LOCUS       40924_42894        3767 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-Hyg-plus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3767)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3767)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             267..1289
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     D"
     rep_origin      complement(2046..2634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2808..3665)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3666..3767,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaacgagtt
       61 tcttcaaaat atgcagcgga ttcactaaga aacattttca cgcacgaaag cgaaattatt
      121 atgattgtta ttataatact ttttctttgt tgttttatcc acttattatt gttgtgttaa
      181 attgttttta ccttttttct tttccaactt cttttatgat gtcttttctt tttttttttt
      241 gctctataag ttgtcttgtc tagaaaatga aaaagcctga actcaccgcg acgtctgtcg
      301 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
      361 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
      421 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
      481 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
      541 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
      601 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
      661 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
      721 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
      781 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
      841 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
      901 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
      961 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     1021 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     1081 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     1141 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     1201 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     1261 gccccagcac tcgtccgagg gcaaaggact aggcgtggcg atgacttcag gtctttcttt
     1321 tgcgaatagg gatcttataa tacacgatgc gtgtcccgtg atggtcgtta ccggtgctgc
     1381 cacgatccaa gtgacacagt gtcaagagca aaacaatttt acttttccct ttaaggacaa
     1441 caacaaaaaa atatataact tttttttctt tttttttttt gaaattatat ttatggtcat
     1501 ctttgggaaa caaaaagcag caatttaatg atgcggaagg atgagtgaaa taatgtttaa
     1561 tcaatgtacg aggatttggg gtattgtaag gaaaatgtag atgatttgat tgggtgtatg
     1621 atgcagcttg tggtaatttt ttacaacttt gcgttattgg aagattttgg ttgttcagtt
     1681 tctttttgtt tataactgac tgttttaatt tagtgtgatt ttttttttta aggatttaag
     1741 ctgtttttct ttttttttct ttttcttcac tgatggtgtt taaggtgtag tttagtgttt
     1801 gtgcattaag cttcatgatc gatgcatgta caattgctag ctcgaggatc cagctgcggc
     1861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2101 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2161 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2221 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2281 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2341 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2401 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2461 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     2521 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2581 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2641 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2701 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2761 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2821 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2881 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2941 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3001 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3061 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3121 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3181 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3241 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3301 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3361 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3421 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3481 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3541 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3601 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3661 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3721 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttcc
//
