LOCUS       40924_42819        3348 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-GA-ble-minus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3348)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3348)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(502..873)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVENDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(1627..2215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2389..3246)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3247..3348,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttagaaacaa
       61 aaaaaaaaaa aacgcgatat aaacaaaaaa cctaaaaaag tttttctccc tggtttgttt
      121 gttatgtgaa aaaagaagaa gaaaaaacaa gaaatggaca aagatcaaac cgccgtgagg
      181 gtgagcatca cacacaaaaa aaaagtcaac gaccaccaac aagccatcaa agctcaaagc
      241 tgcaaactcc tatcgactag ctcgctcacg caaacatgga ggaaggtttg aaaaaaaaat
      301 ctcttgaatg ttcagattcc gattatgact ggcttgaaat tattattaaa agctccttca
      361 gtaaatcaac acttgaaaaa aagatatata tatatgggaa cgcgatctca gaaaccttac
      421 aaggacagaa aacggtaagc ccacggaaga aaaataattc cggtgatagg aaaactattc
      481 acctttatgt agagattact agtcctgctc ctcggccacg aagtgcacgc agttgccggc
      541 cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg tcatggccgg
      601 cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt acagctcgtc
      661 caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt cctggaccgc
      721 gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgttct ccacgaagtc
      781 ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt
      841 gagcaccgga acggcactgg tcaacttggc cattttaaat tctgttcaat gtaattgttg
      901 ttggtagatg gctttcaaag taaaaatatc actttctgca ttcgtgcagg aaacgtggga
      961 aaagagagac aagtggaata atggggaaag aaaagcagaa aaactaaaaa gatgtggcaa
     1021 aaagggaagt gagcaaaaat taccacaagc tgcatcacac acccaattaa atcatctaca
     1081 ttttccttgc aataccccaa atcctcgtac attgattaaa cattatttca ctcatccttc
     1141 cgcatcatta aattgctgct ttttgtttcc caaagatgac cataaatata atttcaaaaa
     1201 aaaaaaaaaa gaaaaaaaag ttatatattt ttttgttgtt gtccttaaag ggaaaagtaa
     1261 aattgttttg ctcttgacgc tgtgtcagtt ggatcgtggc agcaccggta acgatcatca
     1321 cgggacacgc atcgtgtatt ataagatccc tattcgcaaa agaaagacct gaagtcatcg
     1381 ccacgcctaa gcttcatgat cgatgcatgt acaattgcta gctcgaggat ccagctgcgg
     1441 ccgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1501 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1561 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1621 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1681 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1741 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1801 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1861 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1921 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1981 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2041 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2101 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2161 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2221 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2281 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2341 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2401 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2461 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2521 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2581 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2641 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2701 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2761 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2821 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2881 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2941 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3001 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3061 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3121 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3181 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3241 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3301 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttcc
//
