LOCUS       40924_42829        3373 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-GA-BSD-minus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3373)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(503..898)
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     rep_origin      complement(1652..2240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2414..3271)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3272..3373,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttagaaacaa
       61 aaaaaaaaaa aacgcgatat aaacaaaaaa cctaaaaaag tttttctccc tggtttgttt
      121 gttatgtgaa aaaagaagaa gaaaaaacaa gaaatggaca aagatcaaac cgccgtgagg
      181 gtgagcatca cacacaaaaa aaaagtcaac gaccaccaac aagccatcaa agctcaaagc
      241 tgcaaactcc tatcgactag ctcgctcacg caaacatgga ggaaggtttg aaaaaaaaaa
      301 tctcttgaat gttcagattc cgattatgac tggcttgaaa ttattattaa aagctccttc
      361 agtaaatcaa cacttgaaaa aaagatatat atatatggga acgcgatctc agaaacctta
      421 caaggacaga aaacggtaag cccacggaag aaaaataatt ccggtgatag gaaaactatt
      481 cacctttatg tagagattac tagccctccc acacataacc agagggcagc agttcacgaa
      541 tcccaactgc cgtcggctgt ccatcactgt ccttcactat ggctttgatc ccaggatgca
      601 gatcgagaag cacctgtcgg caccgtccgc aggggctcaa gatgcccctg ttctcatttc
      661 cgatcgcgac gatacaagtc aggttgccag ctgccgcagc agcagcagtg cccagcacca
      721 cgagttctgc acaaggtccc ccagtaaaat gatatacatt gacaccagtg aagatgcggc
      781 cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc agagatgggg atgctgttga
      841 ttgtagccgt tgctctttca atgagggtgg attcttcttg agacaaaggc ttggccattt
      901 taaattctgt tcaatgtaat tgttgttggt agatggcttt caaagtaaaa atatcacttt
      961 ctgcattcgt gcaggaaacg tgggaaaaga gagacaagtg gaataatggg gaaagaaaag
     1021 cagaaaaact aaaaagatgt ggcaaaaagg gaagtgagca aaaattacca caagctgcat
     1081 cacacaccca attaaatcat ctacattttc cttgcaatac cccaaatcct cgtacattga
     1141 ttaaacatta tttcactcat ccttccgcat cattaaattg ctgctttttg tttcccaaag
     1201 atgaccataa atataatttc aaaaaaaaaa aaaaagaaaa aaaagttata tatttttttg
     1261 ttgttgtcct taaagggaaa agtaaaattg ttttgctctt gacgctgtgt cagttggatc
     1321 gtggcagcac cggtaacgat catcacggga cacgcatcgt gtattataag atccctattc
     1381 gcaaaagaaa gacctgaagt catcgccacg cctaagcttc atgatcgatg catgtacaat
     1441 tgctagctcg aggatccagc tgcggccgct cttccgcttc ctcgctcact gactcgctgc
     1501 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1561 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1621 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1681 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1741 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1801 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1861 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1921 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1981 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2041 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2101 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2161 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2221 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2281 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2341 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2401 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2461 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2521 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2581 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2641 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2701 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2761 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2821 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2881 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2941 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3001 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3061 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3301 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3361 aaataggggt tcc
//
