LOCUS       62056_20990        3197 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             124..912
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1090..1678
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3004..3038
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        3060..3135
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcgagttcat gtgcagctcc ataagcaaaa ggggatgata agtttatcac caccgactat
       61 ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat
      121 gtcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
      181 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
      241 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
      301 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
      361 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
      421 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
      481 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
      541 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
      601 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
      661 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
      721 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
      781 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
      841 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
      901 gtagtcggca aataagatgc cgctcgccag tcgattggct gagctcatga agttcctatt
      961 ccgaagttcc gcgaacgcgt aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1201 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1741 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1801 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1861 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatgggc
     1921 tttgtggcga tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac
     1981 acaaactttg cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg
     2041 tctgaagttg atgaagccct gcaacgcgcc ttctccatcg acggtccggt gttggtggat
     2101 gtggtggtcg ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa
     2161 ggtttcagcc tgtatatgct gcgcgcaatc atcagcggac gcggtgatga agtgatcgaa
     2221 ctggcgaaaa caaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat
     2281 gccatgattg atttacgcag tgataccgtt acccgaccaa gccgcgccat gctcgaagcg
     2341 atgatggccg ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctcct
     2401 gacttagctt cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag
     2461 caggaagtga aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt caccccagta
     2521 gcagaagcgg cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac
     2581 gccccggttg gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct
     2641 gcctgccgtg cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg
     2701 gtgagcagca caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc
     2761 tcctgccatc cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc
     2821 tttcatcggg ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc
     2881 agatgaacta aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg
     2941 gttgcagctt atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagga
     3001 tccttgacag ctagctcagt cctaggtata atactagtgt agtgacagtc ttaatcgcag
     3061 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     3121 gcaccgagtc ggtgcttttt ttgaattctc tagagtcgac ctgcagaagc ttagatctat
     3181 taccctgtta tccctac
//
