LOCUS       40924_42509        4653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTac15K-sfp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 4653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..680
                     /gene="sfp"
                     /label=sfp
                     /note="4'-phosphopantetheinyl transferase Sfp from Bacillus
                     subtilis (strain 168). Accession#: P39135"
     misc_feature    684..740
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      943..1029
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1121..1148
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1168..1259
                     /label=AmpR promoter
     primer_bind     complement(1572..1588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1831..2643
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3405..3950
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        4587..4615
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4623..4639
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cagaattcat gaagatttac ggaatttata tggaccgccc gctttcacag gaagaaaatg
       61 aacggttcat gtctttcata tcacctgaaa aacgggagaa atgccggaga ttttatcata
      121 aagaagatgc tcaccgcacc ctgctgggag atgtgctcgt tcgctcagtc ataagcaggc
      181 agtatcagtt ggacaaatcc gatatccgct ttagcacgca ggaatacggg aagccgtgca
      241 tccctgatct tcccgacgct catttcaaca tttctcactc cggacgctgg gtcatttgcg
      301 cgtttgattc acagccgatc ggcatagata tcgaaaaaac gaaaccgatc agccttgaga
      361 tcgccaagcg cttcttttca aaaacagagt acagcgacct tttagcaaaa gacaaggacg
      421 agcagacaga ctatttttat catctatggt caatgaaaga aagctttatc aaacaggaag
      481 gcaaaggctt atcgcttccg cttgattcct tttcagtgcg cctgcaccag gacggacaag
      541 tatccattga gcttccggac agccattccc catgctatat caaaacgtat gaggtcgatc
      601 ccggctacaa aatggctgta tgcgccgtac accctgattt ccccgaggat atcacaatgg
      661 tctcgtacga agagctttta taagaattcg agctcggtac ccggggatcc tctagagtcg
      721 acctgcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
      781 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
      841 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
      901 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      961 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1021 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     1081 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     1141 ggcctttttg cgtttctaca aactcttttt gtttattttt ctaaatacat tcaaatatgt
     1201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1501 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1561 ttgagtaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1621 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1681 cgcaccgatc gcccttccca acagttgcgg gggggggggg aaagccacgt tgtgtctcaa
     1741 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1801 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
     1861 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1921 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     1981 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2041 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2101 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
     2161 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2221 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2281 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2341 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg
     2401 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2461 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2521 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2581 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2641 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2701 gacggcggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc
     2761 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca
     2821 cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga
     2881 ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag cgcccccccc
     2941 ccccgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3001 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3061 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3121 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3181 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3241 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3301 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3361 ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga tcgttttggt
     3421 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag
     3481 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca
     3541 aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa
     3601 tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag
     3661 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     3721 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     3781 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     3841 agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     3901 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     3961 ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc
     4021 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
     4081 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt
     4141 tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt
     4201 atacactccg ctagcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     4261 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     4321 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     4381 atgcgtccgg cgtagaggat ccggagctta tcgactgcac ggtgcaccaa tgcttctggc
     4441 gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc
     4501 gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt
     4561 tctggcaaat attctgaaat gagctgttga caattaatca tcggctcgta taatgtgtgg
     4621 aattgtgagc ggataacaat ttcacacagg aaa
//
