LOCUS       40924_42514        3742 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTac15K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 3742)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..59
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      262..348
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      440..467
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        487..578
                     /label=AmpR promoter
     primer_bind     complement(891..907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1150..1962
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2494..3039
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        3676..3704
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3712..3728
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cagaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg
       61 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
      121 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
      181 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
      241 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      301 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
      361 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
      421 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
      481 actctttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      541 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      601 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      661 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      721 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      781 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
      841 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtaattc actggccgtc
      901 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      961 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1021 cagttgcggg ggggggggga aagccacgtt gtgtctcaaa atctctgatg ttacattgca
     1081 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1141 aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc
     1201 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1261 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1321 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1381 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1441 accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt
     1501 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     1561 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     1621 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     1681 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     1741 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1801 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     1861 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     1921 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaagcatt ggtaacctgg
     1981 tatgagtcag caacaccttc ttcacgaggc agacctcagc gccccccccc cccgcaaact
     2041 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2101 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2161 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2221 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2281 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2341 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2401 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2461 ctgagcgtca gaccccttaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat
     2521 ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc
     2581 taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct
     2641 ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag
     2701 tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac
     2761 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     2821 gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc
     2881 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg
     2941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag
     3001 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc
     3061 tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag
     3121 ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     3181 aatatatcct gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac
     3241 atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc
     3301 tagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     3361 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     3421 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     3481 gtagaggatc cggagcttat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc
     3541 atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa
     3601 ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata
     3661 ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
     3721 gataacaatt tcacacagga aa
//
