LOCUS       40924_42859        3574 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-GA-PAC-minus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(503..1099)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVEVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPEHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     rep_origin      complement(1853..2441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2615..3472)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3473..3574,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttagaaacaa
       61 aaaaaaaaaa aacgcgatat aaacaaaaaa cctaaaaaag tttttctccc tggtttgttt
      121 gttatgtgaa aaaagaagaa gaaaaaacaa gaaatggaca aagatcaaac cgccgtgagg
      181 gtgagcatca cacacaaaaa aaaagtcaac gaccaccaac aagccatcaa agctcaaagc
      241 tgcaaactcc tatcgactag ctcgctcacg caaacatgga ggaaggtttg aaaaaaaaaa
      301 tctcttgaat gttcagattc cgattatgac tggcttgaaa ttattattaa aagctccttc
      361 agtaaatcaa cacttgaaaa aaagatatat atatatggga acgcgatctc agaaacctta
      421 caaggacaga aaacggtaag cccacggaag aaaaataatt ccggtgatag gaaaactatt
      481 cacctttatg tagagattac taggcaccgg gcttgcgggt catgcaccag gtgcgcggtc
      541 cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt
      601 tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc
      661 cggggagcac gacggcgctg cccagaccct tgccctggtg ttcgggcgag acgccgacgg
      721 tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt
      781 gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca
      841 ccgcccccgc ttcgacgctc tccggcgtgg tccagacctc caccgcggcg ccgtcgtccg
      901 cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg
      961 tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg cgtggcgggg tagtcggcga
     1021 acgcggctgc gagggtgcgt acggcccggg ggacgtcgtc gcgggtggcg aggcgcaccg
     1081 tgggcttgta ctcggtcatt ttaaattctg ttcaatgtaa ttgttgttgg tagatggctt
     1141 tcaaagtaaa aatatcactt tctgcattcg tgcaggaaac gtgggaaaag agagacaagt
     1201 ggaataatgg ggaaagaaaa gcagaaaaac taaaaagatg tggcaaaaag ggaagtgagc
     1261 aaaaattacc acaagctgca tcacacaccc aattaaatca tctacatttt ccttgcaata
     1321 ccccaaatcc tcgtacattg attaaacatt atttcactca tccttccgca tcattaaatt
     1381 gctgcttttt gtttcccaaa gatgaccata aatataattt caaaaaaaaa aaaaaagaaa
     1441 aaaaagttat atattttttt gttgttgtcc ttaaagggaa aagtaaaatt gttttgctct
     1501 tgacgctgtg tcagttggat cgtggcagca ccggtaacga tcatcacggg acacgcatcg
     1561 tgtattataa gatccctatt cgcaaaagaa agacctgaag tcatcgccac gcctaagctt
     1621 catgatcgat gcatgtacaa ttgctagctc gaggatccag ctgcggccgc tcttccgctt
     1681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2281 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3541 aatgtattta gaaaaataaa caaatagggg ttcc
//
