LOCUS       40924_42889        3767 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-Hyg-minus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3767)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3767)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(571..1593)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     D"
     rep_origin      complement(2046..2634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2808..3665)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3666..3767,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaatgcaca
       61 aacactaaac tacaccttaa acaccatcag tgaagaaaaa gaaaaaaaaa gaaaaacagc
      121 ttaaatcctt aaaaaaaaaa atcacactaa attaaaacag tcagttataa acaaaaagaa
      181 actgaacaac caaaatcttc caataacgca aagttgtaaa aaattaccac aagctgcatc
      241 atacacccaa tcaaatcatc tacattttcc ttacaatacc ccaaatcctc gtacattgat
      301 taaacattat ttcactcatc cttccgcatc attaaattgc tgctttttgt ttcccaaaga
      361 tgaccataaa tataatttca aaaaaaaaaa agaaaaaaaa gttatatatt tttttgttgt
      421 tgtccttaaa gggaaaagta aaattgtttt gctcttgaca ctgtgtcact tggatcgtgg
      481 cagcaccggt aacgaccatc acgggacacg catcgtgtat tataagatcc ctattcgcaa
      541 aagaaagacc tgaagtcatc gccacgccta gtcctttgcc ctcggacgag tgctggggcg
      601 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
      661 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
      721 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
      781 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
      841 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
      901 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
      961 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
     1021 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
     1081 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
     1141 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
     1201 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
     1261 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
     1321 gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag
     1381 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
     1441 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
     1501 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
     1561 gacagacgtc gcggtgagtt caggcttttt cattttctag acaagacaac ttatagagca
     1621 aaaaaaaaaa agaaaagaca tcataaaaga agttggaaaa gaaaaaaggt aaaaacaatt
     1681 taacacaaca ataataagtg gataaaacaa caaagaaaaa gtattataat aacaatcata
     1741 ataatttcgc tttcgtgcgt gaaaatgttt cttagtgaat ccgctgcata ttttgaagaa
     1801 actcgttaag cttcatgatc gatgcatgta caattgctag ctcgaggatc cagctgcggc
     1861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2101 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2161 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2221 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2281 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2341 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2401 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2461 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     2521 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2581 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2641 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2701 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2761 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2821 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2881 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2941 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3001 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3061 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3121 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3181 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3241 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3301 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3361 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3421 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3481 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3541 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3601 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3661 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3721 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttcc
//
