LOCUS       40924_42914        3340 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-PAC-plus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3340)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             266..862
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVEVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPEHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     rep_origin      complement(1619..2207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2381..3238)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3239..3340,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaacgagtt
       61 tcttcaaaat atgcagcgga ttcactaaga aacattttca cgcacgaaag cgaaattatt
      121 atgattgtta ttataatact ttttctttgt tgttttatcc acttattatt gttgtgttaa
      181 attgttttta ccttttttct tttccaactt cttttatgat gtcttttctt tttttttttg
      241 ctctataagt tgtcttgtct agaaaatgac cgagtacaag cccacggtgc gcctcgccac
      301 ccgcgacgac gtcccccggg ccgtacgcac cctcgcagcc gcgttcgccg actaccccgc
      361 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
      421 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
      481 ggtggaggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
      541 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
      601 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
      661 cgaacaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
      721 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
      781 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
      841 catgacccgc aagcccggtg cctaggcgtg gcgatgactt caggtctttc ttttgcgaat
      901 agggatctta taatacacga tgcgtgtccc gtgatggtcg ttaccggtgc tgccacgatc
      961 caagtgacac agtgtcaaga gcaaaacaat tttacttttc cctttaagga caacaacaaa
     1021 aaaatatata actttttttt cttttttttt tttgaaatta tatttatggt catctttggg
     1081 aaacaaaaag cagcaattta atgatgcgga aggatgagtg aaataatgtt taatcaatgt
     1141 acgaggattt ggggtattgc aaggaaaatg tagatgattt gattgggtgt atgatgcagc
     1201 ttgtggtaat tttttacaac tttgcgttat tggaagattt tggttgttca gtttcttttt
     1261 gtttataact gactgtttta atttagtgtg attttttttt ttaaggattt aagctgtttt
     1321 tctttttttt tctttttctt cactgatggt gtttaaggtg tagtttagtg tttgtgcatt
     1381 aagcttcatg atcgatgcat gtacaattgc tagctcgagg atccagctgc ggccgctctt
     1441 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1501 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2041 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3301 tatttgaatg tatttagaaa aataaacaaa taggggttcc
//
