LOCUS       40924_42879        3141 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-BSD-minus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(572..967)
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     rep_origin      complement(1420..2008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2182..3039)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3040..3141,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaatgcaca
       61 aacactaaac tacaccttaa acaccatcag tgaagaaaaa gaaaaaaaaa gaaaaacagc
      121 ttaaatcctt aaaaaaaaaa atcacactaa attaaaacag tcagttataa acaaaaagaa
      181 actgaacaac caaaatcttc caataacgca aagttgtaaa aaattaccac aagctgcatc
      241 atacacccaa tcaaatcatc tacattttcc ttgcaatacc ccaaatcctc gtacattgat
      301 taaacattat ttcactcatc cttccgcatc attaaattgc tgctttttgt ttcccaaaga
      361 tgaccataaa tataatttca aaaaaaaaaa aagaaaaaaa agttatatat ttttttgttg
      421 ttgtccttaa agggaaaagt aaaattgttt tgctcttgac actgtgtcac ttggatcgtg
      481 gcagcaccgg taacgaccat cacgggacac gcatcgtgta ttataagatc cctattcgca
      541 aaagaaagac ctgaagtcat cgccacgcct agccctccca cacataacca gagggcagca
      601 gttcacgaat cccaactgcc gtcggctgtc catcactgtc cttcactatg gctttgatcc
      661 caggatgcag atcgagaagc acctgtcggc accgtccgca ggggctcaag atgcccctgt
      721 tctcatttcc gatcgcgacg atacaagtca ggttgccagc tgccgcagca gcagcagtgc
      781 ccagcaccac gagttctgca caaggtcccc cagtaaaatg atatacattg acaccagtga
      841 agatgcggcc gtcgctagag agagctgcgc tggcgacgct gtagtcttca gagatgggga
      901 tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga gacaaaggct
      961 tggccatttt ctagacaaga caacttatag agcaaaaaaa aaaaagaaaa gacatcataa
     1021 aagaagttgg aaaagaaaaa aggtaaaaac aatttaacac aacaataata agtggataaa
     1081 acaacaaaga aaaagtatta taataacaat cataataatt tcgctttcgt gcgtgaaaat
     1141 gtttcttagt gaatccgctg catattttga agaaactcgt taagcttcat gatcgatgca
     1201 tgtacaattg ctagctcgag gatccagctg cggccgctct tccgcttcct cgctcactga
     1261 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1321 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1381 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1441 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1501 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1561 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1621 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1681 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1741 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1801 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1861 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1921 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2101 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2161 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2221 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2281 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2341 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2401 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2461 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2521 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2581 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2641 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2701 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2761 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2821 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2881 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2941 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3001 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3061 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3121 aaataaacaa ataggggttc c
//
