LOCUS       40924_42524        3702 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTacCDFS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 3702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..203
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    211..227
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    246..302
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      300..447
                     /label=rrnBT1T2
                     /note="rrnBT1T2"
                     /regulatory_class="terminator"
     CDS             complement(621..1409)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(1410..1501)
                     /label=AmpR promoter
     rep_origin      complement(1549..2287)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(2463..2484)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2500..3579)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3580..3657)
                     /label=lacI promoter
ORIGIN
        1 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
       61 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
      121 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
      181 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      241 acagagaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc
      301 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      361 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
      421 ccatgccgaa ctcagaagtg aaacgccctg aaacctcagg catttgagaa gcacacggtc
      481 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
      541 cgaccctgcc ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc ccctcggctt
      601 gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
      661 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
      721 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
      781 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
      841 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
      901 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
      961 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     1021 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     1081 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     1141 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     1201 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     1261 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     1321 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     1381 gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt
     1441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1501 atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca
     1561 catacaaagt tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca
     1621 gcagggccgc gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa
     1681 acagacgctt ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta
     1741 cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg
     1801 cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg
     1861 gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg
     1921 ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg
     1981 taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat
     2041 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     2101 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
     2161 ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt
     2221 ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
     2281 tttgatcttt tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc
     2341 acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc
     2401 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     2461 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2521 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2581 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     2641 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     2701 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     2761 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     2821 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     2881 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     2941 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     3001 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     3061 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     3121 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     3181 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     3241 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     3301 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     3361 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     3421 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     3481 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     3541 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     3601 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     3661 gatctcgacg ctctccctta tgcgactcct gcattaggaa at
//
