LOCUS       40924_42909        3340 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-PAC-minus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3340)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(571..1167)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVEVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPEHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     rep_origin      complement(1619..2207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2381..3238)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3239..3340,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaatgcaca
       61 aacactaaac tacaccttaa acaccatcag tgaagaaaaa gaaaaaaaaa gaaaaacagc
      121 ttaaatcctt aaaaaaaaaa atcacactaa attaaaacag tcagttataa acaaaaagaa
      181 actgaacaac caaaatcttc caataacgca aagttgtaaa aaattaccac aagctgcatc
      241 atacacccaa tcaaatcatc tacattttcc ttgcaatacc ccaaatcctc gtacattgat
      301 taaacattat ttcactcatc cttccgcatc attaaattgc tgctttttgt ttcccaaaga
      361 tgaccataaa tataatttca aaaaaaaaaa agaaaaaaaa gttatatatt tttttgttgt
      421 tgtccttaaa gggaaaagta aaattgtttt gctcttgaca ctgtgtcact tggatcgtgg
      481 cagcaccggt aacgaccatc acgggacacg catcgtgtat tataagatcc ctattcgcaa
      541 aagaaagacc tgaagtcatc gccacgccta ggcaccgggc ttgcgggtca tgcaccaggt
      601 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
      661 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
      721 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtgtt cgggcgagac
      781 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
      841 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
      901 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagacctcca ccgcggcgcc
      961 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     1021 cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta
     1081 gtcggcgaac gcggctgcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag
     1141 gcgcaccgtg ggcttgtact cggtcatttt ctagacaaga caacttatag agcaaaaaaa
     1201 aaaagaaaag acatcataaa agaagttgga aaagaaaaaa ggtaaaaaca atttaacaca
     1261 acaataataa gtggataaaa caacaaagaa aaagtattat aataacaatc ataataattt
     1321 cgctttcgtg cgtgaaaatg tttcttagtg aatccgctgc atattttgaa gaaactcgtt
     1381 aagcttcatg atcgatgcat gtacaattgc tagctcgagg atccagctgc ggccgctctt
     1441 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1501 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2041 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3301 tatttgaatg tatttagaaa aataaacaaa taggggttcc
//
