LOCUS       40924_42884        3141 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-BSD-plus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             267..662
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     rep_origin      complement(1420..2008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2182..3039)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3040..3141,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaacgagtt
       61 tcttcaaaat atgcagcgga ttcactaaga aacattttca cgcacgaaag cgaaattatt
      121 atgattgtta ttataatact ttttctttgt tgttttatcc acttattatt gttgtgttaa
      181 attgttttta ccttttttct tttccaactt cttttatgat gtcttttctt tttttttttt
      241 gctctataag ttgtcttgtc tagaaaatgg ccaagccttt gtctcaagaa gaatccaccc
      301 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
      361 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
      421 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
      481 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
      541 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
      601 atggacagcc gacggcagtt gggattcgtg aactgctgcc ctctggttat gtgtgggagg
      661 gctaggcgtg gcgatgactt caggtctttc ttttgcgaat agggatctta taatacacga
      721 tgcgtgtccc gtgatggtcg ttaccggtgc tgccacgatc caagtgacac agtgtcaaga
      781 gcaaaacaat tttacttttc cctttaagga caacaacaaa aaaatatata actttttttt
      841 cttttttttt ttttgaaatt atatttatgg tcatctttgg gaaacaaaaa gcagcaattt
      901 aatgatgcgg aaggatgagt gaaataatgt ttaatcaatg tacgaggatt tggggtattg
      961 caaggaaaat gtagatgatt tgattgggtg tatgatgcag cttgtggtaa ttttttacaa
     1021 ctttgcgtta ttggaagatt ttggttgttc agtttctttt tgtttataac tgactgtttt
     1081 aatttagtgt gatttttttt tttaaggatt taagctgttt ttcttttttt ttctttttct
     1141 tcactgatgg tgtttaaggt gtagtttagt gtttgtgcat taagcttcat gatcgatgca
     1201 tgtacaattg ctagctcgag gatccagctg cggccgctct tccgcttcct cgctcactga
     1261 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1321 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1381 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1441 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1501 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1561 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1621 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1681 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1741 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1801 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1861 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1921 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2101 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2161 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2221 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2281 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2341 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2401 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2461 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2521 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2581 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2641 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2701 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2761 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2821 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2881 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2941 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3001 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3061 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3121 aaataaacaa ataggggttc c
//
