatt
      121 atgattgtta ttataatact ttttctttgt tgttttatcc acttattatt gttgtgttaa
      181 attgttttta ccttttttct tttccaactt cttttatgat gtcttttctt tttttttttt
      241 gctctataag ttgtcttgtc tagaaaatgg ccaagttgac cagtgccgtt ccggtgctca
      301 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
      361 acttcgtgga gaacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
      421 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
      481 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
      541 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
      601 ccggcaactg cgtgcacttc gtggccgagg agcaggacta ggcgtggcga tgacttcagg
      661 tctttctttt gcgaataggg atcttataat acacgatgcg tgtcccgtga tggtcgttac
      721 cggtgctgcc acgatccaag tgacacagtg tcaagagcaa aacaatttta cttttccctt
      781 taaggacaac aacaaaaaaa tatataactt ttttttcttt tttttttttg aaattatatt
      841 tatggtcatc tttgggaaac aaaaagcagc aatttaatga tgcggaagga tgagtgaaat
      901 aatgtttaat caatgtacga ggatttgggg tattgcaagg aaaatgtaga tgatttgatt
      961 gggtgtatga tgcagcttgt ggtaattttt tacaactttg cgttattgga agattttggt
     1021 tgttcagttt ctttttgttt ataactgact gttttaattt agtgtgattt ttttttttaa
     1081 ggatttaagc tgtttttctt tttttttctt tttcttcact gatggtgttt aaggtgtagt
     1141 ttagtgtttg tgcattaagc ttcatgatcg atgcatgtac aattgctagc tcgaggatcc
     1201 agctgcggcc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1801 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2041 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2101 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttcc
//
